1887

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Volume 54, Issue 6

Phospholipase B enzyme expression is not associated with other virulence attributes in isolates from patients with human immunodeficiency virus infection Free

Abstract

The extracellular phospholipases of are considered to play a significant role in the pathogenesis of human infections. Therefore 30 clinical isolates of from human immunodeficiency virus (HIV)-infected individuals were screened for phospholipase production (using an egg-yolk-agar medium). Two groups of six isolates with positive (group A) or deficient (group B) phospholipase activity were then analysed for phospholipase B1 () gene expression both in egg-yolk-agar and yeast extract/peptone/dextrose (YPD) broth media. A total of four virulence attributes of these two groups were in turn characterized, namely their germ-tube formation, cell-surface-hydrophobicity (CSH), adhesion to buccal epithelial cells (ABEC) and haemolysin production, and these factors were subsequently correlated with expression. In the phospholipase-producing isolates (group A) a positive correlation was demonstrated between phospholipase production and the degree of expression in YPD medium ( = 0.96, < 0.01). No such association was observed in group A isolates for expression in egg-yolk-agar medium. Further, expression in egg-yolk agar was less than that in YPD medium, although a positive correlation was seen between the expression levels on regression analysis ( = 0.86, = 0.026). Surprisingly, however, no significant associations were observed in either growth media between expression and any of the four pathogenic attributes examined ( < 0.001). A significant correlation was seen between CSH and ABEC ( = 0.74) in group A isolates. The phospholipase-deficient group B, however, demonstrated a significant correlation between the latter parameters (r=+0^h.50) and also between germ-tube formation and ABEC (r=−0^h.59), and germ-tube formation and haemolysin production (r=+0^h.31). It appears that in oral isolates in HIV infection there may be no significant association between the degree of expression and other widely recognized major virulence attributes.

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2005-06-01
2024-12-04
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References

