1887

Abstract

Stool samples from seven human immunodeficiency virus (HIV)-negative and two HIV-positive children with asymptomatic infections were daily examined to quantify spore shedding using Gram-chromotrope staining under light microscopy. The spore shedding pattern and intensity in these children was variable. Mean spore concentrations in the stool samples from these children ranged from 2.4 × 10 to 1.2 × 10 spores per gram. Light microscopy could detect spores in stool specimens for 9–33 days, while PCR was able to detect in stool specimens for 3–40 days longer. This suggests that light microscopy may not detect low levels of spore shedding. Considering that the asymptomatic group are a potential source of infection, detection methods with a higher sensitivity should be used.

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2005-05-01
2019-11-14
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