1887

Abstract

The incidence of candidaemia among immunocompromised patients in Malaysia is increasing at an alarming rate. Isolation of clinical strains that are resistant to fluconazole has also risen markedly. We report here the repeated isolation of from the blood of a neonatal patient with Hirschsprung's disease. fluconazole susceptibility tests of the eight isolates obtained at different time points showed that seven of the isolates were resistant and one isolate was scored as susceptible dose-dependent. Random amplification of polymorphic DNA fingerprinting of the isolates using three primers and subsequent phylogenetic analysis revealed that these isolates were highly similar strains having minor genetic divergence, with a mean pairwise similarity coefficient of 0·893±0·041. The source of the infectious agent was thought to be the central venous catheter, as culture of its tip produced fluconazole-resistant . This study demonstrates the utility of applying molecular epidemiology techniques to complement traditional mycological culture and drug susceptibility tests for accurate and appropriate management of recurrent candidaemia and highlights the need for newer antifungals that can combat the emergence of fluconazole-resistant strains.

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2006-04-01
2019-10-18
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Supplements

vol. , part 4, pp. 423 - 428

Scoring of polymorphic bands (amplimers) in RAPD-PCR obtained from isolates using primers ERIC1 and ERIC2.

Scores of polymorphic bands in RAPD-PCR obtained from isolates using primer RP02.

Distance matrix for fingerprinting of eight isolates using RP02 and ERIC primers.

[PDF file of Supplementary Tables S1-S3](15 KB)



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