1887

Abstract

causes the development of gastritis, gastric ulcers and adenocarcinomas in humans. The establishment of infection is influenced by adherence to the gastric epithelium, and several bacterial adhesins and host cell receptors have been identified. recognize the Lewis receptor through the BabA adhesin but also readily adhere to epithelia in the absence of the Lewis epitope, demonstrating the relevance of additional adhesive interactions. This study presents a novel method of identifying bacterial adhesins. Nickel beads were coated with -derived, recombinantly expressed ORF proteins, and epithelial cells from the human stomach, intestine or urinary tract were allowed to adhere to those beads. The binding of epithelial cells to the protein-coated nickel beads was confirmed by electron microscopy or flow cytometry using antibodies directed towards the His-tags. Among the five ORFs tested, two new adhesive proteins (HP1188 and HP1430) were identified. Both were expressed on the surface of virulent , with the HP1188 protein being most abundant. The purified HP1188 and HP1430 proteins bound more strongly to gastric than to other epithelial cell lines, suggesting that they may be involved in the colonization of the human gastric mucosa. In conclusion, this method facilitates the identification of ORFs of microbial origin involved in cellular interactions such as adherence.

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2005-05-01
2019-11-18
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References

  1. Agace, W., Hedges, S., Andersson, U., Andersson, J., Ceska, M. & Svanborg, C. ( 1993;). Selective cytokine production by epithelial cells following exposure to Escherichia coli. Infect Immun 61, 602–609.
    [Google Scholar]
  2. Agace, W. W., Patarroyo, M., Svensson, M., Carlemalm, E. & Svanborg, C. ( 1995;). Escherichia coli induces transuroepithelial neutrophil migration by an intercellular adhesion molecule-1-dependent mechanism. Infect Immun 63, 4054–4062.
    [Google Scholar]
  3. Akopyanz, N., Bukanov, N. O., Westblom, T. U. & Berg, D. E. ( 1992;). PCR-based RFLP analysis of DNA sequence diversity in the gastric pathogen Helicobacter pylori. Nucleic Acids Res 20, 6221–6225.[CrossRef]
    [Google Scholar]
  4. Ascencio, F., Fransson, L. A. & Wadstrom, T. ( 1993;). Affinity of the gastric pathogen Helicobacter pylori for the N-sulphated glycosaminoglycan heparan sulphate. J Med Microbiol 38, 240–244.[CrossRef]
    [Google Scholar]
  5. Blaser, M. J. ( 1990;). Helicobacter pylori and the pathogenesis of gastroduodenal inflammation. J Infect Dis 161, 626–633.[CrossRef]
    [Google Scholar]
  6. Blaser, M. J. ( 1992;). Hypotheses on the pathogenesis and natural history of Helicobacter pylori-induced inflammation. Gastroenterology 102, 720–727.
    [Google Scholar]
  7. Boren, T., Falk, P., Roth, K. A., Larson, G. & Normark, S. ( 1993;). Attachment of Helicobacter pylori to human gastric epithelium mediated by blood group antigens. Science 262, 1892–1895.[CrossRef]
    [Google Scholar]
  8. Boren, T., Normark, S. & Falk, P. ( 1994;). Helicobacter pylori: molecular basis for host recognition and bacterial adherence. Trends Microbiol 2, 221–228.[CrossRef]
    [Google Scholar]
  9. Clyne, M. & Drumm, B. ( 1996;). Cell envelope characteristics of Helicobacter pylori: their role in adherence to mucosal surfaces and virulence. FEMS Immunol Med Microbiol 16, 141–155.[CrossRef]
    [Google Scholar]
  10. Clyne, M. & Drumm, B. ( 1997;). Absence of effect of Lewis A and Lewis B expression on adherence of Helicobacter pylori to human gastric cells. Gastroenterology 113, 72–80.[CrossRef]
    [Google Scholar]
  11. Edwards, N. J., Monteiro, M. A., Faller, G., Walsh, E. J., Moran, A. P., Roberts, I. S. & High, N. J. ( 2000;). Lewis X structures in the O antigen side-chain promote adhesion of Helicobacter pylori to the gastric epithelium. Mol Microbiol 35, 1530–1539.
