RT Journal Article SR Electronic(1) A1 Imamura, Yoshifumi A1 Yanagihara, Katsunori A1 Tomono, Kazunori A1 Ohno, Hideaki A1 Higashiyama, Yasuhito A1 Miyazaki, Yoshitsugu A1 Hirakata, Yoichi A1 Mizuta, Yohei A1 Kadota, Jun-ichi A1 Tsukamoto, Kazuhiro A1 Kohno, ShigeruYR 2005 T1 Role of Pseudomonas aeruginosa quorum-sensing systems in a mouse model of chronic respiratory infection JF Journal of Medical Microbiology, VO 54 IS 6 SP 515 OP 518 DO https://doi.org/10.1099/jmm.0.46004-0 PB Microbiology Society, SN 1473-5644, AB The role of quorum-sensing systems in a mouse model of chronic Pseudomonas aeruginosa infection was studied. A chronic P. aeruginosa respiratory infection model was established by placement of a tube pre-coated with strain PAO1 (wild-type) or a quorum-sensing mutant, namely PAO-JP1 (ΔlasI), PDO100 (ΔrhlI) or PAO-JP2 (ΔlasI/ΔrhlI), in the bronchus. At day 14 after infection, the numbers of viable bacteria in the quorum-sensing-mutant groups were lower than in the wild-type group. Histopathological examination showed milder inflammatory changes in the lungs infected with the mutant groups compared with the wild-type group. In the bronchoalveolar lavage fluid from the quorum-sensing-system-mutant groups the proportion of neutrophils was lower than in wild-type group. These findings indicate that the quorum-sensing system plays an important role in chronic P. aeruginosa respiratory infection., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.46004-0