1887

Abstract

In the present study, it was shown that repetitive-DNA-element PCR fingerprinting using the (GTG) primer [(GTG)-PCR] is a rapid and reliable tool to genotypically differentiate members of the recently described pan-European multi-resistant (MAB) clone III from the known MAB clones I and II. The identification of four new representatives of the MAB clone III dating from 1991 to 1993 by (GTG)-PCR indicates that this clone has persisted in European hospitals since the beginning of the 1990s. Tetracycline (TET) resistance was found to be common among clone III strains, including one strain that also displayed resistance to minocycline. The TET-resistance phenotype in this MAB clone appeared to be strongly associated with the presence of the efflux-type gene (A), but the fact that some members lack this gene or have acquired an additional gene [i.e. (M)] suggests that the gene carriage in the pan-European clone III population may have diversified in time and space. In contrast, all clone III strains shared the previously described aminoglycoside resistotype encoded by the aminoglycoside-modifying genes and the class 1 integron-associated , which may point to the fact that these genes probably are more stably inherited in MAB clone III compared to genes.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.45986-0
2005-09-01
2019-09-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/54/9/JM540907.html?itemId=/content/journal/jmm/10.1099/jmm.0.45986-0&mimeType=html&fmt=ahah

References

  1. Bergogne-Bérézin, E. & Towner, K. J. ( 1996;). Acinetobacter spp.as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev 9, 148–165.
    [Google Scholar]
  2. Bou, G., Cervero, G., Dominguez, M. A., Quereda, C. & Martinez-Beltran, J. ( 2000;). PCR-based DNA fingerprinting (REP-PCR, AP-PCR) and pulsed-field gel electrophoresis characterization of a nosocomial outbreak caused by imipenem- and meropenem-resistant Acinetobacter baumannii. Clin Microbiol Infect 6, 635–643.[CrossRef]
    [Google Scholar]
  3. Brisse, S., Milatovic, D., Fluit, A. C., Kusters, K., Toelstra, A., Verhoef, J. & Schmitz, F.-J. ( 2000;). Molecular surveillance of European quinolone-resistant clinical isolates of Pseudomonas aeruginosa and Acinetobacter spp.using automated ribotyping. J Clin Microbiol 38, 3636–3645.
    [Google Scholar]
  4. Canton, R., Coque, T. M. & Baquero, F. ( 2003;). Multi-resistant Gram-negative bacilli: from epidemics to endemics. Curr Opin Infect Dis 16, 315–325.[CrossRef]
    [Google Scholar]
  5. Dijkshoorn, L., Aucken, H., Gerner-Smidt, P., Janssen, P., Kaufmann, M. E., Garaizar, J., Ursing, J. & Pitt, T. L. ( 1996;). Comparison of outbreak and nonoutbreak Acinetobacter baumannii strains by genotypic and phenotypic methods. J Clin Microbiol 34, 1519–1525.
    [Google Scholar]
  6. Gevers, D., Huys, G. & Swings, J. ( 2001;). Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205, 31–36.[CrossRef]
    [Google Scholar]
  7. Huys, G., Cnockaert, M., Vaneechoutte, M., Woodford, N., Nemec, A., Dijkshoorn, L. & Swings, J. ( 2005;). Distribution of tetracycline resistance genes in genotypically related and unrelated multi-resistant Acinetobacter baumannii strains from different European hospitals. Res Microbiol 156, 348–355.[CrossRef]
    [Google Scholar]
  8. Kuo, L.-C., Teng, L.-J., Yu, C.-J., Ho, S.-W. & Hsueh, P.-R. ( 2004;). Dissemination of a clone of unusual phenotype of pandrug-resistant Acinetobacter baumannii at a university hospital in Taiwan. J Clin Microbiol 42, 1759–1763.[CrossRef]
    [Google Scholar]
  9. Lopez-Otsoa, F., Gallego, L., Towner, K. J., Tysall, L., Woodford, N. & Livermore, D. M. ( 2002;). Endemic carbapenem resistance associated with OXA-40 carbapenemase among Acinetobacter baumannii isolates from a hospital in northern Spain. J Clin Microbiol 40, 4741–4743.[CrossRef]
    [Google Scholar]
  10. Magnet, S., Courvalin, P. & Lambert, T. ( 2001;). Resistance-nodulation-cell division-type efflux pump involved in aminoglycoside resistance in Acinetobacter baumannii strain BM4454. Antimicrob Agents Chemother 45, 3375–3380.[CrossRef]
    [Google Scholar]
  11. NCCLS ( 2000;). Performance Standards for Antimicrobial Disc Susceptibility Testing. Approved Standard M2-A7. Wayne, PA: National Committee for Clinical Laboratory Standards.
  12. NCCLS ( 2003;). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically. Approved Standard M7-A6. Wayne, PA: National Committee for Clinical Laboratory Standards.
  13. Nemec, A., De Baere, T., Tjernberg, I., Vaneechoutte, M., van der Reijden, T. J. K. & Dijkshoorn, L. ( 2001;). Acinetobacter ursingii sp.nov. and Acinetobacter schindleri sp. nov., isolated from human clinical specimens. Int J Syst Evol Microbiol 51, 1891–1899.[CrossRef]
    [Google Scholar]
  14. Nemec, A., Dijkshoorn, L. & van der Reijden, T. J. K. ( 2004a;). Long-term predominance of two pan-European clones among multi-resistant Acinetobacter baumannii strains in the Czech Republic. J Med Microbiol 53, 147–153.[CrossRef]
    [Google Scholar]
  15. Nemec, A., Dolzani, L., Brisse, S., van den Broek, P. & Dijkshoorn, L. ( 2004b;). Diversity of aminoglycoside-resistance genes and their association with class 1 integrons among strains of pan-European Acinetobacter baumannii clones. J Med Microbiol 53, 1233–1240.[CrossRef]
    [Google Scholar]
  16. Quale, J., Bratu, S., Landman, D. & Heddurshetti, R. ( 2003;). Molecular epidemiology and mechanisms of carbapenem resistance in Acinetobacter baumannii endemic in New York city. Clin Infect Dis 37, 214–220.[CrossRef]
    [Google Scholar]
  17. van Dessel, H., Dijkshoorn, L., Van der Reijden, T., Bakker, N., Paauw, A., van den Broek, P., Verhoef, J. & Brisse, S. ( 2004;). Identification of a new geographically widespread multiresistant Acinetobacter baumannii clone from European hospitals. Res Microbiol 155, 105–112.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.45986-0
Loading
/content/journal/jmm/10.1099/jmm.0.45986-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error