1887

Abstract

Enteroinvasive (EIEC) O164 strain RIMD05091045 was isolated from a travelling patient suffering from diarrhoea at the Osaka airport quarantine facility in Japan. The strain showed multidrug resistance against streptomycin, spectinomycin, co-trimoxazole (trimethoprim/sulfamethoxazole) and ampicillin, and reduced susceptibility to ciprofloxacin. Molecular characterization of the multidrug-resistance phenotype revealed the presence of a class 1 integron containing three genes, a dihydrofolate reductase type XII gene, , which confers resistance to trimethoprim, an aminoglycoside adenyltransferase gene, , which confers resistance to streptomycin and spectinomycin, and an ORF of unknown function. Southern blot hybridization and conjugation experiments showed that the class 1 integron was located on a transferable plasmid that was less than 90 kb in size. The resistance of EIEC O164 to ampicillin was found to be due to the presence of TEM-1 β-lactamase. On the other hand, a single mutation that has not previously been described, P158-to-S, was detected downstream of the quinolone-resistance-determining region of of topoisomerase IV and may be responsible for the reduced susceptibility to ciprofloxacin in this strain.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.45908-0
2005-03-01
2020-09-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/54/3/JM540309.html?itemId=/content/journal/jmm/10.1099/jmm.0.45908-0&mimeType=html&fmt=ahah

