Molecular typing of Clostridium difficile isolates from animals and humans may be useful for evaluation of the possibility for interspecies transmission. The objective of this study was to evaluate C. difficile isolates from domestic animals and humans using PCR ribotyping. Isolates were also tested using PCR for the presence of genes encoding toxins A and B. One hundred and thirty-three isolates of C. difficile from dogs (n = 92), horses (n = 21) and humans (n = 20), plus one each from a cat and a calf, were evaluated. Overall, 23 ribotypes were identified. Of these, nine were identified from dogs, 12 from horses, seven from humans and one each from the cat and calf. In dogs, humans and horses, one or two different ribotypes predominated. Overall, 25 % of isolates from humans were indistinguishable from isolates from one or more animal species. Genes encoding C. difficile toxins A and B were detected in all human, equine and bovine isolates, and in 69 % of canine isolates. While different ribotypes appear to predominate in different mammalian species, several indistinguishable strains may be found in multiple species. This suggests that there is a potential for interspecies transmission of C. difficile and epidemiological studies are warranted.
BarbutF.,
LalandeV.,
BurghofferB.,
ThienH. V.,
GrimprelE.,
PetitJ. C.2002; Prevalence and genetic characterization of toxin A variant strains of Clostridium difficile among adults and children with diarrhea in France. J Clin Microbiol 40:2079–2083[CrossRef]
BidetP.,
BarbutF.,
LalandeV.,
BurghofferB.,
PetitJ. C.1999; Development of a new PCR-ribotyping method for Clostridium difficile based on ribosomal RNA gene sequencing. FEMS Microbiol Lett 175:261–266[CrossRef]
CaveN. J.,
MarksS. L.,
KassP. H.,
MelliA. C.,
BrophyM. A.2002; Evaluation of a routine diagnostic fecal panel for dogs with diarrhea. J Am Vet Med Assoc 221:52–59[CrossRef]
FawleyW. N.,
FreemanJ.,
WilcoxM. H.2003; Evidence to support the existence of subgroups within the UK epidemic Clostridium difficile strain (PCR-ribotype 1). J Hosp Infect 54:74–77[CrossRef]
FrazierK. S.,
HerronA. J.,
HinesM. E.II,
GaskinJ. M.,
AltmanN. H.1993; Diagnosis of enteritis and enterotoxemia due to Clostridium difficile in captive ostriches ( Struthio camelus ). J Vet Diagn Invest 5:623–625[CrossRef]
KatoH.,
KatoN.,
WatanabeK. & 7 other authors; 2001; Analysis of Clostridium difficile isolates from nosocomial outbreaks at three hospitals in diverse areas of Japan. J Clin Microbiol 39:1391–1395[CrossRef]
MadewellB. R.,
BeaJ. K.,
KraegelS. A.,
WinthropM.,
TangY. J.,
SilvaJ.Jr1999; Clostridium difficile : a survey of fecal carriage in cats in a veterinary medical teaching hospital. J Vet Diagn Invest 11:50–54[CrossRef]
MarksS. L.,
KatherE. J.,
KassP. H.,
MelliA. C.2002; Genotypic and phenotypic characterization of Clostridium perfringens and Clostridium difficile in diarrheic and healthy dogs. J Vet Intern Med 16:533–540[CrossRef]
O'NeillG.,
AdamsJ. E.,
BowmanR. A.,
RileyT. V.1993; A molecular characterization of Clostridium difficile isolates from humans, animals and their environments. Epidemiol Infect 111:257–264[CrossRef]
O'NeillG. L.,
OgunsolaF. T.,
BrazierJ. S.,
DuerdenB. I.1996; Modification of a PCR ribotyping method for application as a routine typing scheme for Clostridium difficile
. Anaerobe 2:205–209[CrossRef]
PerkinsS. E.,
FoxJ. G.,
TaylorN. S.,
GreenD. L.,
LipmanN. S.1995; Detection of Clostridium difficile toxins from the small intestine and cecum of rabbits with naturally acquired enterotoxemia. Lab Anim Sci 45:379–384
StrubleA. L.,
TangY. J.,
KassP. H.,
GumerlockP. H.,
MadewellB. R.,
SilvaJ.Jr1994; Fecal shedding of Clostridium difficile in dogs: a period prevalence survey in a veterinary medical teaching hospital. J Vet Diagn Invest 6:342–347[CrossRef]
StubbsS. L. J.,
BrazierJ. S.,
O'NeillG. L.,
DuerdenB. I.1999; PCR targeted to the 16S–23S rRNA gene intergenic spacer region of Clostridium difficile and construction of a library consisting of 116 different PCR ribotypes. J Clin Microbiol 37:461–463
SvenungssonB.,
BurmanL. G.,
Jalakas-PornullK.,
LagergrenA.,
StruweJ.,
AkerlundT.2003; Epidemiology and molecular characterization of Clostridium difficile strains from patients with diarrhea: low disease incidence and evidence of limited cross-infection in a Swedish teaching hospital. J Clin Microbiol 41:4031–4037[CrossRef]
van den BergR. J.,
ClaasE. C.,
OyibD. H.,
KlaassenC. H.,
DijkshoornL.,
BrazierJ. S.,
KuijperE. J.2004; Characterization of toxin A-negative, toxin B-positive Clostridium difficile isolates from outbreaks in different countries by amplified fragment length polymorphism and PCR ribotyping. J Clin Microbiol 42:1035–1041[CrossRef]
WeeseJ. S.,
WeeseH. E.,
BourdeauT. L.,
StaempfliH. R.2001a; Suspected Clostridium difficile -associated diarrhea in two cats. J Am Vet Med Assoc 218:1436–1439[CrossRef]
WeeseJ. S.,
StaempfliH. R.,
PrescottJ. F.2001b; A prospective study of the roles of Clostridium difficile and enterotoxigenic Clostridium perfringens in equine diarrhoea. Equine Vet J 33:403–409