1887

Abstract

Interleukin 8 (IL8) is usually produced in both epithelial and monocytic cells during bacterial infections, causing inflammation. induces production of IL8 from gastric epithelial cells via its pathogenicity island (cag PAI) system, LPS and outer-membrane protein. In some bacteria, heat-shock protein 60 (HSP60) also elicits a strong pro-inflammatory response in cells of the innate immune system. Three recombinant HSP60 (rHSP60) proteins of different sizes were produced and one of these was used to raise two monoclonal antibodies (2E7 and 7B5). IL8 production was found to be induced in cultured monocytic cells treated with cells or rHSP60 proteins, as measured by ELISA, and the amount of IL8 produced was dose-dependent. Pre-incubation of cells or rHSP60 preparations with the antibody 2E7 significantly inhibited IL8 production from monocytic cells. These results indicated that HSP60 is closely associated with IL8 production in monocytic cells.

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2005-03-01
2019-11-22
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References

  1. Bhattacharyya, A., Pathak, S., Datta, S., Chattopadhyay, S., Basu, J. & Kundu, M. ( 2002;). Mitogen-activated protein kinases and nuclear factor-κB regulate Helicobacter pylori-mediated interleukin-8 release from macrophages. Biochem J 368, 121–129.[CrossRef]
    [Google Scholar]
  2. Birkholz, S., Knipp, U., Nietzki, C., Adamek, R. J. & Opferkuch, W. ( 1993;). Immunological activity of lipopolysaccharide of Helicobacter pylori on human peripheral mononuclear blood cells in comparison to lipopolysaccharides of other intestinal bacteria. FEMS Immunol Med Microbiol 6, 317–324.[CrossRef]
    [Google Scholar]
  3. Blaser, M. J. ( 1990;). Helicobacter pylori and the pathogenesis of gastroduodenal inflammation. J Infect Dis 161, 626–633.[CrossRef]
    [Google Scholar]
  4. Bulut, Y., Faure, E., Thomas, L., Karahashi, H., Michelsen, K. S., Equils, O., Morrison, S. G., Morrison, R. P. & Arditi, M. ( 2002;). Chlamydial heat shock protein 60 activates macrophages and endothelial cells through Toll-like receptor 4 and MD2 in a MyD88-dependent pathway. J Immunol 168, 1435–1440.[CrossRef]
    [Google Scholar]
  5. Crabtree, J. E., Covacci, A., Farmery, S. M., Xiang, Z., Tompkins, D. S., Perry, S., Lindley, I. J. & Rappuoli, R. ( 1995;). Helicobacter pylori induced interleukin-8 expression in gastric epithelial cells is associated with CagA positive phenotype. J Clin Pathol 48, 41–45.[CrossRef]
    [Google Scholar]
  6. Crabtree, J. E., Kersulyte, D., Li, S. D., Lindley, I. J. & Berg, D. E. ( 1999;). Modulation of Helicobacter pylori induced interleukin-8 synthesis in gastric epithelial cells mediated by cag PAI encoded VirD4 homologue. J Clin Pathol 52, 653–657.[CrossRef]
    [Google Scholar]
  7. Cunningham, M. D., Shapiro, R. A., Seachord, C., Ratcliffe, K., Cassiano, L. & Darveau, R. P. ( 2000;). CD14 employs hydrophilic regions to ‘‘capture’’ lipopolysaccharides. J Immunol 164, 3255–3263.[CrossRef]
    [Google Scholar]
  8. Curry, J. L., Qin, J. Z., Bonish, B., Carrick, R., Bacon, P., Panella, J., Robinson, J. & Nickoloff, B. J. ( 2003;). Innate immune-related receptors in normal and psoriatic skin. Arch Pathol Lab Med 127, 178–186.
    [Google Scholar]
  9. D'Elios, M. M., Manghetti, M., Almerigogna, F. & 7 other authors ( 1997;). Different cytokine profile and antigen-specificity repertoire in Helicobacter pylori-specific T cell clones from the antrum of chronic gastritis patients with or without peptic ulcer. Eur J Immunol 27, 1751–1755.[CrossRef]
    [Google Scholar]
  10. Di Tommaso, A., Xiang, Z., Bugnoli, M., Pileri, P., Figura, N., Bayeli, P. F., Rappuoli, R., Abrignani, S. & De Magistris, M. T. ( 1995;). Helicobacter pylori-specific CD4+ T-cell clones from peripheral blood and gastric biopsies. Infect Immun 63, 1102–1106.
