1887

Abstract

Th1 immune responses in which gamma interferon (IFN-γ) production predominates are associated with protective immunity against intracellular bacteria. Following infection, interleukin-18 (IL-18) may contribute, in association with IL-12, to optimal IFN-γ production. In this study, the secretion of IL-18 following intracellular infection with virulent 2308 in CD-1 cultured peritoneal macrophages and splenocyte cultures was investigated. The production of IL-18 was reduced in both CD-1 mouse peritoneal macrophages infected with 2308 and splenocyte cultures obtained from 2308-infected mice at 3, 6 and 10 days post-infection (p.i.). In contrast, splenocyte cultures obtained from 2308-infected mice at 3 days p.i. secreted significant amounts of IFN-γ. Stimulation of these cells with recombinant IL-18 (rIL-18) and/or rIL-12 did not significantly increase IFN-γ secretion at the splenocyte level. These data suggest that once the infection has been established, 2308 selectively limits IL-18 secretion without affecting endogenous IFN-γ production.

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2005-06-01
2019-11-20
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References

  1. Araya, L. N., Elzer, P. H., Rowe, G. E., Enright, F. M. & Winter, A. J. ( 1989;). Temporal development of protective cell-mediated and humoral immunity in BALB/c mice infected with Brucella abortus. J Immunol 143, 3330–3337.
    [Google Scholar]
  2. Bohn, E., Sing, A., Zumbihl, R., Brielfeldt, C., Okamura, H., Kurimoto, M., Heesemann, J. & Autenrieth, I. B. ( 1998;). IL-18 (IFN-γ-inducing factor) regulates early cytokine production in, and promotes resolution of, bacterial infection in mice. J Immunol 160, 299–307.
    [Google Scholar]
  3. Elhofy, A. & Bost, K. ( 1999;). Limited interleukin-18 response in Salmonella-infected murine macrophages and in Salmonella-infected mice. Infect Immun 67, 5021–5026.
    [Google Scholar]
  4. Fernández-Lago, L., Rodríguez-Tarazona, E. & Vizcaíno, N. ( 1999;). Differential secretion of interleukin-12 (IL-12) subunits and heterodimeric IL-12p70 protein by CD-1 mice and murine macrophages in response to intracellular infection by Brucella abortus. J Med Microbiol 48, 1065–1073.[CrossRef]
    [Google Scholar]
  5. Gerdes, N., Sukhova, G. K., Libby, P., Reynolds, R. S., Young, J. L. & Schonbeck, U. ( 2002;). Expression of interleukin (IL)-18 and functional IL-18 receptor on human vascular endothelial cells, smooth muscle cells, and macrophages: implications for atherogenesis. J Exp Med 195, 245–257.[CrossRef]
    [Google Scholar]
  6. Ghayur, T., Banerjee, S., Hugunin, M. & 11 other authors ( 1997;). Caspase-1 processes IFN-gamma-inducing factor and regulates LPS-induced IFN-gamma production. Nature 386, 619–626.[CrossRef]
    [Google Scholar]
  7. Jiang, X. & Baldwin, C. L. ( 1993;). Effects of cytokines on intracellular growth of Brucella abortus. Infect Immun 61, 124–134.
    [Google Scholar]
  8. Joubier-Maurin, V., Boigegrain, R. A., Cloeckaert, A. & 8 other authors ( 2001;). Major outer membrane protein Omp25 of Brucella suis is involved in inhibition of tumor necrosis factor alpha production during infection of human macrophages. Infect Immun 69, 4823–4830.[CrossRef]
    [Google Scholar]
  9. Lauw, F. N., Branger, J., Florquin, S., Speelman, P., van Deventer, S. J. H., Akira, S. & van der Poll, T. ( 2002;). IL-18 improves the early antimicrobial host response to pneumococcal pneumonia. J Immunol 168, 372–378.[CrossRef]
    [Google Scholar]
  10. Mastroeni, P. S., Clare, S., Khan, S., Harrison, J. A., Hormaeche, C. E., Okamura, H., Kurimoto, M. & Dougan, G. ( 1999;). Interleukin 18 contributes to host resistance and gamma interferon production in mice infected with virulent Salmonella typhimurium. Infect Immun 67, 478–483.