References

  1. Ahmed A. M., Shimamoto T. 2004; A plasmid-encoded class 1 integron carrying sat , a putative phosphoserine phosphatase gene and aadA2 from enterotoxigenic Escherichia coli O159 isolated in Japan. FEMS Microbiol Lett 235:243–248 [CrossRef]
    [Google Scholar]
  2. Bebear C. M., Renaudin H., Charron A., Clerc M., Pereyre S., Bebear C. 2003; DNA gyrase and topoisomerase IV mutations in clinical isolates of Ureaplasma spp.and Mycoplasma hominis resistant to fluoroquinolones. Antimicrob Agents Chemother 47:3323–3325 [CrossRef]
    [Google Scholar]
  3. Bopp C. A., Brenner F. W., Wells J. G., Stockbine N. A. 1999; Escherichia , Shigella , and Salmonella . In Manual of Clinical Microbiology , 7th edn. pp 459–474 Edited by Murray P. R. and others Washington, DC: American Society for Microbiology;
    [Google Scholar]
  4. Bradford P. A. 2001; Extended-spectrum β-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev 14:933–951 [CrossRef]
    [Google Scholar]
  5. Chaudhry U., Ray K., Bala M., Saluja D. 2002; Mutation patterns in gyrA and parC genes of ciprofloxacin resistant isolates of Neisseria gonorrhoeae from India. Sex Transm Infect 78:440–444 [CrossRef]
    [Google Scholar]
  6. Fendukly F., Karlsson I., Hanson H. S., Kronvall G., Dornbusch K. 2003; Patterns of mutations in target genes in septicemia isolates of Escherichia coli and Klebsiella pneumoniae with resistance or reduced susceptibility to ciprofloxacin. APMIS 111:857–866 [CrossRef]
    [Google Scholar]
  7. Fournier B., Hooper D. C. 1998; Mutations in topoisomerase IV and DNA gyrase of Staphylococcus aureus : novel pleiotropic effects on quinolone and coumarin activity. Antimicrob Agents Chemother 42:121–128 [CrossRef]
    [Google Scholar]
  8. Gassama A., Aidara-Kane A., Chainier D., Denis F., Ploy M. C. 2004; Integron-associated antibiotic resistance in enteroaggregative and enteroinvasive Escherichia coli . Microb Drug Resist 10:27–30 [CrossRef]
    [Google Scholar]
  9. Harris J. R., Mariano J., Wells J. G., Payne B. J., Donnell H. D., Cohen M. L. 1985; Person-to-person transmission in an outbreak of enteroinvasive Escherichia coli . Am J Epidemiol 122:245–252
    [Google Scholar]
  10. Heisig P. 1996; Genetic evidence for a role of parC mutations in development of high-level fluoroquinolone resistance in Escherichia coli . Antimicrob Agents Chemother 40:879–885
    [Google Scholar]
  11. Ince D., Hooper D. C. 2000; Mechanisms and frequency of resistance to premafloxacin in Staphylococcus aureus : novel mutations suggest novel drug-target interactions. Antimicrob Agents Chemother 44:3344–3350 [CrossRef]
    [Google Scholar]
  12. Jones M. E., Peters E., Weersink A. M., Fluit A., Verhoef J. 1997; Widespread occurrence of integrons causing multiple antibiotic resistances in bacteria. Lancet 349:1742–1743
    [Google Scholar]
  13. Lévesque C., Piché L., Larose C., Roy P. H. 1995; PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob Agents Chemother 39:185–191 [CrossRef]
    [Google Scholar]
  14. Livermore D. M. 1995; β-Lactamases in laboratory and clinical resistance. Clin Microbiol Rev 8:557–584
    [Google Scholar]
  15. Marier R., Wells J. G., Swanson R. C., Callahan W., Mehlman I. J. 1973; An outbreak of enteropathogenic Escherichia coli foodborne disease traced to imported French cheese. Lancet 2:1376–1378
    [Google Scholar]
  16. Maynard C., Fairbrother J. M., Bekal S., Sanschagrin F., Levesque R. C., Brousseau R., Masson L., Larivière S., Harel J. 2003; Antimicrobial resistance genes in enterotoxigenic Escherichia coli O149: K91 isolates obtained over a 23-year period from pigs. Antimicrob Agents Chemother 47:3214–3221 [CrossRef]
    [Google Scholar]
  17. Nataro J. P., Kaper J. B. 1998; Diarrheagenic Escherichia coli . Clin Microbiol Rev 11:142–201
    [Google Scholar]
  18. NCCLS 2002 Performance Standards for Antimicrobial Susceptibility Testing , 12th Informational Supplement, Approved Standard M100-S12, NCCLS Wayne, PA: National Committee for Clinical Laboratory Standards;
    [Google Scholar]
  19. Orskov F., Orskov I. 1984; Serotyping of Escherichia coli . Methods Microbiol 14:43–112
    [Google Scholar]
  20. Piddock L. J. V. 1999; Mechanisms of fluoroquinolone resistance: an update 1994–1998. Drugs 58:11–18
    [Google Scholar]
  21. Rowe-Magnus D. A., Mazel D. 2001; Integrons: natural tools for bacterial genome evolution. Curr Opin Microbiol 4:565–569 [CrossRef]
    [Google Scholar]
  22. Rowe-Magnus D. A., Guerout A. M., Mazel D. 2002; Bacterial resistance evolution by recruitment of super-integron gene cassettes. Mol Microbiol 43:1657–1669 [CrossRef]
    [Google Scholar]
  23. Sambrook J., Russell D. 2001 Molecular Cloning: a Laboratory Manual , 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Snyder J. D., Wells J. G., Yashuk J., Puhr N., Blake P. A. 1984; Outbreak of invasive Escherichia coli gastroenteritis on a cruise ship. Am J Trop Med Hyg 33:281–284
    [Google Scholar]
  25. Taylor D. N., Echeverria P., Pal T., Sethabutr O., Sailborisuth S., Sricharmom S., Rowe B., Cross J. 1986; The role of Shigella ssp.enteroinvasive Escherichia coli , and other enteropathogens as causes of childhood dysentery in Thailand. J Infect Dis 153:1131–1138
    [Google Scholar]
  26. Todorova K., Bratoeva M., Daneva M. 1990; Characterization of enteroinvasive Escherichia coli serotype O164 by means of plasmid analysis and virulence assay. J Basic Microbiol 30:451–454 [CrossRef]
    [Google Scholar]
  27. Toma C., Lu Y., Higa N., Nakasone N., Chinen I., Baschkier A., Rivas M., Iwanaga M. 2003; Multiplex PCR assay for identification of human diarrheagenic Escherichia coli . J Clin Microbiol 41:2669–2671 [CrossRef]
    [Google Scholar]
  28. Trees D. L., Sandul A. L., Whittington W. L., Knapp J. S. 1998; Identification of novel mutation patterns in the parC gene of ciprofloxacin-resistant isolates of Neisseria gonorrhoeae . Antimicrob Agents Chemother 42:2103–2105
    [Google Scholar]
  29. Webber M. A., Piddock L. J. V. 2001; Absence of mutations in marRAB or soxRS in acrB -overexpressing fluoroquinolone-resistant clinical and veterinary isolates of Escherichia coli . Antimicrob Agents Chemother 45:1550–1552 [CrossRef]
    [Google Scholar]
  30. Webber M. A., Piddock L. J. V. 2003; The importance of efflux pumps in bacterial antibiotic resistance. J Antimicrob Chemother 51:9–11 [CrossRef]
    [Google Scholar]
  31. Weill F.-X., Demartin M., Tande D., Espie E., Rakotoarivony I., Grimont P. A. D. 2004; SHV-12-like extended-spectrum-β-lactamase-producing strains of Salmonella enterica serotypes Babelsberg and Enteritidis isolated in France among infants adopted from Mali. J Clin Microbiol 42:2432–2437 [CrossRef]
    [Google Scholar]
  32. WHO 1995 Guidelines for the Control of Epidemics due to Shigella dysenteriae 1 Geneva: World Health Organization, Publication; no. WHO/CDR/95.4
    [Google Scholar]
  33. Yamamura K., Sumi N., Egashira Y., Fukuoka I., Motomura S., Tsuchida R. 1992; Food poisoning caused by enteroinvasive Escherichia coli (O164: H-) - a case in which the causative agent was identified (Japanese). J Jpn Assoc Infect Dis 66:761–768
    [Google Scholar]
  34. Zhao S., White D. G., Ge B., Ayers S., Friedman S., English L., Wagner D., Gaines S., Meng J. 2001; Identification and characterization of integron-mediated antibiotic resistance among shiga toxin-producing Escherichia coli isolates. Appl Environ Microbiol 67:1558–1564 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.45908-0
Loading
/content/journal/jmm/10.1099/jmm.0.45908-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error