1887

Abstract

In the present study, three strains and one strain out of a collection of 241 isolates from human and food-processing sources were found to display resistance to tetracycline (TC) due to the presence of the (M) gene. Through sequence analysis, it was shown that (M) genes in two of the isolates belong to sequence homology group (SHG) II, a group comprising chromosomally encoded (M) genes previously found in and in lactobacilli. The (M) genes of the two other strains were associated with a member of the Tn–Tn family of conjugative transposons and were closely related to SHG III, which harbours enterococcal (M) genes associated with Tn. One of these transposon-containing strains was able to transfer the (M) gene to recipient strain JH2-2. Collectively, these sequence and conjugation data indicate that the acquisition of (M) by strains may be triggered by successive transfers between other Gram-positive organisms.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46142-0
2005-12-01
2019-11-20
Loading full text...

Full text loading...

/deliver/fulltext/jmm/54/12/JM541206.html?itemId=/content/journal/jmm/10.1099/jmm.0.46142-0&mimeType=html&fmt=ahah

References

  1. Anderson, D. G. & McKay, L. L. ( 1983;). Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol 46, 549–552.
    [Google Scholar]
  2. Antunes, P., Réu, C., Sousa, J. C., Pestana, N. & Peixe, L. ( 2002;). Incidence and susceptibility to antimicrobial agents of Listeria spp.and Listeria monocytogenes isolated from poultry carcasses in Porto, Portugal. J Food Prot 65, 1888–1893.
    [Google Scholar]
  3. Charpentier, E. & Courvalin, P. ( 1999;). Antibiotic resistance in Listeria spp. Antimicrob Agents Chemother 43, 2103–2108.
    [Google Scholar]
  4. Charpentier, E., Gerbaux, G., Jacquet, C., Rocourt, J. & Courvalin, P. ( 1995;). Incidence of antibiotic resistance in Listeria species. J Infect Dis 172, 277–281.[CrossRef]
    [Google Scholar]
  5. Clermont, D., Chesneau, O., De Cespédès, G. & Horaud, T. ( 1997;). New tetracycline resistance determinants coding for ribosomal protection in Streptococci and nucleotide sequence of tet(T) isolated from Streptococcus pyogenes A498. Antimicrob Agents Chemother 41, 112–116.
    [Google Scholar]
  6. Clewell, D. B., Flannagan, S. E. & Jaworski, D. D. ( 1995;). Unconstrained bacterial promiscuity: the Tn916-Tn1545 family of conjugative transposons. Trends Microbiol 3, 229–236.[CrossRef]
    [Google Scholar]
  7. Connell, S. R., Tracz, D. M., Nierhaus, K. H. & Taylor, D. E. ( 2003;). Ribosomal protection proteins and their mechanism of tetracycline resistance. Antimicrob Agents Chemother 47, 3675–3681.[CrossRef]
    [Google Scholar]
  8. Doherty, N., Trzcinski, K., Pickerill, P., Zawadzki, P. & Dowson, C. ( 2000;). Genetic diversity of the tet(M) gene in tetracycline-resistant clonal lineages of Streptococcus pneumoniae. Antimicrob Agents Chemother 44, 2979–2984.[CrossRef]
    [Google Scholar]
  9. Doucet-Populaire, F., Trieu-Cuot, P., Dosbaa, I., Andremont, A. & Courvalin, P. ( 1991;). Inducible transfer of conjugative transposon Tn1545 from Enterococcus faecalis to Listeria monocytogenes in the digestive tracts of gnotobiotic mice. Antimicrob Agents Chemother 35, 185–187.[CrossRef]
    [Google Scholar]
  10. Engel, H. W., Soedirman, N., Rost, J. A., van Leeuwen, W. J. & van Embden, J. D. ( 1980;). Transferability of macrolide, lincomycin, and streptogramin resistances between groups A, B, and D streptococci, Streptococcus pneumoniae, and Staphylococcus aureus. J Bacteriol 142, 407–413.
    [Google Scholar]
  11. Facinelli, B., Roberts, M. C., Giovanetti, E., Casolari, C., Fabio, U. & Varaldo, P. E. ( 1993;). Genetic basis of tetracycline resistance in food-borne isolates of Listeria innocua. Appl Environ Microbiol 59, 614–616.
    [Google Scholar]
  12. Gevers, D., Danielsen, M., Huys, G. & Swings, J. ( 2003;). Molecular characterization of tet(M) genes in Lactobacillus isolates from different types of fermented dry sausage. Appl Environ Microbiol 69, 1270–1275.[CrossRef]
    [Google Scholar]
  13. Guardabassi, L., Dijkshoorn, L., Collard, J.-M., Olsen, J. E. & Dalsgaard, A. ( 2000;). Distribution and in-vitro transfer of tetracycline resistance determinants in clinical and aquatic Acinetobacter strains. J Med Microbiol 49, 929–936.
