%0 Journal Article %A Suffys, Philip %A da Silva Rocha, Adalgiza %A Brandão, Adeilton %A Vanderborght, Bart %A Mijs, Wouter %A Jannes, Geert %A Mello, Fernanda C. Q. %A Pedro, Heloisa da Silveira Paro %A de Souza Fonseca, Leila %A de Oliveira, Rosângela Siqueira %A Cardoso Leão, Sylvia %A Féres Saad, Maria Helena %T Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods %D 2006 %J Journal of Medical Microbiology, %V 55 %N 1 %P 127-131 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.46218-0 %K MAC, Mycobacterium avium complex %K ITS, internal transcribed spacer %K PRA, PCR–restriction enzyme analysis %K AFB, acid-fast bacillus %I Microbiology Society, %X Three mycobacterial isolates, one from the blood of an HIV-infected patient and two consecutive isolates from a woman with unknown HIV status, had been identified as belonging to the Mycobacterium avium complex by conventional procedures. In both patients, using genetic analysis procedures such as PCR–restriction enzyme analysis (PRA) of the hsp65 gene, a commercially available reverse hybridization-based assay (INNO-LiPA mycobacteria) and/or sequencing analysis of the 16S–23S internal transcribed spacer (ITS), the presence of Mycobacterium lentiflavum was also demonstrated. At the time of detection, both cases were also infected with M. avium, suggesting an underestimation of infection with M. lentiflavum and co-infection with different Mycobacterium species. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.46218-0