1887

Abstract

is a halophilic species associated with acute diarrhoeal illness in humans. It has the potential to cause outbreaks and has an association with paediatric diarrhoea. In this study, 11 strains isolated from hospitalized patients with acute diarrhoea at the Infectious Diseases Hospital, Kolkata were extensively characterized. All the strains showed growth in peptone broth containing 7 % NaCl. The strains showed variable results in Voges–Proskauer test and to a vibriostatic agent. There was also variation in their antibiograms, and some of the strains were multidrug resistant. Among the 11 strains, two showed only a single band difference in their PFGE profile and the remaining strains showed nine different PFGE patterns. However, unlike PFGE, the strains exhibited close matches and clustering in their ribotype patterns. The haemolytic effect on sheep red blood cells varied with strains. Partial sequence analysis revealed that the haemolysin gene has 81 % homology with that of the El Tor haemolysin of . A striking finding was the capability of all the strains to evoke distinct cytotoxic and vacuolation effects on HeLa cells.

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2005-08-01
2019-11-22
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References

  1. Abrami, L., Fivaz, M., Glauser, P. E., Parton, R. G. & Van der Goot, F. G. ( 1998;). A pore-forming toxin interacts with a GPI-anchored protein and causes vacuolation of the endoplasmic reticulum. J Cell Biol 140, 525–540.[CrossRef]
    [Google Scholar]
  2. Albert, M. J., Hossain, M. A., Alam, K., Kabir, I., Neogi, P. K. B. & Tzipori, S. ( 1991;). A fatal case associated with shigellosis and Vibrio fluvialis bacteremia. Diagn Microbiol Infect Dis 14, 509–510.[CrossRef]
    [Google Scholar]
  3. Alm, R. A., Stroeher, U. H. & Manning, P. A. ( 1988;). Extracellular proteins of Vibrio cholerae: nucleotide sequence of the structural gene (hlyA) for the haemolysin of the haemolytic El Tor strain O17 and characterization of the hlyA mutation in the non-haemolytic classical 569B. Mol Microbiol 2, 481–488.[CrossRef]
    [Google Scholar]
  4. Baselski, V., Briggs, R. & Parker, C. ( 1977;). Intestinal fluid accumulation induced by oral challenge with Vibrio cholerae or cholera toxin in infant mice. Infect Immun 15, 704–712.
    [Google Scholar]
  5. Brenner, D. J., Hickman-Brenner, F. W., Lee, J. V. & 7 other authors, ( 1983;). Vibrio furnissii (formerly aerogenic biogroup of Vibrio fluvialis), a new species isolated from human feces and the environment. J Clin Microbiol 18, 816–824.
    [Google Scholar]
  6. Brosius, J., Ullrich, A., Raber, M. A., Garg, A., Dull, J. J., Gutell, R. R. & Noler, H. F. ( 1981;). Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA operon of Escherichia coli. Plasmid 6, 112–118.[CrossRef]
    [Google Scholar]
  7. Carroll, P. A., Tashima, K. T., Rogers, M. B., Dirita, V. J. & Calderwood, S. B. ( 1997;). Phase variation in tcpH modulates expression of the ToxR rugulon in Vibrio cholerae. Mol Microbiol 25, 1099–1111.[CrossRef]
    [Google Scholar]
  8. Cover, T. L. & Blaser, M. J. ( 1992;). Purification and characterization of the vacuolating toxin from Helicobacter pylori. J Biol Chem 267, 10570–10575.
    [Google Scholar]
  9. Cowan, S. T. ( 1979;). Manual for the Identification of Medical Bacteria, 2nd edn. Edited by S. T. Cowan & K. J. Steel. Cambridge: Cambridge University Press.
  10. De, S. N. & Chatterjee, D. N. ( 1953;). An experimental study on mechanism of action of Vibrio cholerae on the intestinal mucous membrane. J Pathol Bacteriol 66, 559–562.[CrossRef]
    [Google Scholar]
  11. Furniss, A. L., Lee, J. V. & Donovan, T. J. ( 1977;). Group F, a new Vibrio? Lancet ii, 565–566.
    [Google Scholar]
  12. Hertle, R., Hilger, M., Weingardt-Kocher, S. & Walev, I. ( 1999;). Cytotoxic action of Serratia marcescens hemolysin on human epithelial cells. Infect Immun 67, 817–825.
