1887

Abstract

The morbidity and mortality rates in patients with cystic fibrosis (CF) are significantly affected by infections with complex. In a Czech CF Centre, the prevalence of the infection reached up to 30 %, with the majority of patients found to be infected with (formerly genomovar III of the complex). Since is associated with patient-to-patient transmission and epidemic outbreaks among CF patients, this study sought to examine the epidemiological relatedness between the Czech isolates belonging to the genomovar-homogeneous group. Eighty-three clinical isolates recovered from 67 CF patients were analysed using a random amplified polymorphic DNA (RAPD) assay and macrorestriction typing (I and I) followed by PFGE. A single predominant banding pattern shared by multiple isolates was detected, although I-generated PFGE results yielded a higher rate of inter-pattern variability in comparison to the more uniform RAPD and I-generated PFGE results for this clone. Both typing systems also showed that only three out of 67 patients harboured strains distinct from the major strain type. The dominant clone was characterized by PCR positivity for the epidemic strain marker, PCR negativity for the cable pilin subunit gene and close genetic relatedness to the epidemic strain of RAPD 01 type previously identified in Canada.

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2005-07-01
2019-11-17
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