1887

Abstract

is a frequent cause of infectious diarrhoea and is increasingly recognized as a trigger for late-onset complications. The poor standardization of commonly used serological tests might explain the conflicting results regarding the frequency of antecedent infections in defined patient groups. In order to obtain reliable epidemiological data as to the role of in causing late-onset complications, a highly specific and sensitive diagnostic tool for the epidemiological investigation of -associated diseases was developed. It was shown that recombinant proteins encoded by the genes (P39) and (P18) are specifically recognized by antibodies in sera from patients with enteritis. An ELISA using recombinant P18 and P39 as antigens was 91.9 % sensitive and 99.0 % specific, with positive and negative predictive values of 97.1 % and 97.0 %, respectively, comparing favourably with the 27.0 % sensitivity of a routinely used serological assay.

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2005-08-01
2019-11-22
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