Use of chromogenic medium in the isolation of yeasts from clinical specimens Free

Abstract

Over a 1 year period 3296 specimens submitted for fungal culture were plated onto routine mycological media (RM) and CHROMagar Candida (CaC) to evaluate the capability of CaC to improve on RM. With RM, cultures producing single yeast isolates were identified from 802 specimens. CaC produced similar results, with 76 % agreement. Of 761 specimens that yielded a single species by RM, 615 (81 %) produced one or more yeast isolates using CaC. Of concern, 132 negative CaC cultures corresponded to specimens that yielded alone on RM. When yeasts were recovered, CaC correctly identified 98 % of , 93 % of , 96 % of and 100 % of based on typical colours. CaC did potentially improve on RM by detecting yeasts in 91 specimens that yielded none by routine methods. CaC was noted to recover more yeast isolates than RM when mixed cultures were detected. Overall, the role of CaC in improving RM appears limited.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.45942-0
2005-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/54/10/JM541013.html?itemId=/content/journal/jmm/10.1099/jmm.0.45942-0&mimeType=html&fmt=ahah

References

  1. Ainscough S., Kibbler C. C. 1998; An evaluation of the cost-effectiveness of using CHROMagar for yeast identification in a routine microbiology laboratory. J Med Microbiol 47:623–628 [CrossRef]
    [Google Scholar]
  2. Baumgartner C., Freydiere A. M., Gille Y. 1996; Direct identification and recognition of yeast species from clinical material by using Albicans ID and CHROMagar Candida plates. J Clin Microbiol 34:454–456
    [Google Scholar]
  3. Beck-Sague C., Jarvis W. R. 1993; National Nosocomial Infections Surveillance System: secular trends in the epidemiology of nosocomial fungal infections in the United States, 1980–1990. J Infect Dis 167:1247–1251 [CrossRef]
    [Google Scholar]
  4. Beighton D., Ludford R., Clark D. T. & 9 other authors; 1995; Use of CHROMagar Candida medium for isolation of yeasts from dental samples. J Clin Microbiol 33:3025–3027
    [Google Scholar]
  5. Bernal S., Mazuelos E. M., Garcia M., Aller A. I., Martinez M. A., Gutierrez M. J. 1996; Evaluation of CHROMagar Candida medium for the isolation and presumptive identification of species of Candida of clinical importance. Diagn Microbiol Infect Dis 24:201–204 [CrossRef]
    [Google Scholar]
  6. Bouchara J. P., Declerck P., Cimon B., Planchenault C., de Gentile L., Chabasse D. 1996; Routine use of CHROMagar Candida medium for presumptive identification of Candida yeast species and detection of mixed fungal populations. Clin Microbiol Infect 2:202–208 [CrossRef]
    [Google Scholar]
  7. Cooke V. M., Miles R. J., Price R. G., Midgley G., Khamri W., Richardson A. C. 2002; New chromogenic agar medium for the identification of Candida spp. Appl Environ Microbiol 68:3622–3627 [CrossRef]
    [Google Scholar]
  8. Edmond M. B., Wallace S. E., McClish D. K., Pfaller M. A., Jones R. N., Wenzel R. P. 1999; Nosocomial bloodstream infections in United States hospitals: a three-year analysis. Clin Infect Dis 29:239–244 [CrossRef]
    [Google Scholar]
  9. Freydiere A. M., Buchaille L., Gille Y. 1997; Comparison of three commercial media for direct identification and discrimination of Candida species in clinical specimens. Eur J Clin Microbiol Infect Dis 16:464–467 [CrossRef]
    [Google Scholar]
  10. Hospenthal D. R., Murray C. K., Beckius M. L., Green J. A., Dooley D. P. 2002; Persistence of pigment production by yeast isolates grown on CHROMagar Candida media. J Clin Microbiol 40:4768–4770 [CrossRef]
    [Google Scholar]
  11. Hospenthal D. R., Murray C. K., Rinaldi M. G. 2004; The role of antifungal susceptibility testing in the therapy of candidiasis. Diagn Microbiol Infect Dis 48:153–160 [CrossRef]
    [Google Scholar]
  12. Houang E. T., Chu K. C., Koehler A. P., Cheng A. F. 1997; Use of CHROMagar Candida for genital specimens in the diagnostic laboratory. J Clin Pathol 50:563–565 [CrossRef]
    [Google Scholar]
  13. Kirkpatrick W. R., Revankar S. G., McAtee R. K. & 7 other authors; 1998; Detection of Candida dubliniensis in oropharyngeal samples from human immunodeficiency virus-infected patients in North America by primary CHROMagar Candida screening and susceptibility testing of isolates. J Clin Microbiol 36:3007–3012
    [Google Scholar]
  14. Lopez-Ribot J. L., McAtee R. K., Perea S., Kirkpatrick W. R., Rinaldi M. G., Patterson T. F. 1999; Multiple resistant phenotypes of Candida albicans coexist during episodes of oropharyngeal candidiasis in human immunodeficiency virus-infected patients. Antimicrob Agents Chemother 43:1621–1630
    [Google Scholar]
  15. Momani O. M. 2000; Cost-effectiveness and efficacy of CHROMagar Candida medium in clinical specimens. East Mediterr Health J 6:968–978
    [Google Scholar]
  16. Odds F. C., Bernaerts R. 1994; CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species. J Clin Microbiol 32:1923–1929
    [Google Scholar]
  17. Odds F. C., Davidson A. 2000; ‘Room temperature’ use of CHROMagar Candida. Diagn Microbiol Infect Dis 38:147–150 [CrossRef]
    [Google Scholar]
  18. Pfaller M. A., Houston A., Coffmann S. 1996; Application of CHROMagar Candida for rapid screening of clinical specimens for Candida albicans , Candida tropicalis , Candida krusei ,and Candida ( Torulopsis ) glabrata .. J Clin Microbiol 34:58–61
    [Google Scholar]
  19. Rangel-Frausto M. S., Wiblin T., Blumberg H. M. & 8 other authors; 1999; National Epidemiology of Mycoses Survey (NEMIS): variations in rates of bloodstream infections due to Candida species in seven surgical intensive care units and six neonatal intensive care units. Clin Infect Dis 29:253–258 [CrossRef]
    [Google Scholar]
  20. Redding S. W., Zellars R. C., Kirkpatrick W. R. & 7 other authors; 1999; Epidemiology of oropharyngeal Candida colonization and infection in patients receiving radiation for head and neck cancer. J Clin Microbiol 37:3896–3900
    [Google Scholar]
  21. Willinger B., Manafi M. 1999; Evaluation of CHROMagar Candida for rapid screening of clinical specimens for Candida species. Mycoses 42:61–65 [CrossRef]
    [Google Scholar]
  22. Willinger B., Hillowoth C., Selitsch B., Manafi M. 2001; Performance of Candida ID, a new chromogenic medium for presumptive identification of Candida species, in comparison to CHROMagar Candida. J Clin Microbiol 39:3793–3795 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.45942-0
Loading
/content/journal/jmm/10.1099/jmm.0.45942-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed