Evaluation of commercial kits for the identification of Free

Abstract

Eight identification methods were evaluated against 100 isolates of and 21 non-gonococcal strains. The methods examined included four commercial biochemical kits, API NH, RapID NH, Gonochek II and Preformed Enzyme Test (PET), three immunological kits, Phadebact Monoclonal GC test, GonoGen II and MicroTrak, and one in-house carbohydrate-utilization method, cystine trypticase agar (CTA) sugars. The percentage of isolates unambiguously identified as by each of the methods was as follows: API NH, 66 %; RapID NH, 64 %; GonoChek II, 66 %; PET, 66 %; Phadebact Monoclonal GC OMNI test, 99 %; GonoGen II, 100 %; MicroTrak, 100 %; and CTA sugars, 96 %. The low sensitivity of the biochemical kits for the identification of was due to a lack of the enzyme proline iminopeptidase (Pip) in 34 % of the isolates examined. All the biochemical kits utilized the presence of this enzyme as a marker for . The Phadebact Monoclonal GC kit, GonoGen II, MicroTrak, CTA sugars and the API NH kit all exhibited high specificity, but non-gonococcal were misidentified as using RapID NH (two strains), Gonochek II (11 strains) and PET (11 strains). Whilst the isolates examined in this study may not be truly representative, they do indicate that isolates lacking the enzyme Pip can give anomalous results when using commercially available biochemical tests and that some non-pathogenic species are still being misidentified using some biochemical kits. This further reinforces the recommendation that any dubious biochemical result should be confirmed with an immunological test.

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2005-09-01
2024-03-29
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References

  1. Boehm D. M., Bernhardt M., Kurzynski T. A., Pennell D. R., Schell R. F. 1990; Evaluation of two commercial procedures for rapid identification of Neisseria gonorrhoeae using a reference panel of antigenically diverse gonococci. J Clin Microbiol 28:2099–2100
    [Google Scholar]
  2. Boyce J. M., Mitchell E. B. 1985; Difficulties in differentiating Neisseria cinerea from Neisseria gonorrhoeae cultures by standard and experimental tests. J Clin Microbiol 22:731–734
    [Google Scholar]
  3. Dillon R. J., Carboallo M., Pauze M. 1988; Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, GonoBio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI test, and Syva MicroTrak Test. J Clin Microbiol 26:493–497
    [Google Scholar]
  4. HPA 2001; Prolyliminopeptidase-negative isolates of Neisseria gonorrhoeae . Commun Dis Rep CDR Wkly [serial online] 1149 Available at http://www.hpa.org.uk/cdr/archives/2001/cdr4901.pdf
    [Google Scholar]
  5. Kellogg S. D., Turner E. M. 1973; Rapid fermentation confirmation of Neisseria gonorrhoeae . Appl Microbiol 5:550–552
    [Google Scholar]
  6. Knapp S. J. 1988; Historical perspectives and identification of Neisseria and related species. Clin Microbiol Rev 1:415–431
    [Google Scholar]
  7. Knapp S. J., Totten P. A., Mulks M. H., Minshew B. H. 1984; Characterisation of Neisseria cinerea , a non-pathogenic species isolated on Martin-Lewis Medium selective for pathogenic Neisseria sp. J Clin Microbiol 19:63–67
    [Google Scholar]
  8. Laughon E. B., Ehret J. E., Tanino T. T., Van der Pol B., Handsfield H. H., Jones R. B., Judson F. N., Hook E. W. 1987; Fluorescent monoclonal antibody for confirmation of Neisseria gonorrhoeae cultures. J Clin Microbiol 25:2388–2390
    [Google Scholar]
  9. Robinson J. M., Oberhofer T. R. 1983; Identification of pathogenic Neisseria species with the RapID NH system. J Clin Microbiol 3:400–404
    [Google Scholar]
  10. Tam R. M., Buchanan T. M., Sandstrom E. G., Holmes K. K., Knapp J. S., Siadak A. W., Nowinski R. C. 1982; Serological classification of Neisseria gonorrhoeae with monoclonal antibodies. Infect Immun 36:1042–1053
    [Google Scholar]
  11. Welch D. W., Cartwright G. 1988; Fluorescent monoclonal antibody compared with carbohydrate utilization for rapid identification of Neisseria gonorrhoeae. J Clin Microbiol 26:293–296
    [Google Scholar]
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