1887

Abstract

In this study the raising and development of the immune response to infection in 45 Italian patients suffering from culture-confirmed Lyme borreliosis erythema migrans was investigated. A total of 95 serially collected serum samples were tested by using three different commercial ELISAs: recomWell Borrelia (Mikrogen), Enzygnost Borreliosis (DADE Behring) and Quick ELISA C6 Borrelia (Immunetics). The sensitivities of the ELISAs were as follows: Enzygnost Borreliosis IgM, 70.5 %; Quick ELISA C6 Borrelia, 62.1 %; recomWell Borrelia IgM, 55.7 %; recomWell Borrelia IgG, 57.9 %; and Enzygnost Borreliosis IgG, 36.8 %. In order to compare the specificity values of the three ELISAs, a panel of sera obtained from blood donors (210 samples coming from a non-endemic area and 24 samples from an endemic area) was tested, as well as sera from patients suffering from some of the most common biological conditions that could result in false-positive reactivity in Lyme disease serology ( = 40). RecomWell Borrelia IgG and recomWell Borrelia IgM were the most specific (97.1 % and 98.9 %, respectively), followed by Quick ELISA C6 Borrelia (96.7 %). Enzygnost Borreliosis IgG and IgM achieved 90.1 % and 92.3 % specificity, respectively. Sera that gave discrepant results when tested by the three ELISAs were further analysed by Western blotting.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.45853-0
2005-04-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jmm/54/4/JM540408.html?itemId=/content/journal/jmm/10.1099/jmm.0.45853-0&mimeType=html&fmt=ahah

References

  1. Aguero-Rosenfeld, M. E., Nowakowski, J., Bittker, S., Cooper, D., Nadelman, R. B. & Wormser, G. P. ( 1996;). Evolution of the serologic response to Borrelia burgdorferi in treated patients with culture-confirmed erythema migrans. J Clin Microbiol 34, 1–9.
    [Google Scholar]
  2. Bacon, R. M., Biggerstaff, B. J., Schriefer, M., Gilmore, R. D., Philipp, M. T., Steere, A., Wormser, G. P., Marques, A. R. & Johnson, B. J. B. ( 2003;). Serodiagnosis of Lyme disease by kinetic enzyme-linked immunosorbent assay using recombinant VlsE1 or peptide antigens of Borrelia burgdorferi compared with two-tiered testing using whole-cell lysates. J Infect Dis 187, 1187–1199.[CrossRef]
    [Google Scholar]
  3. Baranton, G., Postic, D., Saint Girons, I., Boerlin, P., Piffaretti, J. C., Assous, M. & Grimont, P. A. D. ( 1992;). Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp.nov., and group VS461 associated with Lyme borreliosis. Int J Syst Bacteriol 42, 378–383.[CrossRef]
    [Google Scholar]
  4. Centers for Disease Control & Prevention ( 1995;). Recommendations for test performance and interpretation from the Second National Conference on Serological Diagnosis of Lyme Disease. Morb Mortal Wkly Rep 44, 590–591.
    [Google Scholar]
  5. Cevenini, R., Sambri, V., Massaria, F., Franchini, R., D'Antuono, A., Borda, G. & Negosanti, M. ( 1992;). Surface immunofluorescence assay for diagnosis of Lyme disease. J Clin Microbiol 30, 2456–2461.
    [Google Scholar]
  6. Ciceroni, L., Ciarrochi, S., Ciervo, A., Mondarini, V., Guzzo, F., Caruso, G., Murgia, R. & Cinco, M. ( 2001;). Isolation and characterization of Borrelia burgdorferi sensu lato strains in an area of Italy where Lyme borreliosis is endemic. J Clin Microbiol 39, 2254–2260.[CrossRef]
    [Google Scholar]
  7. Gern, L., Estrada-Pena, A., Frandsen, F. & 7 other authors ( 1998;). European reservoir hosts of Borrelia burgdorferi sensu lato. Zentbl Bakteriol 287, 196–204.[CrossRef]
    [Google Scholar]
  8. Hauser, U., Lehnert, G., Lobentanzer, R. & Wilske, B. ( 1997;). Interpretation criteria for standardized Western blots for three European species of Borrelia burgdorferi sensu lato. J Clin Microbiol 35, 1433–1444.
    [Google Scholar]
  9. Hauser, U., Krahl, H., Peters, H., Fingerle, V. & Wilske, B. ( 1998;). Impact of strain heterogeneity on Lyme disease serology in Europe: comparison of enzyme-linked immunosorbent assays using different species of Borrelia burgdorferi sensu lato. J Clin Microbiol 36, 427–436.
