1887

Abstract

A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified genes with 100 % accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.

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2005-12-01
2019-10-18
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References

  1. Alobwede, I., M'Zali, F. H., Livermore, D. M., Heritage, J., Todd, N. & Hawkey, P. M. ( 2003;). CTX-M extended-spectrum beta-lactamase arrives in the UK. J Antimicrob Chemother 51, 470–471.[CrossRef]
    [Google Scholar]
  2. Arpin, C., Dubois, V., Coulange, L. & 10 other authors ( 2003;). Extended-spectrum beta-lactamase-producing Enterobacteriaceae in community and private health care centers. Antimicrob Agents Chemother 47, 3506–3514.[CrossRef]
    [Google Scholar]
  3. Baraniak, A., Fiett, J., Sulikowska, A., Hryniewicz, W. & Gniadkowski, M. ( 2002;). Countrywide spread of CTX-M-3 extended-spectrum beta-lactamase-producing microorganisms of the family Enterobacteriaceae in Poland. Antimicrob Agents Chemother 46, 151–159.[CrossRef]
    [Google Scholar]
  4. Bonnet, R. ( 2004;). Growing group of extended-spectrum beta- lactamases: the CTX-M enzymes. Antimicrob Agents Chemother 48, 1–14.[CrossRef]
    [Google Scholar]
  5. Bonnet, R., Sampaio, J. L., Labia, R., De Champs, C., Sirot, D., Chanal, C. & Sirot, J. ( 2000;). A novel CTX-M beta-lactamase (CTX-M-8) in cefotaxime-resistant Enterobacteriaceae isolated in Brazil. Antimicrob Agents Chemother 44, 1936–1942.[CrossRef]
    [Google Scholar]
  6. Boyd, D. A., Tyler, S., Christianson, S. & 7 other authors ( 2004;). Complete nucleotide sequence of a 92-kilobase plasmid harboring the CTX-M-15 extended-spectrum beta-lactamase involved in an outbreak in long-term-care facilities in Toronto, Canada. Antimicrob Agents Chemother 48, 3758–3764.[CrossRef]
    [Google Scholar]
  7. Brenwald, N. P., Jevons, G., Andrews, J. M., Xiong, J. H., Hawkey, P. M. & Wise, R. ( 2003;). An outbreak of a CTX-M-type beta-lactamase-producing Klebsiella pneumoniae: the importance of using cefpodoxime to detect extended-spectrum beta-lactamases. J Antimicrob Chemother 51, 195–196.[CrossRef]
    [Google Scholar]
  8. Canton, R., Oliver, A., Coque, T. M., Varela, M. C., Perez-Diaz, J. C. & Baquero, F. ( 2002;). Epidemiology of extended-spectrum beta- lactamase-producing Enterobacter isolates in a Spanish hospital during a 12-year period. J Clin Microbiol 40, 1237–1243.[CrossRef]
    [Google Scholar]
  9. Cao, V., Lambert, T. & Courvalin, P. ( 2002;). ColE1-like plasmid pIP843 of Klebsiella pneumoniae encoding extended-spectrum beta-lactamase CTX-M-17. Antimicrob Agents Chemother 46, 1212–1217.[CrossRef]
    [Google Scholar]
  10. Chanawong, A., M'Zali, F. H., Heritage, J., Xiong, J. H. & Hawkey, P. M. ( 2002;). Three cefotaximases, CTX-M-9, CTX-M-13, and CTX-M-14, among Enterobacteriaceae in the People's Republic of China. Antimicrob Agents Chemother 46, 630–637.[CrossRef]
    [Google Scholar]
  11. Colom, K., Perez, J., Alonso, R., Fernandez-Aranguiz, A., Larino, E. & Cisterna, R. ( 2003;). Simple and reliable multiplex PCR assay for detection of blaTEM, bla(SHV) and blaOXA-1 genes in Enterobacteriaceae. FEMS Microbiol Lett 223, 147–151.[CrossRef]
    [Google Scholar]
  12. Cooksey, R. C., Morlock, G. P., Holloway, B. P., Limor, J. & Hepburn, M. ( 2002;). Temperature-mediated heteroduplex analysis performed by using denaturing high-performance liquid chromatography to identify sequence polymorphisms in Mycobacterium tuberculosis complex organisms. J Clin Microbiol 40, 1610–1616.[CrossRef]
    [Google Scholar]
  13. Eaves, D. J., Liebana, E., Woodward, M. J. & Piddock, L. J. ( 2002;). Detection of gyrA mutations in quinolone-resistant Salmonella enterica by denaturing high-performance liquid chromatography. J Clin Microbiol 40, 4121–4125.[CrossRef]
