1887

Journal of Medical Microbiology logo

Volume 55, Issue 2

Experimental model of infection with non-toxigenic strains of and development of septic arthritis Free

Abstract

is a well-known cause of localized respiratory tract infections. However, this micro-organism can also be associated with invasive infections, such as endocarditis, septic arthritis and osteomyelitis. Invasive infections are often caused by non-toxigenic strains. To set up an experimental model of infection, mice were infected intravenously with different doses (ranging from 1×10 to 5×10 bacteria per mouse) of three non-toxigenic strains, namely ISS-4749, ISS-4746 and ISS-3319. Similar mortality rates were observed with the three strains, with an LD ranging from 9×10 to 1·2×10. All strains were arthritogenic, although to different extents. ISS-4749 and ISS-4746 infection resulted in a maximum of 60 and 50 %, respectively, of animals with articular lesions, while in the ISS-3319-infected group only 25 % were positive. There were differences in systemic and joint cytokine production in the three experimental groups. ISS-4749- and ISS-4746-infected mice exhibited higher local levels of interleukin (IL)-6 and IL-1 than ISS-3319-infected animals. At systemic levels, ISS-3319 was able to induce early and sustained production of interferon- (IFN-), but not IL-6. Conversely, infection with the other strains resulted in high IL-6, but not IFN-, production. In conclusion, an experimental model of infection was set up, with development of septic arthritis. This model could be useful in studies on the pathogenicity and characterization of virulence factors other than toxin production.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): IFN, interferon , IL, interleukin and TNF, tumour necrosis factor
SGM
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46135-0
2006-02-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/2/229.html?itemId=/content/journal/jmm/10.1099/jmm.0.46135-0&mimeType=html&fmt=ahah

