1887

Abstract

Between January and April 2002, a total of 271 strains of were isolated from clinical specimens at Toho University Omori Hospital, Japan, including 201 (74·2 %) which were identified as meticillin-resistant (MRSA). However, 34 (12·5 %) were biochemically atypical, because they did not produce acid on mannitol salt agar or did not agglutinate in Staphaurex testing but were categorized as MRSA by PCR analysis and by antibiotic susceptibility. Three automatic identification systems, AutoScan-4 (Dade Behring), BD Phoenix (Becton Dickinson) and Vitek 2 (bioMérieux), were evaluated by testing these atypical isolates. The AutoScan-4 and Phoenix systems identified all 34 isolates as . Without additional tests such as Staphaurex, observation of colony pigment and haemolysins on sheep blood agar, Vitek 2 identified only 16 isolates (47·1 %) as with good or better confidence levels and misidentified one of the remaining isolates as . This study shows that it is possible to identify these physiologically atypical isolates correctly by using the Phoenix and AutoScan-4 fully automatic identification systems.

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2006-04-01
2019-10-22
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