Sample type is crucial to the diagnosis of pneumonia by PCR Free

Abstract

Sensitive and specific methods for rapid laboratory diagnosis of were not available until nucleic acid amplification methods were developed. The choice of sample type and method of sampling are crucial to optimal diagnostic efficacy. Three types of respiratory samples from 32 young military conscripts with pneumonia were collected during an outbreak of infection. Sputum, nasopharyngeal aspirate and throat swab specimens were tested by 16S rRNA gene-based PCR with liquid-phase probe hybridization, and the results were compared with serology. The PCR result was positive for 22 (69 %) of the sputa, 16 (50 %) of the aspirates and 12 (37.5 %) of the swabs. Serology with increasing or high titres supported the positive findings in all instances. Sputum, when available, is clearly the best sample type for young adults with pneumonia.

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2005-03-01
2024-03-29
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References

  1. Brunner H. 1991; Adherence and pathogenicity of Mycoplasma pneumoniae : a review. In Microbial Surface Components and Toxins in Relation to Pathogenesis pp 81–89 Edited by Ron E., Rottem S. New York: Plenum;
    [Google Scholar]
  2. Casey H. L. 1965; Standardized diagnostic complement fixation method and adaptation to micro technique. Public Health Monogr 74:1–34
    [Google Scholar]
  3. Clyde W. A. Jr 1993; Clinical overview of typical Mycoplasma pneumoniae infections. Clin Infect Dis 17 Suppl 1:S32–S36
    [Google Scholar]
  4. Collier A. M., Clyde W. A. Jr 1974; Appearance of Mycoplasma pneumoniae in lungs of experimentally infected hamsters and sputum from patients with natural disease. Am Rev Respir Dis 110:765–773
    [Google Scholar]
  5. Fahle G. A., Fischer S. H. 2000; Comparison of six commercial DNA extraction kits for recovery of cytomegalovirus DNA from spiked human specimens. J Clin Microbiol 38:3860–3863
    [Google Scholar]
  6. Feikin D. R., Moroney J. F., Talkington D. F., Thacker W. L., Code J. E., Schwartz L. A., Erdman D. D., Butler J. C., Cetron M. S. 1999; An outbreak of acute respiratory disease caused by Mycoplasma pneumoniae and adenovirus at a federal service training academy: new implications from an old scenario. Clin Infect Dis 29:1545–1550
    [Google Scholar]
  7. Foy H. M. 1993; Infections caused by Mycoplasma pneumoniae and possible carrier state in different populations of patients. Clin Infect Dis 17 Suppl 1:S37–S46
    [Google Scholar]
  8. Foy H. M., Kenny G. E., Sefi R., Ochs H. D., Allan I. D. 1977; Second attacks of pneumonia due to Mycoplasma pneumoniae . J Infect Dis 135:673–677 [CrossRef]
    [Google Scholar]
  9. Heiskanen-Kosma T., Korppi M., Jokinen C. & 10 other authors; 1998; Etiology of childhood pneumonia: serologic results of a prospective, population-based study. Pediatr Infect Dis J 17:986–991 [CrossRef]
    [Google Scholar]
  10. Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B. C., Herrmann R. 1996; Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae . Nucleic Acids Res 24:4420–4449 [CrossRef]
    [Google Scholar]
  11. Jokinen C., Heiskanen L., Juvonen H. & 11 other authors; 2001; Microbial etiology of community-acquired pneumonia in the adult population of 4 municipalities in eastern Finland. Clin Infect Dis 32:1141–1154 [CrossRef]
    [Google Scholar]
  12. Kenny G. E., Kaiser G. G., Cooney M. K., Foy H. M. 1990; Diagnosis of Mycoplasma pneumoniae pneumonia: sensitivities and specificities of serology with lipid antigen and isolation of the organism on soy peptone medium for identification of infections. J Clin Microbiol 28:2087–2093
    [Google Scholar]
  13. Kleemola S. R., Karjalainen J. E., Räty R. K. 1990; Rapid diagnosis of Mycoplasma pneumoniae infection: clinical evaluation of a commercial probe test. J Infect Dis 162:70–75 [CrossRef]
    [Google Scholar]
  14. Kleemola M., Räty R., Karjalainen J., Schuy W., Gerstenecker B., Jacobs E. 1993; Evaluation of an antigen-capture enzyme immunoassay for rapid diagnosis of Mycoplasma pneumoniae infection. Eur J Clin Microbiol Infect Dis 12:872–875 [CrossRef]
    [Google Scholar]
  15. Nadal D., Bossart W., Zucol F., Steiner F., Berger C., Lips U., Altwegg M. 2001; Community-acquired pneumonia in children due to Mycoplasma pneumoniae : diagnostic performance of seminested 16S rDNA-PCR. Diagn Microbiol Infect Dis 39:15–19 [CrossRef]
    [Google Scholar]
  16. Petitjean J., Vabret A., Gouarin S., Freymuth F. 2002; Evaluation of four commercial immunoglobulin G (IgG)- and IgM-specific enzyme immunoassays for diagnosis of Mycoplasma pneumoniae infections. J Clin Microbiol 40:165–171 [CrossRef]
    [Google Scholar]
  17. Räty R., Kleemola M., Melen K., Stenvik M., Julkunen I. 1999; Efficacy of PCR and other diagnostic methods for the detection of respiratory adenoviral infections. J Med Virol 59:66–72 [CrossRef]
    [Google Scholar]
  18. Reznikov M., Blackmore T., Finlay-Jones J., Gordon D. 1995; Comparison of nasopharyngeal aspirates and throat swab specimens in a polymerase chain reaction-based test for Mycoplasma pneumoniae . Eur J Clin Microbiol Infect Dis 14:58–61 [CrossRef]
    [Google Scholar]
  19. Tjhie J. H., Van Kuppeveld F. J., Roosendaal R., Melchers W. J., Gordijn R., Maclaren D. M., Walboomers J. M., Meijer C. J., Van Den Brule A. J. 1994; Direct PCR enables detection of Mycoplasma pneumoniae in patients with respiratory tract infections. J Clin Microbiol 32:11–16
    [Google Scholar]
  20. Zhou X., Qin G. 2003; A new confidence interval for the difference between two binomial proportions of paired data. In UW Biostatistics Working Paper Series working paper 205 http://www.bepress.com/uwbiostat/paper205
    [Google Scholar]
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