1887

Abstract

A collection of 63 isolates of associated with ulcerative keratitis, collected from six centres in England, were typed using serotyping and random amplified polymorphic DNA-PCR, and screened for several variable virulence-related genotypes and phenotypes. Sixty-one percent of the isolates were of either serotype O1 or serotype O11, but there was no evidence for a common clone. The majority of isolates (59 %) were PCR-positive for rather than for (38 %), and carried a-type genes (76 %) rather than b-type (24 %). Isolates were PCR-positive for pyoverdine-receptor types at a prevalence of 38 % for type I, 46 % for type II and 8 % for type III. All but one of the isolates exhibited twitching activity. There was a correlation between the presence of and twitching activity ( = 0.04), suggesting that a combination of genotype and good twitching activity may have a role to play in ExoU-independent corneal virulence.

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2005-06-01
2021-07-29
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