  1. Banno Y., Yamada T., Nozawa Y. 1985; Secreted phospholipases of the dimorphic fungus, Candida albicans ; separation of three enzymes and some biological properties. J Med Vet Mycol 23:47–54 [CrossRef]
     [Google Scholar]
  2. Barrett-Bee K., Hayes Y., Watson R. G., Ryley J. 1985; A comparison of phospholipase activity, cellular adherence and pathogenicity in yeast. J Gen Microbiol 131:1217–1221
     [Google Scholar]
  3. Borst A., Fluit A. C. 2003; High levels of hydrolytic enzymes secreted by Candida albicans isolates involved in respiratory infections. J Med Microbiol 52:971–974 [CrossRef]
     [Google Scholar]
  4. Calderone R. A., Fonzi W. A. 2001; Virulence factors of Candida albicans . Trends Microbiol 9:327–335 [CrossRef]
     [Google Scholar]
  5. Clark W. B., Bammann L. L., Gibbons R. J. 1978; Comparative estimates of bacterial affinities and adsorption sites on hydroxyapatite surfaces. Infect Immun 19:846–853
     [Google Scholar]
  6. Douglas L. J. 1987; Adhesion to surfaces. In The Yeasts vol2, 2nd edn. pp 239–280 Edited by Rose A. H, Harrison J. S. London: Academic Press;
     [Google Scholar]
  7. Espinel-Ingroff A., Vazquez J. A., Boikov D., Pfaller M. A. 1999; Evaluation of DNA-based typing procedures for strain categorization of Candida spp. Diagn Microbiol Infect Dis 33:231–239 [CrossRef]
     [Google Scholar]
  8. Fu Y., Ibrahim A. S., Fonzi W., Zhou X., Ramos C. F., Ghannoum M. A. 1997; Cloning and characterization of a gene ( LIPI ) which encodes a lipase from the pathogenic yeast Candida albicans . Microbiology 143:331–340 [CrossRef]
     [Google Scholar]
  9. Ghannoum M. A. 2000; Potential role of phospholipases in virulence and fungal pathogenesis. Clin Microbiol Rev 13:122–143 [CrossRef]
     [Google Scholar]
  10. Hoover C. I., Jantapour M. J., Newport G., Agabian N., Fisher S. J. 1998; Cloning and regulated expression of the Candida albicans phospholipase B ( PLB1 ) gene. FEMS Microbiol Lett 167:163–169 [CrossRef]
     [Google Scholar]
  11. Ibrahim A. S., Mirbod F., Filler S. G., Banno Y., Cole G. T., Kitajima Y., Edwards J. E. Jr, Nozawa Y., Ghannoum M. A. 1995; Evidence implicating phospholipase as a virulence factor of Candida albicans . Infect Immun 63:1993–1998
     [Google Scholar]
  12. Kantarcioglu A., Yucel A. 2002; Phospholipase and protease activities in clinical Candida isolates with reference to the sources of strains. Mycoses 45:160–165 [CrossRef]
     [Google Scholar]
  13. Kimura L. H., Pearsall N. N. 1978; Adherence of C.albicans to human buccal epithelial cells. Infect Immun 21:64–68
     [Google Scholar]
  14. Kothavade R. J., Panthaki M. H. 1998; Evaluation of phospholipase activity of Candida albicans and its correlation with pathogenicity in mice. J Med Microbiol 47:99–102 [CrossRef]
     [Google Scholar]
  15. Leidich S. D., Ibrahim A. S., Fu Y. & 8 other authors; 1998; Cloning and disruption of ca PLB1 , a phospholipase B gene involved in the pathogenicity of Candida albicans . J Biol Chem 273:26078–26086 [CrossRef]
     [Google Scholar]
  16. Luo G., Samaranayake L. P., Yau J. Y. Y. 2001; Candida species exhibit differential in vitro hemolytic activities. J Clin Microbiol 39:2971–2974 [CrossRef]
     [Google Scholar]
  17. MacFarlane T. W. 1990; Ecology and epidemiology of Candida . In Oral Candidosis pp 21–46 Edited by Samaranayake L. P, MacFarlane T. W. London: Wright;
     [Google Scholar]
  18. Macura A. B. 1987; Hydrophobicity of Candida albicans related to their adherence to mucosal epithelial cells. Zentralbl Bakteriol Mikrobiol Hyg [A] 266:491–496
     [Google Scholar]
  19. McCullough M. J., Ross B. C., Reade P. C. 1996; Candida albicans : a review of its history, taxonomy, epidemiology, virulence attributes, and methods of strain differentiation. Int J Oral Maxillofac Surg 25:136–144 [CrossRef]
     [Google Scholar]
  20. Mukherjee P. K., Seshan K. R., Leidich S. D., Chandra J., Cole G. T., Ghannoum M. A. 2001; Reintroduction of the PLB1 gene into Candida albicans restores virulence in vivo . Microbiology 147:2585–2597
     [Google Scholar]
  21. Odds F. C., Bernaerts R. 1994; CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species. J Clin Microbiol 32:1923–1929
     [Google Scholar]
  22. Panagoda G. J., Samaranayake L. P. 1998; The relationship between the cell length, adhesion to acrylic and relative cell surface hydrophobicity of Candida parapsilosis . Med Mycol 36:373–378 [CrossRef]
     [Google Scholar]
  23. Price M. F., Wilkinson I. D., Gentry L. O. 1982; Plate method for detection of phospholipase activity in Candida albicans . Sabouraudia 20:7–14 [CrossRef]
     [Google Scholar]
  24. Pugh D., Cawson R. A. 1975; The cytochemical localisation of phospholipase A and lysophospholipase in Candida albicans . Sabouraudia 13:110–115 [CrossRef]
     [Google Scholar]
  25. Ribeiro M. A., Miranda A. E., Gambale W., Claudete R. P. 2004; Prevalence and exoenzyme secretion by Candida albicans isolates from oral and vaginal mucosas of HIV-infected women. Mycopathologia 157:255–261 [CrossRef]
     [Google Scholar]
  26. Salyers A., Witt D. 1994; Virulence factors that damage the host. In Bacterial Pathogenesis: a Molecular Approach pp 47–62 Edited by Salyers A, Witt D. Washington, DC: ASM Press;
     [Google Scholar]
  27. Samaranayake L. P., Raeside J. M., MacFarlane T. W. 1984; Factors affecting the phospholipase activity of Candida species in vitro . J Med Vet Mycol 22:201–207 [CrossRef]
     [Google Scholar]
  28. Samaranayake L. P., MacFarlane T. W., Lamey P. J., Ferguson M. M. 1986; A comparison of oral rinse and imprint sampling techniques for the detection of yeast, coliform and Staphylococcus aureus carriage in the oral cavity. J Oral Pathol 15:386–388 [CrossRef]
     [Google Scholar]
  29. Samaranayake Y. H., Wu P. C., Samaranayake L. P. 1995; Relationship between the cell surface hydrophobicity and adherence of Candida krusei and Candida albicans to epithelial and denture acrylic surfaces. APMIS 103:707–713 [CrossRef]
     [Google Scholar]
  30. Samaranayake Y. H., Samaranayake L. P., Tsang P. C., Wong K. H., Yeung K. W. S. 2001; Heterogeneity in antifungal susceptibility of clones of Candida albicans isolated on single and sequential visits from a HIV-infected southern Chinese cohort. J Oral Pathol Med 30:336–346 [CrossRef]
     [Google Scholar]
  31. Schaller M., Schafer W., Korting H. C., Hube B. 1998; Differential expression of secreted aspartyl proteinases in a model of human oral candidosis and in patient samples from the oral cavity. Mol Microbiol 29:605–615 [CrossRef]
     [Google Scholar]
  32. Sneath P. H. A., Sokal R. R. 1973; Numerical taxonomy. In The Principles and Practice of Numerical Classification pp 230–234 San Francisco: W. H. Freeman;
     [Google Scholar]
  33. Soll D. R. 2000; The ins and outs of DNA fingerprinting the infectious fungi. Clin Microbiol Rev 13:332–370 [CrossRef]
     [Google Scholar]
  34. Sugiyama Y., Nakashima S., Mirbod F., Kanoh H., Kitajima Y., Ghannoum M. A., Nozawa Y. 1999; Molecular cloning of a second phospholipase B gene, caPLB2 from Candida albicans . Med Mycol 37:61–67
     [Google Scholar]
  35. Sweet S. P., MacFarlane T. W., Samaranayake L. P. 1987; Determination of cell surface hydrophobicity of oral bacteria using a modified hydrocarbon adherence method. FEMS Microbiol Lett 48:159–163 [CrossRef]
     [Google Scholar]
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Phospholipase B enzyme expression is not associated with other virulence attributes in Candida albicans isolates from patients with human immunodeficiency virus infection
J. Med. Microbiol. 54, 583 (2005); https://doi.org/10.1099/jmm.0.45762-0
/content/journal/jmm/10.1099/jmm.0.45762-0
/content/journal/jmm/10.1099/jmm.0.45762-0
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