1887

Abstract

During an outbreak of legionellosis in Belgium, urine samples of 32 legionellosis patients were tested with three urinary antigen assays: the Biotest enzyme immunoassay (EIA) kit, the Binax EIA kit and the Binax NOW Immunochromatographic Test kit. The three tests were concomitantly compared. The test sensitivities on the first urine samples were 65.6 % for the Biotest EIA, 50.0 % for the Binax EIA and 56.3 % for the Binax NOW. Testing of a second urine sample increased the sensitivities to 71.9 %, 59.4 % and 65.6 %, respectively. The differences were not statistically significant. In outbreak settings, testing second samples from patients presenting with symptoms but initially testing negative and/or concentrating urine samples for testing might be valuable additions to the urinary antigen test to increase the sensitivities of the tests.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.45909-0
2005-12-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/jmm/54/12/JM541214.html?itemId=/content/journal/jmm/10.1099/jmm.0.45909-0&mimeType=html&fmt=ahah

References

  1. Ballard A. L., Fry N. K., Chan L., Surman S. B., Lee J. V., Harrison T. G., Towner K. J. 2000; Detection of Legionella pneumophila using a real-time PCR hybridization assay. J Clin Microbiol 38:4215–4218
    [Google Scholar]
  2. Benson R. F., Tang P. W., Fields B. S. 2000; Evaluation of the Binax and the Biotest urinary antigen kits for detection of Legionnaires’ disease due to multiple serogroups and species of Legionella . J Clin Microbiol 38:2763–2765
    [Google Scholar]
  3. Breiman R. F., Butler J. C. 1998; Legionnaires’ disease: clinical, epidemiological, and public health perspectives. Semin Respir Infect 13:84–89
    [Google Scholar]
  4. Cloud J. L., Carroll K. C., Pixton P., Erali M., Hillyard D. R. 2000; Detection of Legionella species in respiratory specimens using PCR with sequencing confirmation. J Clin Microbiol 38:1709–1712
    [Google Scholar]
  5. De Schrijver K., Van Bouwel E., Mortelmans L. 7 other authors; 2000; An outbreak of Legionnaires’ disease among visitors to a fair in Belgium in 1999. Eurosurveillance 5:115–119
    [Google Scholar]
  6. De Schrijver K., Dirven K., Van Bouwel K. & 11 other authors; 2003; An outbreak of Legionnaires’ disease among visitors to a fair in Belgium in 1999. Public Health 117:117–124 [CrossRef]
    [Google Scholar]
  7. Domínguez J. A., Manterola J. M., Blavia R. & 7 other authors; 1996; Detection of Legionella pneumophila serogroup 1 antigen in nonconcentrated urine and urine concentrated by selective ultrafiltration. J Clin Microbiol 34:2334–2336
    [Google Scholar]
  8. Domínguez J. A., Galí N., Pedroso P., Fargas A., Padilla E., Manterola J. M., Matas L. 1998; Comparison of the Binax Legionella urinary antigen enzyme immunoassay (EIA) with the Biotest Legionella urine antigen EIA for detection of Legionella antigen in both concentrated and nonconcentrated urine samples. J Clin Microbiol 36:2718–2722
    [Google Scholar]
  9. Domínguez J., Galí N., Matas L., Pedroso P., Hernández A., Padilla E., Ausina V. 1999; Evaluation of a rapid immunochromatographic assay for the detection of Legionella antigen in urine samples. Eur J Clin Microbiol Infect Dis 18:896–898 [CrossRef]
    [Google Scholar]
  10. Edelstein P. H. 1993; Legionnaires’ disease. Clin Infect Dis 16:741–749 [CrossRef]
    [Google Scholar]
  11. Fehrenbach F. J., Fuhrmann B., Horbach J., Jürgens D. 1995; Laboratory diagnosis of Legionnaires’ disease (LD) 19 years after Philadelphia. Biotest Bulletin 5:191–196
    [Google Scholar]
