1887

Abstract

A screw-capped glass tube containing a culture accidentally broke and the laboratory worker who was handling the tube sustained a cut on his hand. The wound was flooded with the culture. The culture was that of strain MG 347 belonging to serovar Australis recovered from a patient, and it had undergone 52 passages in Ellinghausen McCullough Johnson Harris medium. The laboratory worker developed a headache 21 days after the accident and became febrile the next day. He was hospitalized for 5 days and was treated initially with doxycycline and later with ciprofloxacin. A blood sample collected on the second day of illness, after starting doxycycline therapy, yielded leptospires and the isolate, HZ 651, was identified as serovar Australis. Monoclonal antibody patterns and randomly amplified polymorphic DNA fingerprinting patterns of the isolate and strain MG 347 were identical, thus indicating that HZ 651 and MG 347 were clonal.

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2004-12-01
2024-12-03
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References

  1. Brown P. D., Levett P. N. 1997; Differentiation of Leptospira species and serovars by PCR-restriction endonuclease analysis, arbitrarily primed PCR and low-stringency PCR. J Med Microbiol 46:173–181 [CrossRef]
    [Google Scholar]
  2. Campagnolo E. R., Warwick M. C., Marx H. L. Jr 9 other authors; 2000; Analysis of the 1998 outbreak of leptospirosis in Missouri in humans exposed to infected swine. J Am Vet Med Assoc 216:676–682 [CrossRef]
    [Google Scholar]
  3. Faine S., Adler B., Bolin C., Perolat P. 1999 Leptospira and Leptospirosis , 2nd edn. Melbourne: MedSci;
    [Google Scholar]
  4. Gilks C. F., Lambert H. P., Broughton E. S., Baker C. C. 1988; Failure of penicillin prophylaxis in laboratory acquired leptospirosis. Postgrad Med J 64:236–238 [CrossRef]
    [Google Scholar]
  5. Gussenhoven G. C., Hoorn M. A. W. G., Goris M. G. A., Terpstra W. J., Hartskeerl R. A., Mol B. W., van Ingen C. W., Smits H. L. 1997; LEPTO dipstick, a dipstick assay for detection of Leptospira -specific immunoglobulin M antibodies in human sera. J Clin Microbiol 35:92–97
    [Google Scholar]
  6. Natarajaseenivasan K., Boopalan M., Selvanayaki K., Suresh S. R., Ratnam S. 2002; Leptospirosis among rice mill workers of Salem, South India. Jpn J Infect Dis 55:170–173
    [Google Scholar]
  7. Schwabe C. W. 1984; Zoonoses. In Veterinary Medicine and Human Health . , 3rd edn. pp 199–207 Edited by Stamathis G. Baltimore: Williams & Wilkins;
  8. Sehgal S. C., Vijayachari P., Smythe L. D. & 7 other authors; 2000; Lai-like leptospira from the Andaman Islands. Ind J Med Res 112:135–139
    [Google Scholar]
  9. Sullivan J. F., Songer J. R., Estrem I. E. 1978; Laboratory-acquired infections at the National Animal Disease Center 1960–1976. Health Lab Sci 15:58–64
    [Google Scholar]
  10. Turner L. H. 1967; Leptospirosis.I. Trans R Soc Trop Med Hyg 61:842–854 [CrossRef]
    [Google Scholar]
  11. Vijayachari P., Sehgal S. C., Goris M. G. A., Terpstra W. J., Hartskeerl R. A. 2003; Leptospira interrogans serovar Valbuzzi: a cause of severe pulmonary haemorrhages in the Andaman Islands. J Med Microbiol 52:1–6 [CrossRef]
    [Google Scholar]
  12. Wolff J. W. 1954; Serological procedures. In Laboratory Diagnosis of Leptospirosis pp 39–62 Springfield, IL: Charles C. Thomas;
    [Google Scholar]
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