    [Google Scholar]
  13. Hickman-Brenner, F. W., Brenner, D. J., Steigerwalt, A. G., Schreiber, M., Holmberg, S. D., Baldy, L. M., Lewis, C. S., Pickens, N. M. & Farmer, J. J., III ( 1984;). Vibrio fluvialis and Vibrio furnissii isolated from a stool sample of one patient. J Clin Microbiol 20, 125–127.
    [Google Scholar]
  14. Hodge, T. W., Jr, Levy, C. S. & Smith, M. A. ( 1995;). Diarrhea associated with Vibrio fluvialis infection in a patient with AIDS. Clin Infect Dis 21, 237–238[CrossRef]
    [Google Scholar]
  15. Huq, M. I., Alam, A. K. M. J., Brenner, D. J. & Morris, G. K. ( 1980;). Isolation of Vibrio–like group, EF-6, from patients with diarrhea. J Clin Microbiol 11, 621–624.
    [Google Scholar]
  16. Huq, M. I., Aziz, K. M. S. & Colwell, R. R. ( 1985;). Enterotoxigenic properties of Vibrio fluvialis (group F Vibrio) isolated from clinical and environmental sources. J Diarrhoeal Dis Res 3, 96–99.
    [Google Scholar]
  17. Kaper, J. B. ( 1979;). Isolation, ecology and taxonomy of human pathogens in an estuary. PhD thesis, University of Maryland.
  18. Karaolis, D. K. R., Johnson, J. A., Bailey, C. C., Boedeker, E. C., Kaper, J. B. & Reeves, P. R. ( 1998;). A Vibrio cholerae pathogenicity island associated with epidemic and pandemic strains. Proc Natl Acad Sci U S A 95, 3134–3139.[CrossRef]
    [Google Scholar]
  19. Kay, B. A., Bopp, C. A. & Wells, J. G. ( 1994;). Isolation and identification of Vibrio cholerae O1 from fecal specimens. In Vibrio cholerae and Cholera: Molecular to Global Perspective, pp. 3–25. Edited by I. K. Wachsmuth, P. A. Blake & O. Olsvik. Washington, DC: ASM Press.
  20. Keasler, S. P. & Hall, R. H. ( 1993;). Detecting and biotyping Vibrio cholerae O1 with multiplex polymerase chain reaction. Lancet 341, 1661. 1661.[CrossRef]
    [Google Scholar]
  21. Kim, Y. B., Okuda, J., Matsumoto, C., Takahastu, N., Hashimoto, S. & Nishibuchi, M. ( 1999;). Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene. J Clin Microbiol 37, 1173–1177.
    [Google Scholar]
  22. Klontz, K. C., Cover, D. E., Hyman, F. N. & Mullen, R. C. ( 1994;). Fatal gastroenteritis due to Vibrio fluvialis and nonfatal bacteremia due to Vibrio mimicus: unusual Vibrio infections in two patients. Clin Infect Dis 19, 541–542.[CrossRef]
    [Google Scholar]
  23. Kobayashi, K. & Ohnaka, T. ( 1989;). Food poisoning due to newly recognized pathogens. Asian Med J 32, 1–12.
    [Google Scholar]
  24. Kothary, M. H., Lowman, H., McCardell, B. A. & Tall, B. D. ( 2003;). Purification and characterization of enterotoxigenic El Tor-like hemolysin produced by Vibrio fluvialis. Infect Immun 71, 3213–3220.[CrossRef]
    [Google Scholar]
  25. Kovach, M. E., Shaffer, M. D. & Peterson, K. M. ( 1996;). A putative integrase genes defines the distal end of a large cluster of ToxR-regulated colonization genes in Vibrio cholerae. Microbiology 142, 2165–2174.[CrossRef]
    [Google Scholar]
  26. Kurazono, H., Okuda, J., Takeda, Y., Nair, G. B., Albert, M. J., Sack, R. B., Chongsanguan, M. & Chaicupma, W. ( 1994;). Vibrio cholerae O139 Bengal isolated from India, Bangladesh and Thailand are clonal as determined by pulsed field gel electrophoresis. J Infect 29, 109–110.[CrossRef]
    [Google Scholar]
  27. Lee, J. V., Shread, P., Furniss, A. L. & Bryant, T. N. ( 1981;). Taxonomy and description of Vibrio fluvialis sp.nov. (synonym group F Vibrios, group EF-6). J Appl Bacteriol 50, 73–94.[CrossRef]
    [Google Scholar]
  28. Leunk, R. D., Johnson, P. T., David, B. C., Kraft, W. G. & Morgan, D. R. ( 1988;). Cytotoxin activity in broth-culture filtrates of Campylobacter pylori. J Med Microbiol 26, 93–99.[CrossRef]
    [Google Scholar]
  29. Levine, W. C. & Griffin, P. M. ( 1993;). Vibrio infections on the Gulf Coast: results of first year of regional surveillance.Gulf coast Vibrio working group. J Infect Dis 167, 479–483.
