1887

Abstract

The near full-length sequences of the genes were determined and analysed among eight reference strains (serotypes a to h) representing five species of mutans group streptococci. The sequences from these reference strains revealed that there are two lengths (285 and 288 bp) in the five species. The intergenic spacer between and appears to be a unique marker for species, with a variable size (ranging from 111 to 310 bp) and sequence. Phylogenetic analysis of and separated the eight serotypes into two major clusters. Strains of serotypes b, c, e and f were highly related and had gene sequences of the same length, 288 bp, while strains of serotypes a, d, g and h were also closely related and their gene sequence lengths were 285 bp. The sequences in clinical isolates of three serotypes of were analysed for intraspecies polymorphism. The results showed that the sequences could provide information for differentiation among species, but were unable to distinguish serotypes of the same species. Based on the determined sequences, a PCR assay was developed that could differentiate members of the mutans streptococci by amplicon size and provide an alternative way for distinguishing mutans streptococci from other viridans streptococci.

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2005-09-01
2019-11-22
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