    [Google Scholar]
  14. Evans, J. T., Hawkey, P. M., Smith, E. G., Boese, K. A., Warren, R. E. & Hong, G. ( 2004;). Automated high-throughput mycobacterial interspersed repetitive unit typing of Mycobacterium tuberculosis strains by a combination of PCR and nondenaturing high-performance liquid chromatography. J Clin Microbiol 42, 4175–4180.[CrossRef]
    [Google Scholar]
  15. Hurtle, W., Bode, E., Kaplan, R. S., Garrison, J., Kearney, B., Shoemaker, D., Henchal, E. & Norwood, D. ( 2003;). Use of denaturing high-performance liquid chromatography to identify Bacillus anthracis by analysis of the 16S-23S rRNA interspacer region and gyrA gene. J Clin Microbiol 41, 4758–4766.[CrossRef]
    [Google Scholar]
  16. Karim, A., Poirel, L., Nagarajan, S. & Nordmann, P. ( 2001;). Plasmid-mediated extended-spectrum beta-lactamase (CTX-M-3 like) from India and gene association with insertion sequence ISEcp1. FEMS Microbiol Lett 201, 237–241.
    [Google Scholar]
  17. Moland, E. S., Black, J. A., Hossain, A., Hanson, N. D., Thomson, K. S. & Pottumarthy, S. ( 2003;). Discovery of CTX-M-like extended-spectrum beta-lactamases in Escherichia coli isolates from five US states. Antimicrob Agents Chemother 47, 2382–2383.[CrossRef]
    [Google Scholar]
  18. Munday, C. J., Whitehead, G. M., Todd, N. J., Campbell, M. & Hawkey, P. M. ( 2004;). Predominance and genetic diversity of community- and hospital-acquired CTX-M extended-spectrum beta-lactamases in York, UK. J Antimicrob Chemother 54, 628–633.[CrossRef]
    [Google Scholar]
  19. Pagani, L., Dell'Amico, E., Migliavacca, R., D'Andrea, M. M., Giacobone, E., Amicosante, G., Romero, E. & Rossolini, G. M. ( 2003;). Multiple CTX-M-type extended-spectrum beta-lactamases in nosocomial isolates of Enterobacteriaceae from a hospital in northern Italy. J Clin Microbiol 41, 4264–4269.[CrossRef]
    [Google Scholar]
  20. Perez-Perez, F. J. & Hanson, N. D. ( 2002;). Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. J Clin Microbiol 40, 2153–2162.[CrossRef]
    [Google Scholar]
  21. Pitout, J. D., Hossain, A. & Hanson, N. D. ( 2004;). Phenotypic and molecular detection of CTX-M-beta-lactamases produced by Escherichia coli and Klebsiella spp. J Clin Microbiol 42, 5715–5721.[CrossRef]
    [Google Scholar]
  22. Saladin, M., Cao, V. T., Lambert, T. & 7 other authors ( 2002;). Diversity of CTX-M beta-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals. FEMS Microbiol Lett 209, 161–168.
    [Google Scholar]
  23. Tzouvelekis, L. S., Tzelepi, E., Tassios, P. T. & Legakis, N. J. ( 2000;). CTX-M-type beta-lactamases: an emerging group of extended- spectrum enzymes. Int J Antimicrob Agents 14, 137–142.[CrossRef]
    [Google Scholar]
  24. Winstanley, T. G., Ridgway, E. J., Parys, B. T., Woodford, N., Ward, E. & Livermore, D. M. ( 2004;). First isolation of a CTX-M-3 beta-lactamase producer in the United Kingdom. Int J Antimicrob Agents 24, 625–627.[CrossRef]
    [Google Scholar]
  25. Woodford, N., Ward, M. E., Kaufmann, M. E. & 14 other authors ( 2004;). Community and hospital spread of Escherichia coli producing CTX-M extended-spectrum beta-lactamases in the UK. J Antimicrob Chemother 54, 735–743.[CrossRef]
    [Google Scholar]
  26. Woodford, N., Fagan, E. J. & Ellington, M. J. ( 2005;). Development of multiplex PCR assay for genes encoding CTX-M extended-spectrum beta-lactamases. Clin Microbiol Infect 11 (Suppl. s2), 121. 121.
    [Google Scholar]
  27. Yan, J. J., Ko, W. C., Tsai, S. H., Wu, H. M., Jin, Y. T. & Wu, J. J. ( 2000;). Dissemination of CTX-M-3 and CMY-2 beta-lactamases among clinical isolates of Escherichia coli in southern Taiwan. J Clin Microbiol 38, 4320–4325.
    [Google Scholar]
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