References

  1. Afghani B., Stutman H. A. 1993; Bacterial arthritis caused by Corynebacterium diphtheriae . Pediatr Infect Dis J 12:881–882 [CrossRef]
     [Google Scholar]
  2. Alexander D. 1984; Splenic abscess caused by Corynebacterium diphtheriae . Clin Pediatr 23:591–592 [CrossRef]
     [Google Scholar]
  3. Appelbaum P. C., Dossett J. H. 1982; Arthritis caused by unidentified gram-positive rods in a child with acute lymphocytic leukemia. J Clin Microbiol 15:525–527
     [Google Scholar]
  4. Barakett V., Morel G., Lesage D., Petit J. C. 1993; Septic arthritis due to a non-toxigenic strain of Corynebacterium diphtheriae subspecies mitis . Clin Infect Dis 17:520–521 [CrossRef]
     [Google Scholar]
  5. Belko J., Wessel D. L., Malley R. 2000; Endocarditis caused by Corynebacterium diphtheriae : case report and review of the literature. Pediatr Infect Dis J 19:159–163 [CrossRef]
     [Google Scholar]
  6. Bertuccini L., Baldassarri L., von Hunolstein C. 2004; Internalization of non-toxigenic Corynebacterium diphtheriae by cultured human respiratory epithelial cells. Microb Pathog 37:111–118 [CrossRef]
     [Google Scholar]
  7. Damade R., Pouchot J., Delacroix I., Boussougant Y., Vinceneux P. 1993; Septic arthritis due to Corynebacterium diphtheriae . Clin Infect Dis 16:446–447 [CrossRef]
     [Google Scholar]
  8. de Oliveira Moreira L., Andrade A. F. B., Vale M. D. & 7 other authors; 2003; Effects of iron limitation on adherence and cell surface carbohydrates of Corynebacterium diphtheriae strains. Appl Environ Microbiol 69:5907–5913 [CrossRef]
     [Google Scholar]
  9. Funke G., Altwegg M., Frommel L., von Graevenitz A. A. 1999; Emergence of related nontoxigenic Corynebacterium diphtheriae biotype mitis strains in Western Europe. Emerg Infect Dis 5:477–480 [CrossRef]
     [Google Scholar]
  10. Fuseler J. W., Conner E. M., Davis J. M., Wolf R. E., Grisham M. V. 1997; Cytokine and nitric oxide production in the acute phase of bacterial cell wall-induced arthritis. Inflammation 21:113–131 [CrossRef]
     [Google Scholar]
  11. Galazka A. 2000; The changing epidemiology of diphtheria in the vaccine era. J Infect Dis 181 (Suppl. 1):S2–S9 [CrossRef]
     [Google Scholar]
  12. Green J., Schotland S., Sella Z., Kleeman C. R. 1994; Interleukin-6 attenuates agonist-mediated calcium mobilization in murine osteoblastic cells. J Clin Invest 93:2340–2350 [CrossRef]
     [Google Scholar]
  13. Guran P., Mollaret H. H., Chatelain R., Gropman M., Prigent F., Beal G. 1979; Arthrite purulent à bacille diphtérique atoxinogène. Arch Fr Pediatr 36:926–929 (in French
    [Google Scholar]
  14. Ito A., Itoh Y., Sasaguri Y., Morimatsu M., Mori Y. 1992; Effects of interleukin-6 on the metabolism of the connective tissue components in rheumatoid synovial fibroblasts. Arthritis Rheum 35:1197–1201 [CrossRef]
     [Google Scholar]
  15. Josefsson E., Hartford O., O'Brien L., Patti J. M., Foster T. 2001; Protection against experimental Staphylococcus aureus arthritis by vaccination with clumping factor A, a novel virulence determinant. J Infect Dis 184:1572–1580 [CrossRef]
     [Google Scholar]
  16. Mattos-Guaraldi A. L., Formiga L. C. D. 1998; Bacteriological properties of a sucrose-fermenting Corynebacterium diphtheriae strain isolated from a case of endocarditis. Curr Microbiol 37:156–158 [CrossRef]
     [Google Scholar]
  17. Mattos-Guaraldi A. L., Formiga L. C. D., Pereira G. A. 2000; Cell surface components and adhesion in Corynebacterium diphtheriae . Microbes Infect 2:1507–1512 [CrossRef]
     [Google Scholar]
  18. Mattos-Guaraldi A. L., Formiga L. C., Camello T. C., Pereira G. A., Hirata R. Jr, Halpern M. 2001; Corynebacterium diphtheriae threats in cancer patients. Rev Argent Microbiol 33:96–100
     [Google Scholar]
  19. Nietfeld J. J., Wilbrink B., Helle M., van Roy J. L., den Otter W., Swaak A. J., Huber-Bruning O. 1990; Interleukin-1-induced interleukin-6 is required for the inhibition of proteoglycan synthesis by interleukin-1 in human articular cartilage. Arthritis Rheum 33:1695–1701 [CrossRef]
     [Google Scholar]
  20. Nilsson I. M., Hartford O., Foster T., Tarkowski A. 1999; Alpha-toxin and gamma-toxin jointly promote Staphylococcus aureus virulence in murine septic arthritis. Infect Immun 67:1045–1049
     [Google Scholar]
  21. Osiri M., Ruxrungtham K., Nookhai S., Ohmoto Y., Deesomchok U. 1998; IL-1 β , IL-6 and TNF- α in synovial fluid of patients with non-gonococcal septic arthritis. Asian Pac J Allergy Immunol 16:155–160
     [Google Scholar]
  22. Patey O., Bimet F., Riegel P. & 8 other authors; 1997; Clinical and molecular study of Corynebacterium diphtheriae systemic infections in France. J Clin Microbiol 35:441–445
     [Google Scholar]
  23. Poilane I., Fawaz F., Nathanson M., Cruaud P., Martin T., Collignon A., Gaudelus J. 1995; Corynebacterium diphtheriae osteomyelitis in an immunocompetent child: a case report. Eur J Pediatr 154:381–383 [CrossRef]
     [Google Scholar]
  24. Puliti M., von Hunolstein C., Bistoni F., Mosci P., Orefici G., Tissi L. 2000; Influence of interferon- γ administration in the severity of experimental group B streptococcal arthritis. Arthritis Rheum 43:2678–2696 [CrossRef]
     [Google Scholar]
  25. Saragea A., Maximescu P., Meiter E. 1979; Microbiological methods used in clinical and epidemiological investigations. Methods Microbiol 13:62–176
     [Google Scholar]
  26. Tarkowski A., Bokarewa M., Collins L. V. & 7 other authors; 2002; Current status of pathogenetic mechanisms in staphylococcal arthritis. FEMS Microbiol Lett 217:125–132 [CrossRef]
     [Google Scholar]
  27. Tiley S. M., Kociuba K. R., Heron L. G., Munro R. 1993; Infective endocarditis due to nontoxigenic Corynebacterium diphtheriae : report of seven cases and review. Clin Infect Dis 16:271–275 [CrossRef]
     [Google Scholar]
  28. Tissi L., Puliti M., Barluzzi R., Orefici G., von Hunolstein C., Bistoni F. 1999; Role of tumor necrosis factor alpha, interleukin-1 β , and interleukin-6 in a mouse model of group B streptococcal arthritis. Infect Immun 67:4545–4550
     [Google Scholar]
  29. Verdrengh M., Tarkowski A. 1997; Role of neutrophils in experimental septicemia and septic arthritis induced by Staphylococcus aureus . Infect Immun 65:2517–2521
     [Google Scholar]
  30. von Hunolstein C., Alfarone G., Scopetti F. & 10 other authors; 2003; Molecular epidemiology and characteristics of Corynebacterium diphtheriae and Corynebacterium ulcerans strains isolated in Italy during the 1990s. J Med Microbiol 52:181–188 [CrossRef]
     [Google Scholar]
  31. Wilson A. P. R. T. 1995; The return of Corynebacterium diphtheriae : the rise of non-toxigenic strains. J Hosp Infect 30:Suppl.306–312 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46135-0
Loading
Experimental model of infection with non-toxigenic strains of Corynebacterium diphtheriae and development of septic arthritis
J. Med. Microbiol. 55, 229 (2006); https://doi.org/10.1099/jmm.0.46135-0
/content/journal/jmm/10.1099/jmm.0.46135-0
/content/journal/jmm/10.1099/jmm.0.46135-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error