1887

Abstract

A total of 100 Jordanian clinical isolates was analysed for the presence of the enterotoxin genes , , , and using multiplex PCR. Twenty-three isolates (23 %) were potentially enterotoxigenic. The prevalence of , and plus among the total clinical isolates was 15, 4 and 4 %, respectively. None of the isolates harboured , or genes. isolates were subjected to DNA fingerprinting by randomly amplified polymorphic DNA (RAPD) analysis to test whether isolates harbouring the toxin genes were genetically clustered. A total of 13 genotypes was identified at a 47 % similarity level. Genotypes I and V accounted for the largest number of enterotoxigenic isolates (19 %). This study has demonstrated the genetic diversity of Jordanian clinical isolates and shown that the presence of the toxin genes is not genotype specific.

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2006-02-01
2019-10-18
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References

  1. Al-Zu'bi, E., Bdour, S. & Shehabi, A. A. ( 2004; ). Antibiotic resistance patterns of mecA-positive Staphylococcus aureus isolates from clinical specimens and nasal carriage. Microb Drug Resist 10, 321–324.[CrossRef]
    [Google Scholar]
  2. Araki, M., Kariyama, R., Monden, K., Tsugawa, M. & Kumon, H. ( 2002; ). Molecular epidemiological studies of Staphylococcus aureus in urinary tract infection. J Infect Chemother 8, 168–174.[CrossRef]
    [Google Scholar]
  3. Becker, K., Friedrich, A., Lubritz, G., Weilert, M., Peters, G. & von Eiff, C. ( 2003; ). Prevalence of genes encoding pyrogenic toxin superantigens and exofoliative toxins among strains of Staphylococcus aureus isolated from blood and nasal specimens. J Clin Microbiol 41, 1434–1439.[CrossRef]
    [Google Scholar]
  4. Byun, D. E., Kim, S. H., Shin, J. H., Suh, S. P. & Ryang, D. W. ( 1997; ). Molecular epidemiologic analysis of Staphylococcus aureus isolated from clinical specimens. J Korean Med Sci 12, 190–198.[CrossRef]
    [Google Scholar]
  5. Dinges, M. M., Orwin, P. M. & Schlievert, P. M. ( 2000; ). Exotoxins of Staphylococcus aureus. Clin Microbiol Rev 13, 16–34.[CrossRef]
    [Google Scholar]
  6. Frénay, H. M. E., Theelen, J. P. G., Schouls, L. M., Vandenbroucke-Grauls, C. M. J. E., Verhoef, J., van Leeuwen, W. J. & Mooi, F. R. ( 1994; ). Discrimination of epidemic and nonepidemic methicillin-resistant Staphylococcus aureus strains on the basis of protein A gene polymorphism. J Clin Microbiol 32, 846–847.
    [Google Scholar]
  7. Fueyo, J. M., Martin, M. C., Gonzalez-Hevia, M. A. & Mendoza, M. C. ( 2001; ). Enterotoxin production and DNA fingerprinting in Staphylococcus aureus isolated from human and food samples. Relations between genetic types and enterotoxins. Int J Food Microbiol 67, 139–145.[CrossRef]
    [Google Scholar]
  8. Jaccard, A. ( 1908; ). Nouvelles recherches sur la distribution florale. Bull Soc Vaudoises Sci Nat 44, 223–270 (in French).
    [Google Scholar]
  9. Jarraud, S., Peyrat, M. A., Lim, A. & 7 other authors ( 2001; ). egc, a highly prevalent operon of enterotoxin gene, forms a putative nursery of superantigens in Staphylococcus aureus. J Immunol 166, 669–677.[CrossRef]
    [Google Scholar]
  10. Kluytmans, J., van Leeuwen, W., Goessens, W. & 9 other authors ( 1995; ). Food initiated outbreak of methicillin-resistant Staphylococcus aureus analyzed by pheno- and genotyping. J Clin Microbiol 33, 1121–1128.
    [Google Scholar]
