1887

Abstract

Seroepidemiological studies have indicated that infection might be a possible risk factor for colorectal adenocarcinoma (CRC) development. However, limited information is available as to whether or not species are present in CRC tissues. In this study the presence of DNA in 77 CRC biopsies was investigated by means of a species-specific 16S rDNA PCR assay and real-time DNA pyrosequencing of the 16S rDNA variable V3 region. Pyrosequencing revealed the presence of DNA sequences in 21 of 77 biopsy specimens (27 %). 16S rDNA sequences corresponding to 26695 and J99 were most commonly found. Intriguingly, one sequence belonged to , previously identified in ferrets. No significant correlations were found in the prevalence of DNA between colon and rectum tumour biopsies ( = 0.815), nor between Dukes’ classes A/B and C/D ( = 0.262). 16S rDNA PCR amplification combined with pyrosequencing analysis of 16S rDNA variable V3 regions provides a powerful molecular tool to identify species in human biopsy specimens.

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2005-11-01
2019-12-06
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