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Volume 55, Issue 1

Distribution and genomic location of active insertion sequences in the complex Free

Abstract

This study aimed firstly to establish the distribution and copy number within the complex of three insertion sequences (IS, IS and IS) that possess the ability to activate transcription and hence influence gene expression. A second aim was to map the genomic insertion sites of one of the active insertion sequences (IS) to establish putative links between insertion site and downstream gene activation. The resulting data revealed that all three insertion sequences were present in one-third of the 66 isolates tested. The three insertion sequences were prevalent across the nine complex species, although IS was absent from the 16 strains tested and IS was absent from all 10 strains. IS copies from six strains (two strains and one strain each of , , and ) were mapped to the genome using hemi-nested inverse PCR. Insertions were found upstream of genes with wide-ranging functions. This study suggests that the abundance and distribution of these active insertion sequences is likely to affect genomic plasticity, and potentially gene transcription and pathogenicity.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bcc, Burkholderia cepacia complex , CF, cystic fibrosis , hINVPCR, hemi-nested inverse PCR and IS, insertion sequence
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2006-01-01
2024-04-25
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Distribution and genomic location of active insertion sequences in the Burkholderia cepacia complex
J. Med. Microbiol. 55, 1 (2006); https://doi.org/10.1099/jmm.0.46175-0
/content/journal/jmm/10.1099/jmm.0.46175-0
/content/journal/jmm/10.1099/jmm.0.46175-0
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