%0 Journal Article %A Imamura, Yoshifumi %A Yanagihara, Katsunori %A Tomono, Kazunori %A Ohno, Hideaki %A Higashiyama, Yasuhito %A Miyazaki, Yoshitsugu %A Hirakata, Yoichi %A Mizuta, Yohei %A Kadota, Jun-ichi %A Tsukamoto, Kazuhiro %A Kohno, Shigeru %T Role of Pseudomonas aeruginosa quorum-sensing systems in a mouse model of chronic respiratory infection %D 2005 %J Journal of Medical Microbiology, %V 54 %N 6 %P 515-518 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.46004-0 %I Microbiology Society, %X The role of quorum-sensing systems in a mouse model of chronic Pseudomonas aeruginosa infection was studied. A chronic P. aeruginosa respiratory infection model was established by placement of a tube pre-coated with strain PAO1 (wild-type) or a quorum-sensing mutant, namely PAO-JP1 (ΔlasI), PDO100 (ΔrhlI) or PAO-JP2 (ΔlasI/ΔrhlI), in the bronchus. At day 14 after infection, the numbers of viable bacteria in the quorum-sensing-mutant groups were lower than in the wild-type group. Histopathological examination showed milder inflammatory changes in the lungs infected with the mutant groups compared with the wild-type group. In the bronchoalveolar lavage fluid from the quorum-sensing-system-mutant groups the proportion of neutrophils was lower than in wild-type group. These findings indicate that the quorum-sensing system plays an important role in chronic P. aeruginosa respiratory infection. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.46004-0