1887

Abstract

The products of various strains of (BL21, DH5α, HB101 and XL Blue) were investigated for antimycotic properties using pathogenic isolates of . Co-culture experiments revealed that strains exhibited variable activity against . The lysates prepared from DH5α, HB101 and XL Blue strains of showed inhibitory activity against in the protein concentration range of 62.50 to 250.00 μg ml. The highest activity was seen in the lysate of BL21, which inhibited the growth of and completely at a concentration of 31.25 μg protein ml. The MIC of BL21 lysate against was found to be 62.50 μg ml. The toxicity of BL21 lysate was evaluated using a haemolytic assay. A BL21 lysate protein concentration of 1250.00 μg ml was found to be nontoxic to human erythrocytes. The standard drug amphotericin B lysed 100 % of erythrocytes at a concentration of 37.50 μg ml. SDS-PAGE showed the presence of at least 15 major proteins in the lysate of BL21. Ion-exchange chromatography resolved the BL21 lysate into five fractions and fraction III was found to be endowed with anti- properties. The MIC of this fraction was found to be 3.90 μg ml. Further work on the purification of the active molecule and its characterization is in progress.

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2005-04-01
2019-11-18
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