    [Google Scholar]
  14. Guillaume, G., Ledent, V., Moens, W. & Collard, J. M. ( 2004;). Phylogeny of efflux-mediated tetracycline resistance genes and related proteins revisited. Microb Drug Resist 10, 11–26.[CrossRef]
    [Google Scholar]
  15. Hof, H., Nichterlein, T. & Kretschmar, M. ( 1997;). Management of listeriosis. Clin Microbiol Rev 10, 345–357.
    [Google Scholar]
  16. Huys, G., D'Haene, K., Collard, J.-M. & Swings, J. ( 2004;). Prevalence and molecular characterization of tetracycline resistance in Enterococcus isolates from food. Appl Environ Microbiol 70, 1555–1562.[CrossRef]
    [Google Scholar]
  17. Jacob, A. E. & Hobbs, S. J. ( 1974;). Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var.zymogenes. J Bacteriol 117, 360–372.
    [Google Scholar]
  18. Margolles, A. & de los Reyes-Gavilan, C. G. D. ( 1998;). Characterization of plasmids from Listeria monocytogenes and Listeria innocua strains isolated from short-ripened cheeses. Int J Food Microbiol 39, 231–236.[CrossRef]
    [Google Scholar]
  19. Marra, D., Pethel, B., Churchward, G. G. & Scott, J. R. ( 1999;). The frequency of conjugative transposition of Tn916 is not determined by the frequency of excision. J Bacteriol 181, 5414–5418.
    [Google Scholar]
  20. Morse, S. A., Johnson, S. R., Biddle, J. W., Roberts, M. C. ( 1986;). High-level tetracycline resistance in Neisseria gonorrhoeae is result of acquisition of streptococcal tetM determinant. Antimicrob Agents Chemother 30, 664–670.[CrossRef]
    [Google Scholar]
  21. Perreten, V., Kollofel, B. & Teuber, M. ( 1997a;). Conjugal transfer of the Tn916-like transposon TnFO1 from Enterococcus faecalis isolated from cheese to other Gram-positive bacteria. Syst Appl Microbiol 20, 27–38.[CrossRef]
    [Google Scholar]
  22. Perreten, V., Schwartz, F., Cresta, L., Boeglin, M., Dasen, G. & Teuber, M. ( 1997b;). Antibiotic resistance spread in food. Nature 389, 801–802.
    [Google Scholar]
  23. Pourshaban, M., Ferrini, A. M., Mannoni, V., Oliva, B. & Aureli, P. ( 2002;). Transferable tetracycline resistance in Listeria monocytogenes from food in Italy. J Med Microbiol 51, 564–566.
    [Google Scholar]
  24. Poyart-Salmeron, C., Carlier, C., Trieu-Cuot, P., Courtieu, A. L. & Courvalin, P. ( 1990;). Transferable plasmid-mediated antibiotic resistance in Listeria monocytogenes. Lancet 335, 1422–1426.[CrossRef]
    [Google Scholar]
  25. Roberts, M. ( 2004;). Mechanism of resistance for characterized tet and otr genes. Last accessed 6 May 2004. http://faculty.washington.edu/marilynr/tetweb1.pdf
  26. Rocourt, J. ( 1996;). Taxonomie du genre Listeria et typage de L.monocytogenes. Pathol Biol 44, 749–756 (in French).
    [Google Scholar]
  27. Rocourt, J. & Cossart, P. ( 1997;). Food Microbiology: Fundamentals and Frontiers. Washington, DC: American Society for Microbiology.
  28. Safdar, A. & Armstrong, D. ( 2003;). Antimicrobial activities against 84 Listeria monocytogenes isolates from patients with systemic listeriosis at a comprehensive cancer center (1955–1997). J Clin Microbiol 41, 483–485.[CrossRef]
    [Google Scholar]
  29. Schwarz, S., Cardoso, M. & Wegener, H. C. ( 1992;). Nucleotide sequence and phylogeny of the tet(L) tetracycline resistance determinant encoded by plasmid pSTE1 from Staphylococcus hyicus. Antimicrob Agents Chemother 36, 580–588.[CrossRef]
    [Google Scholar]
  30. Soussy, C. J. ( 2005;). Communiqué 2005. Comité de l'Antibiogramme de la Société Française de Microbiologie. Paris: Société Française de Microbiologie (in French). http://www.sfm.asso.fr/
  31. Swann, M. ( 1969;). Report of the Joint Committee on the Use of Antibiotics in Animal Husbandry and Veterinary Medicine. London: Her Majesty's Stationery Office.
  32. Wellington, E. M. H. ( 2000;). Antibiotic resistance genes in the environment: a comprehensive, multi-phasic survey of prevalence and transfer. EU Research Project, Contract number BIO4-CT98-0053. http://europa.eu.int/comm/research/quality-of-life/gmo/06-tools/06-13-project.html
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46142-0
Loading
/content/journal/jmm/10.1099/jmm.0.46142-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error