1887

Abstract

The Gen-Probe APTIMA Combo 2 assay has previously been reported to have a high sensitivity. The end point of this assay was evaluated using highly purified chlamydial elementary bodies (EBs). The performance of the APTIMA Combo 2 assay was compared with a commercially available PCR kit, AMPLICOR . The number of inclusions of at the end point of the APTIMA Combo 2 assay was 0.005 inclusion-forming units (i.f.u.) ml, which was equivalent to 0.008 EBs per assay. The end point of the AMPLICOR kit was 5 i.f.u. ml (equivalent to 0.5 EB per assay). The efficacy of the AMPLICOR assay was inhibited by phosphate or Fe ion, while these had no effect on the APTIMA Combo 2 assay. In conclusion, the APTIMA Combo 2 assay appears to have a greater sensitivity than the AMPLICOR assay for detection of , while demonstrating few problems with inhibitory substances such as phosphate and Fe ion.

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2005-04-01
2021-10-16
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References

  1. Bass C. A., Jungkind D. L., Silverman N. S., Bondi J. M. 1993; Clinical evaluation of a new polymerase chain reaction assay for detection of Chlamydia trachomatis in endocervical specimens. J Clin Microbiol 31:2648–2653
    [Google Scholar]
  2. Bauwens J. E., Clark A. M., Stamm W. E. 1993; Diagnosis of Chlamydia trachomatis endocervical infections by a commercial polymerase chain reaction assay. J Clin Microbiol 31:3023–3027
    [Google Scholar]
  3. Beatty W. L., Morrison R. P., Byrne G. I. 1994; Persistent chlamydiae: from cell culture to a paradigm for chlamydial pathogenesis. Microbiol Rev 58:686–699
    [Google Scholar]
  4. Beatty W. L., Morrison R. P., Byrne G. I. 1995; Reactivation of persistent Chlamydia trachomatis infection in cell culture. Infect Immun 63:199–205
    [Google Scholar]
  5. Cates W. Jr, Wasserheit J. N. 1991; Genital chlamydial infections: epidemiology and reproductive sequelae. Am J Obstet Gynecol 164:1771–1781 [CrossRef]
    [Google Scholar]
  6. Chong S., Jang D., Song X., Mahony J., Petrich A., Barriga P., Chernesky M. 2003; Specimen processing and concentration of Chlamydia trachomatis added can influence false-negative rates in the LCx assay but not in the APTIMA Combo 2 assay when testing for inhibitors. J Clin Microbiol 41:778–782 [CrossRef]
    [Google Scholar]
  7. de Barbeyrac B., Rodriguez P., Dutilh B., Le Roux P., Bebear C. 1995; Detection of Chlamydia trachomatis by ligase chain reaction compared with polymerase chain reaction and cell culture in urogenital specimens. Genitourin Med 71:382–386
    [Google Scholar]
  8. Gaston J. S. 1995; Reactive arthritis. Rheumatol Eur 24:5–22
    [Google Scholar]
  9. Gaydos C. A., Quinn T. C., Willis D., Weissfeld A., Hook E. W., Martin D. H., Ferreo D. V., Schachter J. 2003; Performance of the APTIMA Combo 2 assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in female urine and endocervical swab specimens. J Clin Microbiol 41:304–309 [CrossRef]
    [Google Scholar]
  10. Goessens W. H. F., Mouton J. W., van der Meijden W. I., Deelen S., Rijsoort-Vos T. H., Lemmens-den Toom N., Verbrugh H. A., Verkooyen R. P. 1997; Comparison of three commercially available amplification assays, AMP CT, LCx, and COBAS AMPLICOR, for detection of Chlamydia trachomatis in first-void urine. J Clin Microbiol 35:2628–2633
    [Google Scholar]
  11. Hanada H., Ikeda-Dantsuji Y., Naito M., Nagayama A. 2003; Infection of human fibroblast-like synovial cells with Chlamydia trachomatis results in persistent infection and interleukin-6 production. Microb Pathog 34:57–63 [CrossRef]
    [Google Scholar]
  12. Hogan R. J., Mathews S. A., Mukhopadhyay S., Summersgill J. T., Timms P. 2004; Chlamydial persistence: beyond the biphasic paradigm. Infect Immun 72:1843–1855 [CrossRef]
    [Google Scholar]
  13. Jensen I. P., Thorsen P., Moller B. R. 1997; Sensitivity of ligase chain reaction assay of urine from pregnant women for Chlamydia trachomatis . Lancet 349:329–330
    [Google Scholar]
  14. Mahony J. B., Chong S., Jang D., Luinstra K., Faught M., Dalby D., Sellors J., Chernesky M. 1998; Urine specimens from pregnant and nonpregnant women inhibitory to amplification of Chlamydia trachomatis nucleic acid by PCR, ligase chain reaction, and transcription-mediated amplification: identification of urinary substances associated with inhibition and removal of inhibitory activity. J Clin Microbiol 36:3122–3126
    [Google Scholar]
  15. Miyashita N., Matsumoto A. 1992; Establishment of a particle-counting method for purified elementary bodies of chlamydiae and evaluation of sensitivities of the IDEIA Chlamydia kit and DNA probe by using the purified elementary bodies. J Clin Microbiol 30:2911–2916
    [Google Scholar]
  16. Miyashita N., Matsumoto A., Niki Y., Matsushima T. 1996; Evaluation of the sensitivity and specificity of a ligase chain reaction test kit for the detection of Chlamydia trachomatis . J Clin Pathol 49:515–517 [CrossRef]
    [Google Scholar]
  17. Nagayama A., Nakao T., Taen H. 1988; In vitro activities of ofloxacin and four other new quinolone-carboxylic acids against Chlamydia trachomatis . Antimicrob Agents Chemother 32:1735–1737 [CrossRef]
    [Google Scholar]
  18. Notomi T., Ikeda Y., Okadome A., Nagayama A. 1998; The inhibitory effect of phosphate on the ligase chain reaction used for detecting Chlamydia trachomatis . J Clin Pathol 51:306–308 [CrossRef]
    [Google Scholar]
  19. Okadome A., Notomi T., Nomura S., Nagayama A. 1999; Reactivity of a dual amplified chlamydia immunoassay with different serovars of Chlamydia trachomatis . Int J STD AIDS 10:460–463 [CrossRef]
    [Google Scholar]
  20. Peterson E. M., Darrow V., Blanding J., Aarnaes S., de la Maza L. M. 1997; Reproducibility problems with the AMPLICOR PCR Chlamydia trachomatis test. J Clin Microbiol 35:957–959
    [Google Scholar]
  21. Rahaman M. U., Cheema M. A., Schumacher H. R., Hudson A. P. 1992; Molecular evidence for the presence of chlamydia in the synovium of patients with Reiter's syndrome. Arthritis Rheum 35:521–529 [CrossRef]
    [Google Scholar]
  22. Toye B., Woods W., Bobrowska M., Ramotar K. 1998; Inhibition of PCR in genital and urine specimens submitted for Chlamydia trachomatis testing. J Clin Microbiol 36:2356–2358
    [Google Scholar]
  23. Verkooyen R. P., Luijendijk A., Huisman W. M., Goessens W. H. F., Kluytmans J. A. J. W., van Rijsoort-Vos J. H., Verbrugh H. A. 1996; Detection of PCR inhibitors in cervical specimens by using the AMPLICOR Chlamydia trachomatis assay. J Clin Microbiol 34:3072–3074
    [Google Scholar]
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