    [Google Scholar]
  30. Lockwood, D. E., Kreger, A. S. & Richardson, S. H. ( 1982;). Detection of toxins produced by Vibrio fluvialis. Infect Immun 35, 702–708.
    [Google Scholar]
  31. Manning, P. A., Brown, M. H. & Heuzenroeder, M. W. ( 1984;). Cloning of the structural gene (hly) for the haemolysin of Vibrio cholerae El Tor strain O17. Gene 31, 225–231.[CrossRef]
    [Google Scholar]
  32. McNicol, L. A., Kaper, J. B., Lockman, H. A., Remmers, E. F., Spira, W. M., Voll, M. J. & Colwell, R. R. ( 1980;). R-factor carriage in a group F Vibrio isolated from Bangladesh. Antimicrob Agents Chemother 17, 512–515.[CrossRef]
    [Google Scholar]
  33. Miller, V. L., Taylor, R. K. & Mekalanos, J. J. ( 1987;). Cholera toxin transcriptional activator ToxR is a transmembrane DNA binding protein. Cell 48, 271–279.[CrossRef]
    [Google Scholar]
  34. Mitra, R., Figueroa, P., Mukhopadhyay, A. K., Shimada, T., Takeda, Y., Berg, D. & Nair, G. B. ( 2000;). Cell vacuolation, a manifestation of the El Tor hemolysin of Vibrio cholerae. Infect Immun 68, 1928–1933.[CrossRef]
    [Google Scholar]
  35. Mitra, R. K., Nandy, R. K., Ramamurthy, T., Bhattacharya, S. K., Yamasaki, S., Shimada, T., Takeda, Y. & Nair, G. B. ( 2001;). Molecular characterisation of rough variants of Vibrio cholerae isolated from hospitalised patients with diarrhoea. J Med Microbiol 50, 268–276.
    [Google Scholar]
  36. Morris, J. G., Jr, & Black, R. E. ( 1985;). Cholera and other vibrios in the United States. N Engl J Med 312, 343–350.[CrossRef]
    [Google Scholar]
  37. Murray, M. G. & Thomson, W. F. ( 1980;). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8, 4321–4325.[CrossRef]
    [Google Scholar]
  38. Nair, G. B., Misra, S., Bhadra, R. K. & Pal, S. C. ( 1987;). Evaluation of the multitest medium for rapid presumptive identification of Vibrio cholerae from environmental sources. Appl Environ Microbiol 53, 1203–1205.
    [Google Scholar]
  39. Nandi, B., Nandy, R. K., Mukhopadhyay, S., Nair, G. B., Shimada, T. & Ghose, A. C. ( 2000;). Rapid method for species-specific identification of Vibrio cholerae using primers targeted to the gene of outer membrane protein OmpW. J Clin Microbiol 38, 4145–4151.
    [Google Scholar]
  40. NCCLS ( 2002;). Performance Standards for Antimicrobial Susceptibility Testing, 12th informational supplement. NCCLS document M100-S12. Wayne, PA: National Committee for Clinical Laboratory Standards.
  41. Nishibuchi, M. & Seidler, R. J. ( 1983;). Medium-dependent production of extracellular enterotoxins by non-O1 Vibrio cholerae, Vibrio mimicus and Vibrio fluvialis. Appl Environ Microbiol 45, 228–231.