    [Google Scholar]
  10. Hauser, U., Lehnert, G. & Wilske, B. ( 1999;). Validity of interpretation criteria for standardized Western blots (immunoblots) for serodiagnosis of Lyme borreliosis based on sera collected throughout Europe. J Clin Microbiol 37, 2241–2247.
    [Google Scholar]
  11. Heikkila, T., Seppala, I., Saxen, H., Panelius, J., Yrjanainen, H. & Lahdenne, P. ( 2002;). Species-specific serodiagnosis of Lyme arthritis and neuroborreliosis due to Borrelia burgdorferi sensu stricto, B.afzelii, and B. garinii by using decorin binding protein A. J Clin Microbiol 40, 453–460.[CrossRef]
    [Google Scholar]
  12. Laemmli, U. K. ( 1970;). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef]
    [Google Scholar]
  13. Lawrenz, M. B., Hardham, J. M., Owens, R. T., Nowakowski, J., Steere, A. C., Wormser, G. P. & Norris, S. J. ( 1999;). Human antibody responses to VlsE antigenic variation protein of Borrelia burgdorferi. J Clin Microbiol 37, 3997–4004.
    [Google Scholar]
  14. Liang, F. T., Steere, A. C., Marques, A. R., Johnson, B. J. B., Miller, J. N. & Philipp, M. T. ( 1999;). Sensitive and specific serodiagnosis of Lyme disease by enzyme-linked immunosorbent assay with a peptide based on an immunodominant conserved region of Borrelia burgdorferi VlsE. J Clin Microbiol 37, 3990–3996.
    [Google Scholar]
  15. Magnarelli, L. A., Miller, J. N., Anderson, J. F. & Riviere, G. F. ( 1990;). Cross-reactivity of nonspecific treponemal antibody in serologic tests for Lyme disease. J Clin Microbiol 28, 1276–1279.
    [Google Scholar]
  16. Magnarelli, L. A., Anderson, J. F., Johnson, R. C., Nadelman, R. B. & Wormser, G. P. ( 1994;). Comparison of different strains of Borrelia burgdorferi sensu lato used as antigens in enzyme-linked immunosorbent assays. J Clin Microbiol 32, 1154–1158.
    [Google Scholar]
  17. Magnarelli, L. A., Ijdo, J. W., Padula, S. J., Flavell, R. A. & Fikrig, E. ( 2000;). Serologic diagnosis of Lyme borreliosis by using enzyme-linked immunosorbent assays with recombinant antigens. J Clin Microbiol 38, 1735–1739.
    [Google Scholar]
  18. Magnarelli, L. A., Lawrenz, M., Norris, S. J. & Fikrig, E. ( 2002;). Comparative reactivity of human sera to recombinant VlsE and other Borrelia burgdorferi antigens in class-specific enzyme-linked immunosorbent assays for Lyme borreliosis. J Med Microbiol 51, 649–655.
    [Google Scholar]
  19. Marangoni, A., Sambri, V., Cimmino, C., Caruso, G., Mondardini, V. & Cevenini, R. ( 1999;). Evaluation of the immune response in culture-confirmed Lyme borreliosis erythema migrans patients. Zentbl Bakteriol 289, 736–739.[CrossRef]
    [Google Scholar]
  20. Marconi, R. T. & Garon, C. F. ( 1992;). Development of chain reaction primers sets for diagnosis of Lyme disease and for species-specific identification of Lyme disease isolates by 16S rRNA signature nucleotide analysis. J Clin Microbiol 30, 2830–2834.
    [Google Scholar]
  21. Nadelman, R. B. & Wormser, G. P. ( 1998;). Lyme borreliosis. Lancet 352, 557–565.[CrossRef]
    [Google Scholar]
  22. Nadelman, R. B., Nowakowski, J., Forseter, G., Goldberg, N. S., Bittker, S., Cooper, D., Aguero-Rosenfeld, M. & Wormser, G. P. ( 1996;). The clinical spectrum of early Lyme borreliosis in patients with culture-confirmed erythema migrans. Am J Med 100, 502–508.[CrossRef]
    [Google Scholar]
  23. O'Connell, S., Granström, M., Gray, J. S. & Stanek, G. ( 1998;). Epidemiology of European Lyme borreliosis. Zentbl Bakteriol 287, 229–240.[CrossRef]
    [Google Scholar]
  24. Peltomaa, M., McHugh, G. & Steere, A. C. ( 2003;). Persistence of the antibody response to the VlsE sixth invariant region (IR6) peptide of Borrelia burgdorferi after successful antibiotic treatment of Lyme disease. J Infect Dis 187, 1178–1186.[CrossRef]
    [Google Scholar]
  25. Picken, R. N. ( 1992;). Polymerase chain reaction primers and probes derived from flagellin gene sequences for specific detection of the agents of Lyme disease and North American relapsing fever. J Clin Microbiol 30, 99–114.