    [Google Scholar]
  11. Neighbors, M., Xu, X., Barrat, F. J. & 7 other authors ( 2001;). A critical role for interleukin 18 in primary and memory effector responses to Listeria monocytogenes that extend beyond its effects on interferon gamma production. J Exp Med 194, 343–354.[CrossRef]
    [Google Scholar]
  12. Netea, M. G., Kullberg, B. J., Verschueren, I. & van der Meer, J. W. ( 2000;). Interleukin-18 induces production of proinflammatory cytokines in mice: no intermediate role for the cytokines of the tumor necrosis factor family and interleukin-1β. J Immunol 30, 3057–3060.
    [Google Scholar]
  13. Ohkusu, K., Yoshimoto, T., Takeda, K., Ogura, T., Kashiwamura, S.-I., Iwakura, Y., Akira, S., Okamura, H. & Nakanishi, K. ( 2000;). Potentiality of interleukin-18 as a useful reagent for treatment and prevention of Leishmania major infection. Infect Immun 68, 2449–2456.[CrossRef]
    [Google Scholar]
  14. Pasquali, P., Adone, R., Gasbarre, L. C., Pistola, C. & Ciuchini, F. ( 2001;). Mouse cytokine profiles associated with Brucella abortus RB51 vaccination or B.abortus 2308 infection. Infect Immun 69, 6541–6544.[CrossRef]
    [Google Scholar]
  15. Pasquali, P., Adone, R., Gasbarre, L. C., Pistoia, C. & Ciuchini, F. ( 2002;). Effect of exogenous interleukin-18 (IL-18) and IL-12 in the course of Brucella abortus 2308 infection in mice. Clin Diag Lab Immunol 9, 491–492.
    [Google Scholar]
  16. Pizarro-Cerdá, J., Moreno, E., Sanguedolce, V., Mege, J. L. & Gorvel, J. P. ( 1998;). Virulent Brucella abortus prevents lysosome fusion and is distributed within autophagosome-like compartments. Infect Immun 66, 2387–2392.
    [Google Scholar]
  17. Schultz, M. J., Knapp, S., Florquin, S., Pater, J., Takeda, K., Akira, S. & van der Poll, T. ( 2003;). Interleukin-18 impairs the pulmonary host response to Pseudomonas aeruginosa. Infect Immun 71, 1630–1634.[CrossRef]
    [Google Scholar]
  18. Stuyt, R. J., Netea, M. G., van Krieken, J. H., van der Meer, J. W. & Kullberg, B. J. ( 2004;). Recombinant interleukin-18 protects against disseminated Candida albicans infection in mice. J Infect Dis 189, 1524–1527.[CrossRef]
    [Google Scholar]
  19. Sugawara, I., Yamada, H., Kaneko, H., Mizuno, S., Takeda, K. & Akira, S. ( 1999;). Role of interleukin-18 (IL-18) in mycobacterial infection in IL-18-gene-disrupted mice. Infect Immun 67, 2585–2589.
    [Google Scholar]
  20. Takeda, K., Tsutsui, H., Yoshimoto, T., Adachi, O., Yoshida, N., Kishimoto, T., Okamura, H., Nakanishi, K. & Akira, S. ( 1998;). Defective NK cell activity and Th1 response in IL-18-deficient mice. Immunity 8, 383–390.[CrossRef]
    [Google Scholar]
  21. Vizcaíno, N., Chordi, A. & Fernández-Lago, L. ( 1991;). Characterization of smooth Brucella lipopolysaccharides and polysaccharides by monoclonal antibodies. Res Microbiol 142, 971–978.[CrossRef]
    [Google Scholar]
  22. Yoshimoto, T., Nagai, N., Ohkusu, K., Ueda, H., Okamura, H. & Nakanishi, K. ( 1998;). IL-12 up-regulates IL-18 receptor expression on T cells, Th1 cells, and B cells: synergism with IL-18 for IFN-γ production. J Immunol 161, 3400–3407.
    [Google Scholar]
  23. Zhan, Y. & Cheers, C. ( 1995;). Endogenous interleukin-12 is involved in resistance to Brucella abortus infection. Infect Immun 63, 1387–1390.
    [Google Scholar]
  24. Zhan, Y., Yang, J. & Cheers, C. ( 1993;). Cytokine response of T-cell subsets from Brucella abortus-infected mice to soluble Brucella proteins. Infect Immun 61, 2841–2847.
    [Google Scholar]
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