1887

Abstract

In this study penicillin-binding proteins (PBPs) of and the resistance mechanisms of this micro-organism to 11 β-lactam antibiotics were analysed. The study focused on the role of PBP2Bfr and metallo-β-lactamase in the mechanism of resistance to imipenem. The mechanism of β-lactam resistance in was strain dependent. The gene encoding the orthologue of PBP3 gene (, which encodes the protein PBP2Bfr) was sequenced in five of the eight strains studied, along with the () gene in strain 119, and their implications for resistance were examined. Differences were found in the amino-acid sequence of PBP2Bfr in strains AK-2 and 119, and the production of β-lactamases indicated that these differences may be involved in the mechanism of resistance to imipenem. binding competition assays with membrane extracts using imipenem indicated that the PBP that bound imipenem with the highest affinity was PBP2Bfr, and that increased affinity in strain 7160 may be responsible for the moderate susceptibility of this strain to imipenem. In the same way, the importance of the chromosomal class A β-lactamase CepA in the resistance mechanism of the strains NCTC 9344, 7160, 2013E, AK-4, 0423 and R-212 was studied. In these strains this is the principal resistance mechanism to antimicrobial agents studied other than imipenem.

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2005-11-01
2019-09-20
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vol. , part 11, pp. 1055 - 1064

Amino acid sequence alignment data for PBP2Bfr from several strains. [PDF](28 KB)



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