    [Google Scholar]
  26. Robertson, J., Guy, E., Andrews, N. & 9 other authors ( 2000;). A European multicenter study of immunoblotting in serodiagnosis of lyme borreliosis. J Clin Microbiol 38, 2097–2102.
    [Google Scholar]
  27. Sambri, V., Marangoni, A., Olmo, A., Storni, E., Montagnani, M., Fabbi, M. & Cevenini, R. ( 1999;). Specific antibodies reactive with the 22-kilodalton major outer surface protein of Borrelia anserina Ni-NL protect chicks from infection. Infect Immun 67, 2633–2637.
    [Google Scholar]
  28. Sambri, V., Marangoni, A., Eyer, C., Reichhuber, C., Soutschek, E., Negosanti, M., D'Antuono, A. & Cevenini, R. ( 2001;). Western immunoblotting with five Treponema pallidum recombinant antigens for serological diagnosis of syphilis. Clin Diagn Lab Immunol 8, 534–539.
    [Google Scholar]
  29. Sambri, V., Marangoni, A., Giacani, L., Gennaro, R., Murgia, R., Cevenini, R. & Cinco, M. ( 2002;). Comparative in vitro activity of five cathelicidin-derived synthetic peptides against Leptospira, Borrelia and Treponema pallidum. J Antimicrob Chemother 50, 895–902.[CrossRef]
    [Google Scholar]
  30. Stanek, G. & Strle, F. ( 2003;). Lyme borreliosis. Lancet 362, 1639–1647.[CrossRef]
    [Google Scholar]
  31. Steere, A. C. ( 1994;). Lyme disease: a growing threat to urban populations. Proc Natl Acad Sci U S A 91, 2378–2383.[CrossRef]
    [Google Scholar]
  32. Strle, F., Nelson, J. A., Ruzic-Sabljic, E. & 7 other authors ( 1996;). European Lyme borreliosis: 231 culture-confirmed cases involving patients with erythema migrans. Clin Infect Dis 23, 61–65.[CrossRef]
    [Google Scholar]
  33. Towbin, H., Staehelin, T. & Gordon, J. ( 1979;). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 76, 4350–4354.[CrossRef]
    [Google Scholar]
  34. Wang, G., van Dam, A. P., Schwartz, I. & Dankert, J. ( 1999;). Molecular typing of Borrelia burgdorferi sensu lato: taxonomic, epidemiological, and clinical implications. Clin Microbiol Rev 12, 633–653.
    [Google Scholar]
  35. Wilske, B., Busch, U., Eiffert, H., Fingerle, V., Pfister, H. W., Rössler, D. & Preac-Mursic, V. ( 1996;). Diversity of OspA and OspC among cerebrospinal fluid isolates of Borrelia burgdorferi sensu lato from patients with neuroborreliosis in Germany. Med Microbiol Immunol 184, 195–201.[CrossRef]
    [Google Scholar]
  36. Wilske, B., Zöller, L., Brade, V., Eiffert, M., Göbel, U. B., Stanek, G. & Pfister, H. W. ( 2000;). MIQ 12 Lyme-Borreliose. In Qualitätsstandards in der Mikrobiologisch-Infektiologischen Diagnostik, pp. 1–59. Edited by H. Mauch & R. Lütticken. Munich, Germany: Urban & Fischer Verlag.
  37. Wormser, G. P., Carbonaro, C., Miller, S., Nowakowski, J., Nadelman, R. B., Sivak, S. & Aguero-Rosenfeld, M. E. ( 2000;). A limitation of 2-stage serological testing for Lyme disease: enzyme immunoassay and immunoblot assay are not independent tests. Clin Infect Dis 30, 545–548.[CrossRef]
    [Google Scholar]
  38. Zhang, J. R., Hardham, J. M., Barbour, A. G. & Norris, S. J. ( 1997;). Antigenic variation in Lyme disease borreliae by promiscuous recombination of VMP-like sequence cassettes. Cell 89, 275–285.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.45853-0
Loading
/content/journal/jmm/10.1099/jmm.0.45853-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error