1887

Abstract

(group B streptococcus, GBS) is an important cause of sepsis in neonates and their mothers, and the elderly and immunocompromised patients. Ongoing surveillance to monitor GBS serotype distribution is needed to guide the development and assess the feasibility of GBS conjugate vaccines. The authors previously developed a molecular serotype identification method based on serotype-specific PCR and partial sequencing of genes. In this study, a novel 10-primer pair multiplex PCR and reverse line blot (mPCR/RLB) hybridization assay was developed for simultaneous detection and serotype identification of all nine GBS serotypes. For all 316 GBS isolates tested the mPCR/RLB results corresponded with those of conventional serotyping and individual serotype-specific PCR, and the method was more convenient and practical than either alternative.

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2005-12-01
2019-11-14
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References

  1. Borchardt, S. M., Foxman, B., Chaffin, D. O., Rubens, C. E., Tallman, P. A., Manning, S. D., Baker, C. J. & Marrs, C. F. ( 2004;). Comparison of DNA dot blot hybridization and Lancefield capillary precipitin methods for group B streptococcal capsular typing. J Clin Microbiol 42, 146–150.[CrossRef]
    [Google Scholar]
  2. Cieslewicz, M. J., Chaffin, D., Glusman, G., Kasper, D., Madan, A., Rodrigues, S., Fahey, J., Wessels, M. R. & Rubens, C. E. ( 2005;). Structural and genetic diversity of group B streptococcus capsular polysaccharides. Infect Immun 73, 3096–3103.[CrossRef]
    [Google Scholar]
  3. Edwards, M. S., Rench, M. A., Palazzi, D. L. & Baker, C. J. ( 2005;). Group B streptococcal colonization and serotype-specific immunity in healthy elderly persons. Clin Infect Dis 40, 352–357.[CrossRef]
    [Google Scholar]
  4. Ekelund, K. & Konradsen, H. B. ( 2004;). Invasive group B streptococcal disease in infants, a 19-year nationwide study.Serotype distribution, incidence and recurrent infection. Epidemiol Infect 132, 1083–1090.[CrossRef]
    [Google Scholar]
  5. Elliott, J. A., Thompson, T. A., Facklam, R. R. & Slotved, H. C. ( 2004;). Increased sensitivity of a latex agglutination method for serotyping group B streptococcus. J Clin Microbiol 42, 3907. 3907.[CrossRef]
    [Google Scholar]
  6. Goguet de la Salmoniere, Y. O., Kim, C. C., Tsolaki, A. G., Pym, A. S., Siegrist, M. S. & Small, P. M. ( 2004;). High-throughput method for detecting genomic-deletion polymorphisms. J Clin Microbiol 42, 2913–2918.[CrossRef]
    [Google Scholar]
  7. Gold, B. ( 2003;). Origin and utility of the reverse dot-blot. Expert Rev Mol Diagn 3, 143–152.[CrossRef]
    [Google Scholar]
  8. Jones, N., Bohnsack, J. F., Takahashi, S. & 9 other authors ( 2003;). Multilocus sequence typing system for group B streptococcus. J Clin Microbiol 41, 2530–2536.[CrossRef]
    [Google Scholar]
  9. Ke, D. & Bergeron, M. G. ( 2001;). Molecular methods for rapid detection of group B streptococci. Expert Rev Mol Diagn 1, 175–181.[CrossRef]
    [Google Scholar]
  10. Kong, F., Gowan, S., Martin, D., James, G. & Gilbert, G. L. ( 2002;). Serotype identification of group B streptococci by PCR and sequencing. J Clin Microbiol 40, 216–226.[CrossRef]
    [Google Scholar]
  11. Kong, F., Martin, D., James, G. & Gilbert, G. L. ( 2003;). Towards a genotyping system for Streptococcus agalactiae (group B streptococcus): use of mobile genetic elements in Australasian invasive isolates. J Med Microbiol 52, 337–344.[CrossRef]
    [Google Scholar]
  12. Markoulatos, P., Siafakas, N. & Moncany, M. ( 2002;). Multiplex polymerase chain reaction: a practical approach. J Clin Lab Anal 16, 47–51.[CrossRef]
    [Google Scholar]
  13. Molano, M., Meijer, C. J., Morre, S. A., Pol, R. & van den Brule, A. J. ( 2004;). Combination of PCR targeting the VD2 of omp1 and reverse line blot analysis for typing of urogenital Chlamydia trachomatis serovars in cervical scrape specimens. J Clin Microbiol 42, 2935–2939.[CrossRef]
    [Google Scholar]
  14. Paoletti, L. C. & Madoff, L. C. ( 2002;). Vaccines to prevent neonatal GBS infection. Semin Neonatol 7, 315–323.[CrossRef]
    [Google Scholar]
  15. Schuchat, A. ( 1998;). Epidemiology of group B streptococcal disease in the United States: shifting paradigms. Clin Microbiol Rev 11, 497–513.
    [Google Scholar]
  16. Schuchat, A., Roome, A., Zell, E. R., Linardos, H., Zywicki, S. & O'Brien, K. L. ( 2002;). Integrated monitoring of a new group B streptococcal disease prevention program and other perinatal infections. Matern Child Health J 6, 107–114.[CrossRef]
    [Google Scholar]
  17. Tyrrell, G. J., Senzilet, L. D., Spika, J. S., Kertesz, D. A., Alagaratnam, M., Lovgren, M. & Talbot, J. A. ( 2000;). Invasive disease due to group B streptococcal infection in adults: results from a Canadian, population-based, active laboratory surveillance study – 1996.Sentinel Health Unit Surveillance System Site Coordinators. J Infect Dis 182, 168–173.[CrossRef]
    [Google Scholar]
  18. van den Brule, A. J., Pol, R., Fransen-Daalmeijer, N., Schouls, L. M., Meijer, C. J. & Snijders, P. J. ( 2002;). GP5+/6+ PCR followed by reverse line blot analysis enables rapid and high-throughput identification of human papillomavirus genotypes. J Clin Microbiol 40, 779–787.[CrossRef]
    [Google Scholar]
  19. Wang, H., Kong, F., Jelfs, P., James, G. & Gilbert, G. L. ( 2004;). Simultaneous detection and identification of common cell culture contaminant and pathogenic mollicutes strains by reverse line blot hybridization. Appl Environ Microbiol 70, 1483–1486.[CrossRef]
    [Google Scholar]
  20. Wittwer, C. T., Herrmann, M. G., Gundry, C. N. & Elenitoba-Johnson, K. S. ( 2001;). Real-time multiplex PCR assays. Methods 25, 430–442.[CrossRef]
    [Google Scholar]
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