    [Google Scholar]
  11. Ernst, P. B. & Gold, B. D. ( 2000;). The disease spectrum of Helicobacter pylori: the immunopathogenesis of gastroduodenal ulcer and gastric cancer. Annu Rev Microbiol 54, 615–640.[CrossRef]
    [Google Scholar]
  12. Flohe, S. B., Bruggemann, J., Lendemans, S., Nikulina, M., Meierhoff, G., Flohe, S. & Kolb, H. ( 2003;). Human heat shock protein 60 induces maturation of dendritic cells versus a Th1-promoting phenotype. J Immunol 170, 2340–2348.[CrossRef]
    [Google Scholar]
  13. Friedland, J. S., Shattock, R., Remick, D. G. & Griffin, G. E. ( 1993;). Mycobacterial 65-kD heat shock protein induces release of proinflammatory cytokines from human monocytic cells. Clin Exp Immunol 91, 58–62.
    [Google Scholar]
  14. Gao, B. & Tsan, M. F. ( 2003;). Recombinant human heat shock protein 60 does not induce the release of tumor necrosis factor alpha from murine macrophages. J Biol Chem 278, 22523–22529.[CrossRef]
    [Google Scholar]
  15. Gobert, A. P., Bambou, J. C., Werts, C., Balloy, V., Chignard, M., Moran, A. P. & Ferrero, R. L. ( 2004;). Helicobacter pylori heat shock protein 60 mediates interleukin-6 production by macrophages via a toll-like receptor (TLR)-2-, TLR-4-, and myeloid differentiation factor 88-independent mechanism. J Biol Chem 279, 245–250.[CrossRef]
    [Google Scholar]
  16. Hayashi, S., Sugiyama, T., Yokota, K., Isogai, H., Isogai, E., Oguma, K., Asaka, M., Fujii, N. & Hirai, Y. ( 1998;). Analysis of immunoglobulin A antibodies to Helicobacter pylori in serum and gastric juice in relation to mucosal inflammation. Clin Diagn Lab Immunol 5, 617–621.
    [Google Scholar]
  17. Hinode, D., Yoshioka, M., Tanabe, S., Miki, O., Masuda, K. & Nakamura, R. ( 1998;). The GroEL-like protein from Campylobacter rectus: immunological characterization and interleukin-6 and -8 induction in human gingival fibroblast. FEMS Microbiol Lett 167, 1–6.[CrossRef]
    [Google Scholar]
  18. Huesca, M., Borgia, S., Hoffman, P. & Lingwood, C. A. ( 1996;). Acidic pH changes receptor binding specificity of Helicobacter pylori: a binary adhesion model in which surface heat shock (stress) proteins mediate sulfatide recognition in gastric colonization. Infect Immun 64, 2643–2648.
    [Google Scholar]
  19. Ishii, E., Yokota, K., Sugiyama, T. & 7 other authors ( 2001;). Immunoglobulin G1 antibody response to Helicobacter pylori heat shock protein 60 is closely associated with low-grade gastric mucosa-associated lymphoid tissue lymphoma. Clin Diagn Lab Immunol 8, 1056–1059.
    [Google Scholar]
  20. Kawahara, Y., Yokota, K., Mizuno, M. & 7 other authors ( 1999;). Antibodies to human gastric epithelial cells and heat shock protein 60 in Helicobacter pylori positive mucosa associated lymphoid tissue lymphoma. Gut 45, 20–23.[CrossRef]
    [Google Scholar]
  21. Kobayashi, K., Yokota, K., Yoshino, T., Kawahara, Y., Dey, A., Hirai, Y., Oguma, K. & Akagi, T. ( 1998;). Detection of Helicobacter pylori associated antigen and heat shock protein 60 on follicular dendritic cells in the germinal centres of low grade B cell lymphoma of gastric mucosa associated lymphoid tissue (MALT). J Clin Pathol 51, 396–398.[CrossRef]
    [Google Scholar]
  22. Lee, S. K., Stack, A., Katzowitsch, E., Aizawa, S. I., Suerbaum, S. & Josenhans, C. ( 2003;). Helicobacter pylori flagellins have very low intrinsic activity to stimulate human gastric epithelial cells via TLR5. Microbes Infect 5, 1345–1356.[CrossRef]
    [Google Scholar]
  23. MacDonald, A. J., Duffy, M., Brady, M. T., McKiernan, S., Hall, W., Hegarty, J., Curry, M. & Mills, K. H. ( 2002;). CD4 T helper type 1 and regulatory T cells induced against the same epitopes on the core protein in hepatitis C virus-infected persons. J Infect Dis 185, 720–727.[CrossRef]
    [Google Scholar]
  24. Moran, A. P., Helander, I. M. & Kosunen, T. U. ( 1992;). Compositional analysis of Helicobacter pylori rough-form lipopolysaccharides. J Bacteriol 174, 1370–1377.
