1887

Abstract

The Gen-Probe APTIMA Combo 2 assay has previously been reported to have a high sensitivity. The end point of this assay was evaluated using highly purified chlamydial elementary bodies (EBs). The performance of the APTIMA Combo 2 assay was compared with a commercially available PCR kit, AMPLICOR . The number of inclusions of at the end point of the APTIMA Combo 2 assay was 0.005 inclusion-forming units (i.f.u.) ml, which was equivalent to 0.008 EBs per assay. The end point of the AMPLICOR kit was 5 i.f.u. ml (equivalent to 0.5 EB per assay). The efficacy of the AMPLICOR assay was inhibited by phosphate or Fe ion, while these had no effect on the APTIMA Combo 2 assay. In conclusion, the APTIMA Combo 2 assay appears to have a greater sensitivity than the AMPLICOR assay for detection of , while demonstrating few problems with inhibitory substances such as phosphate and Fe ion.

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2005-04-01
2019-11-18
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References

  1. Bass, C. A., Jungkind, D. L., Silverman, N. S. & Bondi, J. M. ( 1993;). Clinical evaluation of a new polymerase chain reaction assay for detection of Chlamydia trachomatis in endocervical specimens. J Clin Microbiol 31, 2648–2653.
    [Google Scholar]
  2. Bauwens, J. E., Clark, A. M. & Stamm, W. E. ( 1993;). Diagnosis of Chlamydia trachomatis endocervical infections by a commercial polymerase chain reaction assay. J Clin Microbiol 31, 3023–3027.
    [Google Scholar]
  3. Beatty, W. L., Morrison, R. P. & Byrne, G. I. ( 1994;). Persistent chlamydiae: from cell culture to a paradigm for chlamydial pathogenesis. Microbiol Rev 58, 686–699.
    [Google Scholar]
  4. Beatty, W. L., Morrison, R. P. & Byrne, G. I. ( 1995;). Reactivation of persistent Chlamydia trachomatis infection in cell culture. Infect Immun 63, 199–205.
    [Google Scholar]
  5. Cates, W., Jr & Wasserheit, J. N. ( 1991;). Genital chlamydial infections: epidemiology and reproductive sequelae. Am J Obstet Gynecol 164, 1771–1781.[CrossRef]
    [Google Scholar]
  6. Chong, S., Jang, D., Song, X., Mahony, J., Petrich, A., Barriga, P. & Chernesky, M. ( 2003;). Specimen processing and concentration of Chlamydia trachomatis added can influence false-negative rates in the LCx assay but not in the APTIMA Combo 2 assay when testing for inhibitors. J Clin Microbiol 41, 778–782.[CrossRef]
    [Google Scholar]
  7. de Barbeyrac, B., Rodriguez, P., Dutilh, B., Le Roux, P. & Bebear, C. ( 1995;). Detection of Chlamydia trachomatis by ligase chain reaction compared with polymerase chain reaction and cell culture in urogenital specimens. Genitourin Med 71, 382–386.
    [Google Scholar]
  8. Gaston, J. S. ( 1995;). Reactive arthritis. Rheumatol Eur 24, 5–22.
    [Google Scholar]
  9. Gaydos, C. A., Quinn, T. C., Willis, D., Weissfeld, A., Hook, E. W., Martin, D. H., Ferreo, D. V. & Schachter, J. ( 2003;). Performance of the APTIMA Combo 2 assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in female urine and endocervical swab specimens. J Clin Microbiol 41, 304–309.[CrossRef]
    [Google Scholar]
  10. Goessens, W. H. F., Mouton, J. W., van der Meijden, W. I., Deelen, S., Rijsoort-Vos, T. H., Lemmens-den Toom, N., Verbrugh, H. A. & Verkooyen, R. P. ( 1997;). Comparison of three commercially available amplification assays, AMP CT, LCx, and COBAS AMPLICOR, for detection of Chlamydia trachomatis in first-void urine. J Clin Microbiol 35, 2628–2633.
