1887

Journal of Medical Microbiology

Volume 55, Issue 3

Attachment of to human respiratory cell lines is inhibited by certain oligosaccharides Free

Abstract

Pneumonic plague is an aggressive disease that is clinically difficult to treat. Inhibition of attachment using oligosaccharide receptor mimics may provide an alternative to antibiotics. The virulent strain GB was demonstrated to attach to the murine monocyte cell line (J774A.1) and a range of human respiratory epithelial cell lines: nasal (RPMI-2650), bronchial (BEAS2-B) and alveolar (A549). Attachment was greatest to the A549 and BEAS2-B cell lines. Pre-treatment of the cell lines with tunicamycin reduced attachment by 55–65 %, indicating the importance of cell-surface carbohydrates in adhesion. The cell lines displayed differences in the oligosaccharides that inhibited attachment. -Nitrophenol was the best inhibitor for each cell line. Disaccharides such as GalNAc1-3Gal and GalNAc1-4Gal were also good inhibitors, particularly for the RPMI-2650 cell line. This demonstrates the potential of oligosaccharides as potential anti-adhesion therapeutics.

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Keyword(s): Pla, plasminogen activator and PsaA, pH6 antigen
© SGM
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2006-03-01
2019-10-18
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Attachment of Yersinia pestis to human respiratory cell lines is inhibited by certain oligosaccharides
J. Med. Microbiol. 55, 309 (2006); https://doi.org/10.1099/jmm.0.46102-0
/content/journal/jmm/10.1099/jmm.0.46102-0
/content/journal/jmm/10.1099/jmm.0.46102-0
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