1887

Abstract

causes severe sepsis in humans. There are several reports about the relationship between host immunity and bacterial growth in infection. However, the effect on leukocytes of infection has not been elucidated. A murine model of infection was used to investigate its effects on leukocytes in this study. Bacteria were recovered from the blood of mice 3 h after subcutaneous injection in the right lower flank. They were detected in 87.5 % ( = 7/8) of mice at 6 h, but this value decreased to 12.5 % ( = 1/8) at 12 h. In contrast, the number of lymphocytes in peripheral blood had already started to decrease at 3 h, and reached a minimum at 6–9 h post-inoculation. Typical DNA laddering, a hallmark of apoptosis, was also detected in thymocytes and splenocytes at 6 and 9 h, and showed a tendency to disappear by 12 h. Although the number of lymphocytes decreased in the model, the numbers of neutrophils did not. These results suggested that has selective cytotoxicity for lymphocytes in peripheral blood , and the lymphocyte depletion was probably associated with apoptosis .

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2005-01-01
2019-11-19
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References

  1. Erridge, C., Pridmore, A., Eley, A., Stewart, J. & Poxton, I. R. ( 2004;). Lipopolysaccharides of Bacteroides fragilis, Chlamydia trachomatis and Pseudomonas aeruginosa signal via Toll-like receptor 2. J Med Microbiol 53, 735–740.[CrossRef]
    [Google Scholar]
  2. Fan, J. J., Shao, C. P., Ho, Y. C., Yu, C. K. & Hor, L. I. ( 2001;). Isolation and characterization of a Vibrio vulnificus mutant deficient in both extracellular metalloprotease and cytolysin. Infect Immun 69, 5943–5948.[CrossRef]
    [Google Scholar]
  3. Hlady, W. G. & Klontz, K. C. ( 1996;). The epidemiology of Vibrio infections in Florida, 1981–1993. J Infect Dis 173, 1176–1183.[CrossRef]
    [Google Scholar]
  4. Hotchkiss, R. S., Chang, K. C., Swanson, P. E. & 11 other authors ( 2000;). Caspase inhibitors improve survival in sepsis: a critical role of the lymphocyte. Nat Immunol 1, 496–501.[CrossRef]
    [Google Scholar]
  5. Hotchkiss, R. S., Tinsley, K. W., Swanson, P. E. & 8 other authors ( 2001;). Sepsis-induced apoptosis causes progressive profound depletion of B and CD4+ T lymphocytes in humans. J Immunol 166, 6952–6963.[CrossRef]
    [Google Scholar]
  6. Johnson, D. E., Calia, F. M., Musher, D. M. & Goree, A. ( 1984;). Resistance of Vibrio vulnificus to serum bactericidal and opsonizing factors: relation to virulence in suckling mice and humans. J Infect Dis 150, 413–418.[CrossRef]
    [Google Scholar]
  7. Kashimoto, T., Ueno, S., Hanajima, M., Hayashi, H., Akeda, Y., Miyoshi, S., Hongo, T., Honda, T. & Susa, N. ( 2003;). Vibrio vulnificus induces macrophage apoptosis in vitro and in vivo. Infect Immun 71, 533–535.[CrossRef]
    [Google Scholar]
  8. Kim, J. S., Chae, M. R., Chang, K., Park, K. H., Rho, H. W., Park, B. H., Park, J. W. & Kim, H. R. ( 1998;). Cytotoxicity of Vibrio vulnificus cytolysin on rat peritoneal mast cells. Microbiol Immunol 42, 837–843.[CrossRef]
    [Google Scholar]
  9. Koenig, K. L., Mueller, J. & Rose, T. ( 1991;). Vibrio vulnificus.Hazard on the half shell. West J Med 155, 400–403.
    [Google Scholar]
  10. Kreger, A., DeChatelet, L. & Shirley, P. ( 1981;). Interaction of Vibrio vulnificus with human polymorphonuclear leukocytes: association of virulence with resistance to phagocytosis. J Infect Dis 144, 244–248.[CrossRef]
    [Google Scholar]
  11. Kwon, K. B., Yang, J. Y., Ryu, D. G., Rho, H. W., Kim, J. S., Park, J. W., Kim, H. R. & Park, B. H. ( 2001;). Vibrio vulnificus cytolysin induces superoxide anion-initiated apoptotic signaling pathway in human ECV304 cells. J Biol Chem 276, 47518–47523.[CrossRef]
    [Google Scholar]
  12. Linkous, D. A. & Oliver, J. D. ( 1999;). Pathogenesis of Vibrio vulnificus. FEMS Microbiol Lett 174, 207–214.[CrossRef]
    [Google Scholar]
  13. Miyoshi, S., Nakazawa, H., Kawata, K., Tomochika, K., Tobe, K. & Shinoda, S. ( 1998;). Characterization of the hemorrhagic reaction caused by Vibrio vulnificus metalloprotease, a member of the thermolysin family. Infect Immun 66, 4851–4855.
    [Google Scholar]
  14. Morris, J. G., Jr, Wright, A. C., Simpson, L. M., Wood, P. K., Johnson, D. E. & Oliver, J. D. ( 1987;). Virulence of Vibrio vulnificus: association with utilization of transferring-bound iron, and lack of correlation with levels of cytotoxin or protease production. FEMS Microbiol Lett 40, 55–59.
    [Google Scholar]
  15. Poltorak, A., He, X., Smirnova, I. & 11 other authors ( 1998;). Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science 282, 2085–2088.[CrossRef]
    [Google Scholar]
  16. Starks, A. M., Schoeb, T. R., Tamplin, M. L., Parveen, S., Doyle, T. J., Bomeisl, P. E., Escudero, G. M. & Gulig, P. A. ( 2000;). Pathogenesis of infection by clinical and environmental strains of Vibrio vulnificus in iron-dextran-treated mice. Infect Immun 68, 5785–5793.[CrossRef]
    [Google Scholar]
  17. Stelma, G. N., Jr, Reyes, A. L., Peeler, J. T., Johnson, C. H. & Spaulding, P. L. ( 1992;). Virulence characteristics of clinical and environmental isolates of Vibrio vulnificus. Appl Environ Microbiol 58, 2776–2782.
    [Google Scholar]
  18. Strom, M. S. & Paranjpye, R. N. ( 2000;). Epidemiology and pathogenesis of Vibrio vulnificus. Microbes Infect 2, 177–188.[CrossRef]
    [Google Scholar]
  19. Szondy, Z., Reichert, U., Bernardon, J. M., Michel, S., Toth, R., Karaszi, E. & Fesus, L. ( 1998;). Inhibition of activation-induced apoptosis of thymocytes by all-trans- and 9-cis-retinoic acid is mediated via retinoic acid receptor alpha. Biochem J 331, 767–774.
    [Google Scholar]
  20. Tamplin, M. L., Specter, S., Rodrick, G. E. & Friedman, H. ( 1985;). Vibrio vulnificus resists phagocytosis in the absence of serum opsonins. Infect Immun 49, 715–718.
    [Google Scholar]
  21. Wright, A. C., Simpson, L. M. & Oliver, J. D. ( 1981;). Role of iron in the pathogenesis of Vibrio vulnificus infections. Infect Immun 34, 503–507.
    [Google Scholar]
  22. Yoshida, S., Ogawa, M. & Mizuguchi, Y. ( 1985;). Relation of capsular materials and colony opacity to virulence of Vibrio vulnificus. Infect Immun 47, 446–451.
    [Google Scholar]
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