    [Google Scholar]
  12. Falk, P. G., Bry, L., Holgersson, J. & Gordon, J. I. ( 1995;). Expression of a human alpha-1,3/4-fucosyltransferase in the pit cell lineage of FVB/N mouse stomach results in production of Leb-containing glycoconjugates: a potential transgenic mouse model for studying Helicobacter pylori infection. Proc Natl Acad Sci U S A 92, 1515–1519.[CrossRef]
    [Google Scholar]
  13. Frendeus, B., Wachtler, C., Hedlund, M., Fischer, H., Samuelsson, P., Svensson, M. & Svanborg, C. ( 2001;). Escherichia coli P fimbriae utilize the Toll-like receptor 4 pathway for cell activation. Mol Microbiol 40, 37–51.[CrossRef]
    [Google Scholar]
  14. Godaly, G., Proudfoot, A. E., Offord, R. E., Svanborg, C. & Agace, W. W. ( 1997;). Role of epithelial interleukin-8 (IL-8) and neutrophil IL-8 receptor A in Escherichia coli-induced transuroepithelial neutrophil migration. Infect Immun 65, 3451–3456.
    [Google Scholar]
  15. Graham, D. Y., Klein, P. D., Opekun, A. R. & Boutton, T. W. ( 1988;). Effect of age on the frequency of active Campylobacter pylori infection diagnosed by the [13C]urea breath test in normal subjects and patients with peptic ulcer disease. J Infect Dis 157, 777–780.[CrossRef]
    [Google Scholar]
  16. Hedges, S., Svensson, M. & Svanborg, C. ( 1992;). Interleukin-6 response of epithelial cell lines to bacterial stimulation in vitro. Infect Immun 60, 1295–1301.
    [Google Scholar]
  17. Hedlund, M., Svensson, M., Nilsson, A., Duan, R. D. & Svanborg, C. ( 1996;). Role of the ceramide-signaling pathway in cytokine responses to P-fimbriated Escherichia coli. J Exp Med 183, 1037–1044.[CrossRef]
    [Google Scholar]
  18. Hedlund, M., Duan, R. D., Nilsson, A. & Svanborg, C. ( 1998;). Sphingomyelin, glycosphingolipids and ceramide signalling in cells exposed to P-fimbriated Escherichia coli. Mol Microbiol 29, 1297–1306.[CrossRef]
    [Google Scholar]
  19. Hedlund, M., Frendeus, B., Wachtler, C., Hang, L., Fischer, H. & Svanborg, C. ( 2001;). Type 1 fimbriae deliver an LPS- and TLR4-dependent activation signal to CD14-negative cells. Mol Microbiol 39, 542–552.[CrossRef]
    [Google Scholar]
  20. Heneghan, M. A., McCarthy, C. F. & Moran, A. P. ( 2000;). Relationship of blood group determinants on Helicobacter pylori lipopolysaccharide with host lewis phenotype and inflammatory response. Infect Immun 68, 937–941.[CrossRef]
    [Google Scholar]
  21. Ilver, D., Arnqvist, A., Ogren, J. & 7 other authors ( 1998;). Helicobacter pylori adhesin binding fucosylated histo-blood group antigens revealed by retagging. Science 279, 373–377.[CrossRef]
    [Google Scholar]
  22. Kamisago, S., Iwamori, M., Tai, T., Mitamura, K., Yazaki, Y. & Sugano, K. ( 1996;). Role of sulfatides in adhesion of Helicobacter pylori to gastric cancer cells. Infect Immun 64, 624–628.
    [Google Scholar]
  23. Klein, P., Somorjai, R. L. & Lau, P. C. ( 1988;). Distinctive properties of signal sequences from bacterial lipoproteins. Protein Eng 2, 15–20.[CrossRef]
    [Google Scholar]
  24. Lee, A., Fox, J. & Hazell, S. ( 1993;). Pathogenicity of Helicobacter pylori: a perspective. Infect Immun 61, 1601–1610.
