assessment of the APTIMA Combo 2 assay for the detection of using highly purified elementary bodies Free

Abstract

The Gen-Probe APTIMA Combo 2 assay has previously been reported to have a high sensitivity. The end point of this assay was evaluated using highly purified chlamydial elementary bodies (EBs). The performance of the APTIMA Combo 2 assay was compared with a commercially available PCR kit, AMPLICOR . The number of inclusions of at the end point of the APTIMA Combo 2 assay was 0.005 inclusion-forming units (i.f.u.) ml, which was equivalent to 0.008 EBs per assay. The end point of the AMPLICOR kit was 5 i.f.u. ml (equivalent to 0.5 EB per assay). The efficacy of the AMPLICOR assay was inhibited by phosphate or Fe ion, while these had no effect on the APTIMA Combo 2 assay. In conclusion, the APTIMA Combo 2 assay appears to have a greater sensitivity than the AMPLICOR assay for detection of , while demonstrating few problems with inhibitory substances such as phosphate and Fe ion.

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2005-04-01
2024-03-28
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