    [Google Scholar]
  42. Ogawa, A., Kato, J. I., Watanabe, H., Nair, G. B. & Takeda, T. ( 1990;). Cloning and nucleotide sequence of a heat-stable enterotoxin gene from Vibrio cholerae non-O1 isolated from a patient with traveler's diarrhea. Infect Immun 58, 3325–3329.
    [Google Scholar]
  43. Patel, J. B., Leonard, D. G. B., Pan, X., Musser, J. M., Berman, R. E. & Nachamkin, I. ( 2000;). Sequenced-based identification of Mycobacterium species using the Microseq 500 16S rDNA bacterial identification system. J Clin Microbiol 38, 246–251.
    [Google Scholar]
  44. Ramamurthy, T., Pal, A., Pal, S. C. & Nair, G. B. ( 1992;). Taxonomical implications of the emergence of high frequency of occurrence of 2,4-diamino-6,7-diisopropylpteridine-resistant strains of Vibrio cholerae from clinical cases of cholera in Calcutta, India. J Clin Microbiol 30, 742–743.
    [Google Scholar]
  45. Ramamurthy, T., Bag, P. K., Pal, A. & 7 other authors ( 1993;). Virulence patterns of Vibrio cholerae non-O1 strains isolated from hospitalized patients with acute diarrhoea in Calcutta, India. J Med Microbiol 39, 310–317.[CrossRef]
    [Google Scholar]
  46. Sanyal, S. C., Agarwal, R. K., Annapurna, E. & Lee, J. V. ( 1980;). Enterotoxicity of group F vibrios. Jpn J Med Sci Biol 33, 217–222.[CrossRef]
    [Google Scholar]
  47. Seidler, R. J., Allen, D. A., Colwell, R. R., Joseph, S. W. & Daily, O. P. ( 1980;). Biochemical characteristics and virulence of environmental group F bacteria isolated in the United States. Appl Environ Microbiol 40, 715–720.
    [Google Scholar]
  48. Suthienkul, O., Ohashi, M., Goto, S., Sanyal, S. C. & Echeverria, P. ( 1985;). The enterotoxigenicity of Vibrio fluvialis isolated from aquatic sources in Thailand. J Diarrhoeal Dis Res 3, 14–19.
    [Google Scholar]
  49. Tacket, C. O., Hickman, F., Pierce, G. V. & Mendoza, L. F. ( 1982;). Diarrhoea associated with Vibrio fluvialis in the United States. J Clin Microbiol 16, 991–992.
    [Google Scholar]
  50. Tada, J., Ohashi, T., Nishimura, N., Shirasaki, Y., Ozaki, H., Fukushima, S., Takano, J., Nishibuchi, M. & Takeda, Y. ( 1992;). Detection of the thermostable direct hemolysin gene (tdh) and the thermostable direct haemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus by polymerase chain reaction. Mol Cell Probes 6, 477–487.[CrossRef]
    [Google Scholar]
  51. Thekdi, R., Lakhani, A. G., Vachha, S. M. & Chandrakapure, M. R. ( 1982;). Vibrio fluvialis (group F Vibrio) in Maharashtra. Indian J Med Res 76, 80–85.
    [Google Scholar]
  52. Thekdi, R. J., Lakhani, A. G., Rale, V. B. & Panse, M. V. ( 1990;). An outbreak of food poisoning suspected to be caused by Vibrio fluvialis. J Diarrhoeal Dis Res 8, 163–165.
    [Google Scholar]
  53. Wall, V. W., Kreger, A. S. & Richardson, S. H. ( 1984;). Production and partial characterization of a Vibrio fluvialis cytotoxin. Infect Immun 46, 773–777.
    [Google Scholar]
  54. WHO ( 1987;). Manual for Laboratory Identification of Acute Enteric Infections, CDD/83-3/Rev 1987.113. Geneva, Switzerland; World Health Organization.
  55. WHO ( 1995;). Assessment of the child with diarrhoea. In The Treatment of Diarrhoea: a Manual for Physicians and other Senior Health Workers, pp 6–7. Geneva, Switzerland: World Health Organization.
  56. Yoshii, Y., Nishino, H., Satake, K. & Umeyama, K. ( 1987;). Isolation of Vibrio fluvialis, an unusual pathogen in acute suppurative cholangitis. Am J Gastroenterol 82, 903–905.
    [Google Scholar]
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