1887

Abstract

The near full-length sequences of the genes were determined and analysed among eight reference strains (serotypes a to h) representing five species of mutans group streptococci. The sequences from these reference strains revealed that there are two lengths (285 and 288 bp) in the five species. The intergenic spacer between and appears to be a unique marker for species, with a variable size (ranging from 111 to 310 bp) and sequence. Phylogenetic analysis of and separated the eight serotypes into two major clusters. Strains of serotypes b, c, e and f were highly related and had gene sequences of the same length, 288 bp, while strains of serotypes a, d, g and h were also closely related and their gene sequence lengths were 285 bp. The sequences in clinical isolates of three serotypes of were analysed for intraspecies polymorphism. The results showed that the sequences could provide information for differentiation among species, but were unable to distinguish serotypes of the same species. Based on the determined sequences, a PCR assay was developed that could differentiate members of the mutans streptococci by amplicon size and provide an alternative way for distinguishing mutans streptococci from other viridans streptococci.

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2005-09-01
2024-12-08
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References

  1. Bukau B., Horwich A. L. 1998; The Hsp70 and Hsp60 chaperone machines. Cell 92:351–366 [CrossRef]
    [Google Scholar]
  2. Cvitkovitch D. G. 2001; Genetic competence and transformation in oral streptotocci. Crit Rev Oral Biol Med 12:217–243 [CrossRef]
    [Google Scholar]
  3. Facklam R. 2002; What happened to the streptococci: overview of taxonomic and nomenclature changes. Clin Microbiol Rev 15:613–630 [CrossRef]
    [Google Scholar]
  4. Goh S. H., Potter S., Wood J. O., Hemmingsen S. M., Reynolds R. P., Chow A. W. 1996; HSP60 gene sequences as universal targets for microbial species identification: studies with coagulase-negative staphylococci. J Clin Microbiol 34:818–823
    [Google Scholar]
  5. Goh S. H., Driedger D., Gillett S. & 8 other authors; 1998; Streptococcus iniae , a human and animal pathogen: specific identification by the chaperonin 60 gene identification method. J Clin Microbiol 36:2164–2166
    [Google Scholar]
  6. Goh S. H., Facklam R. R., Chang M. & 8 other authors; 2000; Identification of Enterococcus species and phenotypically similar Lactococcus and Vagococcus species by reverse checkerboard hybridization to chaperonin 60 gene sequences. J Clin Microbiol 38:3953–3959
    [Google Scholar]
  7. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  8. Oho T., Yamashita Y., Shimazaki Y., Kushiyama M., Koga T. 2000; Simple and rapid detection of Streptococcus mutans and Streptococcus sobrinus in human saliva by polymerase chain reaction. Oral Microbiol Immunol 15:258–262 [CrossRef]
    [Google Scholar]
  9. Sato T., Hu J. P., Ohki K., Yamaura M., Washio J., Matsuyama J., Takahashi N. 2003; Identification of mutans streptococci restriction fragment length polymorphism analysis of polymerase chain reaction-amplified 16S ribosomal RNA genes. Oral Microbiol Immunol 18:323–326 [CrossRef]
    [Google Scholar]
  10. Shibata Y., Ozaki K., Seki M., Kawato T., Tanaka H., Nakano Y., Yamashita Y. 2003; Analysis of loci required for determination of serotype antigenicity in Streptococcus mutans and its clinical utilization. J Clin Microbiol 41:4107–4112 [CrossRef]
    [Google Scholar]
  11. Teng L. J., Hsueh P. R., Wang Y. H., Lin H. M., Luh K. T., Ho S. W. 2001; Determination of Enterococcus faecalis groESL full-length sequence and application for species identification. J Clin Microbiol 39:3326–3331 [CrossRef]
    [Google Scholar]
  12. Teng L. J., Hsueh P. R., Tsai J. C., Chen P. W., Hsu J. C., Lai H. C., Lee C. N., Ho S. W. 2002; groESL sequence determination, phylogenetic analysis, and species differentiation for viridans group streptococci. J Clin Microbiol 40:3172–3178 [CrossRef]
    [Google Scholar]
  13. Truong T. L., Menard C., Mouton C., Trahan L. 2000; Identification of mutans and other oral streptococci by random amplified polymorphic DNA analysis. J Med Microbiol 49:63–71
    [Google Scholar]
  14. Tsai J. C., Hsueh P. R., Lin H. M., Chang H. J., Ho S. W., Teng L. J. 2005; Identification of clinically relevant Enterococcus species by direct sequencing of groES and spacer region. J Clin Microbiol 43:235–241 [CrossRef]
    [Google Scholar]
  15. Whiley R. A., Beighton D. 1998; Current classification of the oral streptococci. Oral Microbiol Immunol 13:195–216 [CrossRef]
    [Google Scholar]
  16. Yoshida A., Suzuki N., Nakano Y., Kawada M., Oho T., Koga T. 2003; Development of a 5′ nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus . J Clin Microbiol 41:4438–4441 [CrossRef]
    [Google Scholar]
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