1887

Abstract

The increasing incidence of associated disease, and the problems associated with its control, highlight the need for additional countermeasures. The attenuation of virulence through the blockade of bacterial cell-to-cell communication (quorum sensing) is one potential therapeutic target. Preliminary studies have shown that produces at least one potential signalling molecule. Through the molecule's ability to induce bioluminescence in a reporter strain, it has been shown to correspond to autoinducer 2 (AI-2). In keeping with this observation, a homologue of has been identified in the genome of . Adjacent to a potential transcriptional regulator and sensor kinase, and , have been located. RT-PCR has been used to confirm the genetic organization of the locus. While AI-2 production has not been blocked so far using antisense technology, AI-2 levels could be modulated by controlling expression of the putative transcriptional regulator . RolA, therefore, acts as a negative regulator of AI-2 production. Finally, it has been shown that the exogenous addition of AI-2 or 4-hydroxy-5-methyl-3(2) furanone has no discernible effect on the production of toxins by .

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2005-02-01
2019-11-13
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