    [Google Scholar]
  25. Oguma, K., Murayama, S., Syuto, B., Iida, H. & Kubo, S. ( 1984;). Analysis of antigenicity of Clostridium botulinum type C1 and D toxins by polyclonal and monoclonal antibodies. Infect Immun 43, 584–588.
    [Google Scholar]
  26. Parsonnet, J., Friedman, G. D., Vandersteen, D. P., Chang, Y., Vogelman, J. H., Orentreich, N. & Sibley, R. K. ( 1991;). Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med 325, 1127–1131.[CrossRef]
    [Google Scholar]
  27. Parsonnet, J., Hansen, S., Rodriguez, L., Gelb, A. B., Warnke, R. A., Jellum, E., Orentreich, N., Vogelman, J. H. & Friedman, G. D. ( 1994;). Helicobacter pylori infection and gastric lymphoma. N Engl J Med 330, 1267–1271.[CrossRef]
    [Google Scholar]
  28. Sharma, S. A., Miller, G. G., Peek, R. A., Jr, Perez-Perez, G. & Blaser, M. J. ( 1997;). T-cell, antibody, and cytokine responses to homologs of the 60-kilodalton heat shock protein in Helicobacter pylori infection. Clin Diagn Lab Immunol 4, 440–446.
    [Google Scholar]
  29. Sherry, B. & Cerami, A. ( 1991;). Small cytokine superfamily. Curr Opin Immunol 3, 56–60.[CrossRef]
    [Google Scholar]
  30. Su, B., Ceponis, P. J., Lebel, S., Huynh, H. & Sherman, P. M. ( 2003;). Helicobacter pylori activates Toll-like receptor 4 expression in gastrointestinal epithelial cells. Infect Immun 71, 3496–3502.[CrossRef]
    [Google Scholar]
  31. Takenaka, R., Yokota, K., Ayada, K. & 8 other authors ( 2004;). Helicobacter pylori-heat shock protein 60 induces inflammatory responses through the Toll-like receptor-triggered pathway in cultured human gastric epithelial cells. Microbiology 150, 3913–3922.[CrossRef]
    [Google Scholar]
  32. Wang, Y., Kelly, C. G., Singh, M., McGowan, E. G., Carrara, A. S., Bergmeier, L. A. & Lehner, T. ( 2002;). Stimulation of Th1-polarizing cytokines, C-C chemokines, maturation of dendritic cells, and adjuvant function by the peptide binding fragment of heat shock protein 70. J Immunol 169, 2422–2429.[CrossRef]
    [Google Scholar]
  33. Wotherspoon, A. C. ( 1998;). Gastric lymphoma of mucosa-associated lymphoid tissue and Helicobacter pylori. Annu Rev Med 49, 289–299.[CrossRef]
    [Google Scholar]
  34. Yamaguchi, H., Osaki, T., Kurihara, N., Kitajima, M., Kai, M., Takahashi, M., Taguchi, H. & Kamiya, S. ( 1999;). Induction of secretion of interleukin-8 from human gastric epithelial cells by heat-shock protein 60 homologue of Helicobacter pylori. J Med Microbiol 48, 927–933.[CrossRef]
    [Google Scholar]
  35. Yamaoka, Y., Kwon, D. H. & Graham, D. Y. ( 2000;). A M r 34,000 proinflammatory outer membrane protein (oipA) of Helicobacter pylori. Proc Natl Acad Sci U S A 97, 7533–7538.[CrossRef]
    [Google Scholar]
  36. Yamasaki, R., Yokota, K., Okada, H. & 7 other authors ( 2004;). Immune response in Helicobacter pylori-induced low-grade gastric-mucosa-associated lymphoid tissue (MALT) lymphoma. J Med Microbiol 53, 21–29.[CrossRef]
    [Google Scholar]
  37. Yunoki, N., Yokota, K., Mizuno, M. & 7 other authors ( 2000;). Antibody to heat shock protein can be used for early serological monitoring of Helicobacter pylori eradication treatment. Clin Diagn Lab Immunol 7, 574–577.
    [Google Scholar]
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