%0 Journal Article %A Winstanley, Craig %A Kaye, Stephen B %A Neal, Timothy J %A Chilton, Helen J %A Miksch, Silvia %A Hart, C Anthony %A and the Microbiology Ophthalmic Group %T Genotypic and phenotypic characteristics of Pseudomonas aeruginosa isolates associated with ulcerative keratitis %D 2005 %J Journal of Medical Microbiology, %V 54 %N 6 %P 519-526 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.46005-0 %I Microbiology Society, %X A collection of 63 isolates of Pseudomonas aeruginosa associated with ulcerative keratitis, collected from six centres in England, were typed using serotyping and random amplified polymorphic DNA-PCR, and screened for several variable virulence-related genotypes and phenotypes. Sixty-one percent of the isolates were of either serotype O1 or serotype O11, but there was no evidence for a common clone. The majority of isolates (59 %) were PCR-positive for exoU rather than for exoS (38 %), and carried a-type fliC genes (76 %) rather than b-type (24 %). Isolates were PCR-positive for pyoverdine-receptor types at a prevalence of 38 % for type I, 46 % for type II and 8 % for type III. All but one of the isolates exhibited twitching activity. There was a correlation between the presence of exoS and twitching activity (P = 0.04), suggesting that a combination of exoS genotype and good twitching activity may have a role to play in ExoU-independent corneal virulence. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.46005-0