  12. Harrison T., Uldum S., Alexiou-Daniel S. & 12 other authors; 1998; A multicenter evaluation of the Biotest Legionella urinary antigen assay. Clin Microbiol Infect 4:359–365 [CrossRef]
    [Google Scholar]
  13. Helbig J. H., Uldum S. A., Lück P. C., Harrison T. G. 2001; Detection of Legionella pneumophila antigen in urine samples by the Binax Now immunochromatographic assay and comparison with both Binax Legionella Urinary Enzyme Immunoassay (EIA) and Biotest Legionella Urin Antigen EIA. J Med Microbiol 50:509–516
    [Google Scholar]
  14. Helbig J. H., Uldum S. A., Bernander S., Lück P. C., Wewalka G., Abraham B., Gaia V., Harrison T. G. 2003; Clinical utility of urinary antigen detection for diagnosis of community-acquired, travel- associated, and nosocomial Legionnaires’ disease. J Clin Microbiol 41:838–840 [CrossRef]
    [Google Scholar]
  15. Horn J. 2001; Binax and Biotest Legionella urinary antigen kits. J Clin Microbiol 39:1682 [CrossRef]
    [Google Scholar]
  16. Kashuba A. D. M., Ballow C. H. 1996; Legionella urinary antigen testing: potential impact on diagnosis and antibiotic therapy. Diagn Microbiol Infect Dis 24:129–139 [CrossRef]
    [Google Scholar]
  17. Kazandjian D., Chiew R., Gilbert G. L. 1997; Rapid diagnosis of Legionella pneumophila serogroup 1 infection with the Binax enzyme immunoassay urinary antigen test. J Clin Microbiol 35:954–956
    [Google Scholar]
  18. Marston B. J., Lipman H. B., Breiman R. F. 1994; Surveillance for Legionnaires’ disease: risk factors for morbidity and mortality. Arch Intern Med 154:22417–22422
    [Google Scholar]
  19. Ruf B., Schürmann D., Horbach I., Fehrenbach F. J., Pohle H. D. 1990; Prevalence and diagnosis of Legionella pneumonia: a 3-year prospective study with emphasis on application of urinary antigen detection. J Infect Dis 162:1341–1348 [CrossRef]
    [Google Scholar]
  20. Stout J. E., Yu V. L. 1997; Legionellosis. N Engl J Med 337:682–687 [CrossRef]
    [Google Scholar]
  21. Templeton K. E., Scheltinga S. A., Sillekens P., Crielaard J. W., van Dam A. P., Goossens H., Claass E. C. J. 2003; Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species. J Clin Microbiol 41:4016–4021 [CrossRef]
    [Google Scholar]
  22. Waterer G. W., Baselski V. S., Wunderink R. G. 2001; Legionella and community-acquired pneumonia: a review of current diagnostic tests from a clinician's viewpoint. Am J Med 110:41–48 [CrossRef]
    [Google Scholar]
  23. Welti M., Jaton K., Altwegg M., Sahli R., Wenger A., Bille J. 2003; Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions. Diagn Microbiol Infect Dis 45:85–95 [CrossRef]
    [Google Scholar]
  24. Wever P. C., Yzerman E. P. F., Kuijper E. J., Speelman P., Dankert J. 2000; Rapid diagnosis of Legionnaires’ disease using an immunochromatographic assay for Legionella pneumophila serogroup 1 antigen in urine during an outbreak in The Netherlands. J Clin Microbiol 38:2738–2739
    [Google Scholar]
  25. Wilson D., Yen-Lieberman B., Reischl U., Gordon S. M., Procop G. W. 2003; Detection of Legionella pneumophila by real-time PCR for the mip gene. J Clin Microbiol 41:3327–3330 [CrossRef]
    [Google Scholar]
  26. Yzerman E. P. F., den Boer J. W., Lettinga K. D., Schellekens J., Dankert J., Peeters M. 2002; Sensitivity of three urinary antigen tests associated with clinical severity in a large outbreak of Legionnaires’ disease in The Netherlands. J Clin Microbiol 40:3232–3236 [CrossRef]
    [Google Scholar]
/content/journal/jmm/10.1099/jmm.0.45909-0
Loading
/content/journal/jmm/10.1099/jmm.0.45909-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error