    [Google Scholar]
  25. Logan, R. P. ( 1994;). Helicobacter pylori and gastric cancer. Lancet 344, 1078–1079.
    [Google Scholar]
  26. Mori, N., Wada, A., Hirayama, T., Parks, T. P., Stratowa, C. & Yamamoto, N. ( 2000;). Activation of intercellular adhesion molecule 1 expression by Helicobacter pylori is regulated by NF-kappaB in gastric epithelial cancer cells. Infect Immun 68, 1806–1814.[CrossRef]
    [Google Scholar]
  27. Nishihara, K., Nozawa, Y., Nomura, S., Kitazato, K. & Miyake, H. ( 1999;). Analysis of Helicobacter pylori binding site on HEp-2 cells and three cell lines from human gastric carcinoma. Fundam Clin Pharmacol 13, 555–561.[CrossRef]
    [Google Scholar]
  28. Odenbreit, S., Till, M., Hofreuter, D., Faller, G. & Haas, R. ( 1999;). Genetic and functional characterization of the alpAB gene locus essential for the adhesion of Helicobacter pylori to human gastric tissue. Mol Microbiol 31, 1537–1548.[CrossRef]
    [Google Scholar]
  29. Palovuori, R., Perttu, A., Yan, Y., Karttunen, R., Eskelinen, S. & Karttunen, T. J. ( 2000;). Helicobacter pylori induces formation of stress fibers and membrane ruffles in AGS cells by rac activation. Biochem Biophys Res Commun 269, 247–253.[CrossRef]
    [Google Scholar]
  30. Peek, R. M., Jr, Miller, G. G., Tham, K. T., Perez-Perez, G. I., Zhao, X., Atherton, J. C. & Blaser, M. J. ( 1995;). Heightened inflammatory response and cytokine expression in vivo to cagA+ Helicobacter pylori strains. Lab Invest 73, 760–770.
    [Google Scholar]
  31. Pride, D. T., Meinersmann, R. J. & Blaser, M. J. ( 2001;). Allelic variation within Helicobacter pylori babA and babB. Infect Immun 69, 1160–1171.[CrossRef]
    [Google Scholar]
  32. Saitoh, T., Natomi, H., Zhao, W. L., Okuzumi, K., Sugano, K., Iwamori, M. & Nagai, Y. ( 1991;). Identification of glycolipid receptors for Helicobacter pylori by TLC-immunostaining. FEBS Lett 282, 385–387.[CrossRef]
    [Google Scholar]
  33. Soltesz, V., Zeeberg, B. & Wadstrom, T. ( 1992;). Optimal survival of Helicobacter pylori under various transport conditions. J Clin Microbiol 30, 1453–1456.
    [Google Scholar]
  34. Su, B., Hellstrom, P. M., Rubio, C., Celik, J., Granstrom, M. & Normark, S. ( 1998;). Type I Helicobacter pylori shows Lewis(b)-independent adherence to gastric cells requiring de novo protein synthesis in both host and bacteria. J Infect Dis 178, 1379–1390.[CrossRef]
    [Google Scholar]
  35. Svanborg, C., Godaly, G. & Hedlund, M. ( 1999;). Cytokine responses during mucosal infections: role in disease pathogenesis and host defence. Curr Opin Microbiol 2, 99–105.[CrossRef]
    [Google Scholar]
  36. Thomsen, L. L., Gavin, J. B. & Tasman-Jones, C. ( 1990;). Relation of Helicobacter pylori to the human gastric mucosa in chronic gastritis of the antrum. Gut 31, 1230–1236.[CrossRef]
    [Google Scholar]
  37. Towler, D. A., Adams, S. P., Eubanks, S. R., Towery, D. S., Jackson-Machelski, E., Glaser, L. & Gordon, J. I. ( 1988;). Myristoyl CoA:protein N-myristoyltransferase activities from rat liver and yeast possess overlapping yet distinct peptide substrate specificities. J Biol Chem 263, 1784–1790.
    [Google Scholar]
  38. Wadstrom, T., Hirmo, S. & Boren, T. ( 1996;). Biochemical aspects of Helicobacter pylori colonization of the human gastric mucosa. Aliment Pharmacol Ther 10 Suppl 1, 17–27.
    [Google Scholar]
  39. Wadstrom, T., Hirmo, S. & Nilsson, B. ( 1997;). Biochemical aspects of H.pylori adhesion. J Physiol Pharmacol 48, 325–331.
    [Google Scholar]
  40. Warren, H. P. & Marshall, B. J. ( 1983;). Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet 1, 1273–1275.
    [Google Scholar]
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