1887

Abstract

The present report describes the diagnostic strategy followed in a case of keratomycosis. Together with conventional methods, a molecular strategy that involved the direct sequencing of an amplified portion of the genome encompassing the internal transcribed spacer 1 and 2 regions and sequence analysis was used. The data highlight the diagnostic role of molecular techniques, in parallel with conventional methods, in the management of ocular infections of fungal aetiology.

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2005-09-01
2019-11-19
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References

  1. Bouza, E. & Munoz, P. ( 2004;). Invasive infections caused by Blastoschizomyces capitatus and Scedosporium spp. Clin Microbiol Infect 10 (Suppl 1), 76–85.
    [Google Scholar]
  2. Diaz-Valle, D., Benitez del Castillo, J. M., Amor, E., Toledano, N., Carretero, M. M. & Diaz-Valle, T. ( 2002;). Severe keratomycosis secondary to Scedosporium apiospermum. Cornea 21, 516–518.[CrossRef]
    [Google Scholar]
  3. Ferrer, C., Colom, F., Frases, S., Mulet, E., Abad, J. L. & Alio, J. L. ( 2001;). Detection and identification of fungal pathogens by PCR and by ITS2 and 5.8S ribosomal DNA typing in ocular infections. J Clin Microbiol 39, 2873–2879.[CrossRef]
    [Google Scholar]
  4. Fujita, S. I., Senda, Y., Nakaguchi, S. & Hashimoto, T. ( 2001;). Multiplex PCR using internal transcribed spacer 1 and 2 regions for rapid detection and identification of yeast strains. J Clin Microbiol 39, 3617–3622.[CrossRef]
    [Google Scholar]
  5. Guarro, J. & Gene, J. ( 2002;). Acrophialophora fusispora misidentified as Scedosporium prolificans. J Clin Microbiol 40, 3544. 3544.[CrossRef]
    [Google Scholar]
  6. Han, D. P., Wisniewski, S. R., Wilson, L. A., Barza, M., Vine, A. K., Doft, B. H. & Kelsey, S. F. ( 1996;). Spectrum and susceptibilities of microbiologic isolates in the Endophthalmitis Vitrectomy Study. Am J Ophthalmol 122, 1–17.[CrossRef]
    [Google Scholar]
  7. Iwen, P. C., Hinrichs, S. H. & Rupp, M. E. ( 2002;). Utilization of the internal transcribed spacer regions as molecular targets to detect and identify human fungal pathogens. Med Mycol 40, 87–109.[CrossRef]
    [Google Scholar]
  8. Klotz, S. A., Penn, C. C., Negvesky, G. J. & Butrus, S. I. ( 2000;). Fungal and parasitic infections of the eye. Clin Microbiol Rev 13, 662–685.[CrossRef]
    [Google Scholar]
  9. Kunimoto, D. Y., Das, T., Sharma, S., Jalali, S., Majji, A. B., Gopinathan, U., Athmanathan, S. & Rao, T. N. ( 1999a;). Microbiologic spectrum and susceptibility of isolates: part II.Posttraumatic endophthalmitis. Endophthalmitis Research Group. Am J Ophthalmol 128, 242–244.[CrossRef]
    [Google Scholar]
  10. Kunimoto, D. Y., Das, T., Sharma, S., Jalali, S., Majji, A. B., Gopinathan, U., Athmanathan, S. & Rao, T. N. ( 1999b;). Microbiologic spectrum and susceptibility of isolates: part I.Postoperative endophthalmitis. Endophthalmitis Research Group. Am J Ophthalmol 128, 240–242.[CrossRef]
    [Google Scholar]
  11. Nulens, E., Eggink, C., Rijs, A. J., Wesseling, P. & Verweij, P. E. ( 2003;). Keratitis caused by Scedosporium apiospermum successfully treated with a cornea transplant and voriconazole. J Clin Microbiol 41, 2261–2264.[CrossRef]
    [Google Scholar]
  12. Pryce, T. M., Palladino, S., Kay, I. D. & Coombs, G. W. ( 2003;). Rapid identification of fungi by sequencing the ITS1 and ITS2 regions using an automated capillary electrophoresis system. Med Mycol 41, 369–381.[CrossRef]
    [Google Scholar]
  13. Rumelt, S., Cohen, I., Lefler, E. & Rehany, U. ( 2001;). Corneal co-infection with Scedosporium apiospermum and Acanthamoeba after sewage-contaminated ocular injury. Cornea 20, 112–116.[CrossRef]
    [Google Scholar]
  14. Saracli, M. A., Erdem, U., Gonlum, A. & Yildiran, S. T. ( 2003;). Scedosporium apiospermum keratitis treated with itraconazole. Med Mycol 41, 111–114.
    [Google Scholar]
  15. Tanure, M. A., Cohen, E. J., Sudesh, S., Rapuano, C. J. & Laibson, P. R. ( 2000;). Spectrum of fungal keratitis at Wills Eye Hospital, Philadelphia, Pennsylvania. Cornea 19, 307–312.[CrossRef]
    [Google Scholar]
  16. Thomas, P. A. ( 2003;). Current perspectives on ophthalmic mycoses. Clin Microbiol Rev 16, 730–797.[CrossRef]
    [Google Scholar]
  17. Voigt, K., Cigelnik, E. & O'Donnell, K. ( 1999;). Phylogeny and PCR identification of clinically important Zygomycetes based on nuclear ribosomal-DNA sequence data. J Clin Microbiol 37, 3957–3964.
    [Google Scholar]
  18. White, T., Burns, T., Lee, S. & Taylor, J. ( 1990;). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols, pp. 315–322. Edited by M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. White. San Diego: Academic Press.
  19. Wu, Z., Ying, H., Yiu, S., Irvine, J. & Smith, R. ( 2002;). Fungal keratitis caused by Scedosporium apiospermum: report of two cases and review of treatment. Cornea 21, 519–523.[CrossRef]
    [Google Scholar]
  20. Yamada, Y., Makimura, K., Merhendi, H., Ueda, K., Nishiyama, Y., Yamaguchi, H. & Osumi, M. ( 2002;). Comparison of different methods for extraction of mitochondrial DNA from human pathogenic yeasts. Jpn J Infect Dis 55, 122–125.
    [Google Scholar]
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