    [Google Scholar]
  11. Hanada, H., Ikeda-Dantsuji, Y., Naito, M. & Nagayama, A. ( 2003;). Infection of human fibroblast-like synovial cells with Chlamydia trachomatis results in persistent infection and interleukin-6 production. Microb Pathog 34, 57–63.[CrossRef]
    [Google Scholar]
  12. Hogan, R. J., Mathews, S. A., Mukhopadhyay, S., Summersgill, J. T. & Timms, P. ( 2004;). Chlamydial persistence: beyond the biphasic paradigm. Infect Immun 72, 1843–1855.[CrossRef]
    [Google Scholar]
  13. Jensen, I. P., Thorsen, P. & Moller, B. R. ( 1997;). Sensitivity of ligase chain reaction assay of urine from pregnant women for Chlamydia trachomatis. Lancet 349, 329–330.
    [Google Scholar]
  14. Mahony, J. B., Chong, S., Jang, D., Luinstra, K., Faught, M., Dalby, D., Sellors, J. & Chernesky, M. ( 1998;). Urine specimens from pregnant and nonpregnant women inhibitory to amplification of Chlamydia trachomatis nucleic acid by PCR, ligase chain reaction, and transcription-mediated amplification: identification of urinary substances associated with inhibition and removal of inhibitory activity. J Clin Microbiol 36, 3122–3126.
    [Google Scholar]
  15. Miyashita, N. & Matsumoto, A. ( 1992;). Establishment of a particle-counting method for purified elementary bodies of chlamydiae and evaluation of sensitivities of the IDEIA Chlamydia kit and DNA probe by using the purified elementary bodies. J Clin Microbiol 30, 2911–2916.
    [Google Scholar]
  16. Miyashita, N., Matsumoto, A., Niki, Y. & Matsushima, T. ( 1996;). Evaluation of the sensitivity and specificity of a ligase chain reaction test kit for the detection of Chlamydia trachomatis. J Clin Pathol 49, 515–517.[CrossRef]
    [Google Scholar]
  17. Nagayama, A., Nakao, T. & Taen, H. ( 1988;). In vitro activities of ofloxacin and four other new quinolone-carboxylic acids against Chlamydia trachomatis. Antimicrob Agents Chemother 32, 1735–1737.[CrossRef]
    [Google Scholar]
  18. Notomi, T., Ikeda, Y., Okadome, A. & Nagayama, A. ( 1998;). The inhibitory effect of phosphate on the ligase chain reaction used for detecting Chlamydia trachomatis. J Clin Pathol 51, 306–308.[CrossRef]
    [Google Scholar]
  19. Okadome, A., Notomi, T., Nomura, S. & Nagayama, A. ( 1999;). Reactivity of a dual amplified chlamydia immunoassay with different serovars of Chlamydia trachomatis. Int J STD AIDS 10, 460–463.[CrossRef]
    [Google Scholar]
  20. Peterson, E. M., Darrow, V., Blanding, J., Aarnaes, S. & de la Maza, L. M. ( 1997;). Reproducibility problems with the AMPLICOR PCR Chlamydia trachomatis test. J Clin Microbiol 35, 957–959.
    [Google Scholar]
  21. Rahaman, M. U., Cheema, M. A., Schumacher, H. R. & Hudson, A. P. ( 1992;). Molecular evidence for the presence of chlamydia in the synovium of patients with Reiter's syndrome. Arthritis Rheum 35, 521–529.[CrossRef]
    [Google Scholar]
  22. Toye, B., Woods, W., Bobrowska, M. & Ramotar, K. ( 1998;). Inhibition of PCR in genital and urine specimens submitted for Chlamydia trachomatis testing. J Clin Microbiol 36, 2356–2358.
    [Google Scholar]
  23. Verkooyen, R. P., Luijendijk, A., Huisman, W. M., Goessens, W. H. F., Kluytmans, J. A. J. W., van Rijsoort-Vos, J. H. & Verbrugh, H. A. ( 1996;). Detection of PCR inhibitors in cervical specimens by using the AMPLICOR Chlamydia trachomatis assay. J Clin Microbiol 34, 3072–3074.
    [Google Scholar]
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