RT Journal Article SR Electronic(1) A1 Alonso, R A1 Martín, A A1 Peláez, T A1 Marín, M A1 Rodríguez-Creixéms, M A1 Bouza, EYR 2005 T1 An improved protocol for pulsed-field gel electrophoresis typing of Clostridium difficile JF Journal of Medical Microbiology, VO 54 IS 2 SP 155 OP 157 DO https://doi.org/10.1099/jmm.0.45808-0 PB Microbiology Society, SN 1473-5644, AB Pulsed-field gel electrophoresis (PFGE) is the ‘gold standard’ technique for bacterial typing and has proved to be discriminatory and reproducible for typing Clostridium difficile. Nevertheless, a high proportion of strains are non-typable by this technique due to the degradation of the DNA during the process. The introduction of several modifications in the PFGE standard procedure increased typability from 40 % (90 isolates) to 100 % (220 isolates) while maintaining the high degree of discrimination and reproducibility of the technique., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.45808-0