  11. Larsen, H. D., Aarestrup, F. M. & Jensen, N. E. ( 2002; ). Geographical variation in the presence of genes encoding superantigenic exotoxins and beta-hemolysin among Staphylococcus aureus isolated from bovine mastitis in Europe and USA. Vet Microbiol 85, 61–70.[CrossRef]
    [Google Scholar]
  12. Letertre, C., Perelle, S., Dilasser, F. & Fach, P. ( 2003; ). Identification of a new putative enterotoxin SEU encoded by the egc cluster of Staphylococcus aureus. J Appl Microbiol 95, 38–43.[CrossRef]
    [Google Scholar]
  13. Levin, R. I. & Rubin, D. S. ( 1991; ). Statistics for Management, 5th edn. Englewood Cliffs, NJ: Prentice Hall.
  14. McLauchlin, J., Narayanan, G. L., Mithani, V. & O'Neill, G. ( 2000; ). The detection of enterotoxins and toxic shock syndrome toxin genes in Staphylococcus aureus by polymerase chain reaction. J Food Prot 63, 479–488.
    [Google Scholar]
  15. Mehrotra, M., Wang, G. & Johnson, W. M. ( 2000; ). Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exofoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J Clin Microbiol 38, 1032–1035.
    [Google Scholar]
  16. Moore, P. C. L. & Lindsay, J. A. ( 2001; ). Genetic variation among hospital isolates of methicillin-sensitive Staphylococcus aureus: evidence for horizontal transfer of virulence genes. J Clin Microbiol 39, 2760–2767.[CrossRef]
    [Google Scholar]
  17. Omoe, K., Ishikawa, M., Shimoda, Y., Hu, D.-L., Ueda, S. & Shinagawa, K. ( 2002; ). Detection of seg, seh, and sei genes in Staphylococcus aureus isolates and determination of the enterotoxin productivities of S. aureus isolates harboring seg, seh, or sei genes. J Clin Microbiol 40, 857–862.[CrossRef]
    [Google Scholar]
  18. Rosec, J. P. & Gigaud, O. ( 2002; ). Staphylococcal enterotoxin genes of classical and new types detected by PCR in France. Int J Food Microbiol 77, 61–70.[CrossRef]
    [Google Scholar]
  19. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  20. Sharma, N. K., Rees, C. E. D. & Dodd, C. E. R. ( 2000; ). Development of a single reaction multiplex PCR toxin typing assay for Staphylococcus aureus strains. Appl Environ Microbiol 66, 1347–1353.[CrossRef]
    [Google Scholar]
  21. Su, Y.-C. & Wong, A. C. L. ( 1995; ). Identification and purification of a new staphylococcal enterotoxin, H. Appl Environ Microbiol 61, 1438–1443.
    [Google Scholar]
  22. Tambic, A., Power, E. G., Talsania, H., Antony, R. M. & French, G. L. ( 1997; ). Analysis of an outbreak of non-phage-typeable methicillin-resistant Staphylococcus aureus by using a randomly amplified polymorphic DNA assay. J Clin Microbiol 35, 3092–3097.
    [Google Scholar]
  23. Valle, J., Gomez-Lucia, E. & Piriz, S., Goyache, J., Orden, J. A. & Vadillo, S. ( 1990; ). Enterotoxin production by staphylococci isolated from healthy goats. Appl Environ Microbiol 56, 1323–1326.
    [Google Scholar]
  24. van Leeuwen, W. B., Jay, C., Snijders, S., Durin, N., Lacroix, B., Verbrugh, H. A., Enright, M. C., Troesch, A. & van Belkum, A. ( 2003; ). Multilocus sequence typing of Staphylococcus aureus with DNA array technology. J Clin Microbiol 41, 3323–3326.[CrossRef]
    [Google Scholar]
  25. Yoshida, T., Kondo, N., Abu-Hanifah, Y. & Hiramatsu, K. ( 1997; ). Combined use of ribotyping, PFGE typing and IS431 typing in the discrimination of nosocomial strains of methicillin-resistant Staphylococcus aureus. Microbiol Immunol 41, 687–695.[CrossRef]
    [Google Scholar]
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