1887

Abstract

Group A streptococcus (GAS) infection can cause severe invasive diseases, including necrotizing fasciitis and streptococcal toxic shock syndrome. , a Chinese herbal medicine, is an immunomodulator. In this study the air-pouch bacterial inoculation model was used to investigate the protective efficacy of mycelium extract against GAS infection. Force-feeding mice with mycelium extract for 3 consecutive days before GAS infection increased the survival rate and reduced local skin-tissue injury compared with mice fed PBS. Bacterial numbers in the air pouch exudates from -treated mice were lower than those from PBS-treated mice. Blood and organs in PBS-treated mice showed bacterial dissemination, but those in -treated mice did not. Three days of pretreatment with extract followed by treatment every other day after GAS infection resulted in 100 % survival. The post-GAS-infection levels of aspartate aminotransferase, alanine aminotransferase and blood urea nitrogen in the sera of -treated mice were lower than those of PBS-treated mice. Taken together, these results show that mycelium extract protects by decreasing bacterial growth and dissemination, thereby increasing mouse survival rate. IL-12 and IFN-γ expression and macrophage phagocytic activity also increased after treatment.

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2005-08-01
2021-03-08
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References

  1. Bisno A. L., Brito M. O., Collins C. M. 2003; Molecular basis of group A streptococcal virulence. Lancet Infect Dis 3:191–200 [CrossRef]
    [Google Scholar]
  2. Chen Y. J., Shiao M. S., Lee S. S., Wang S. Y. 1997; Effect of Cordyceps sinensis on the proliferation and differentiation of human leukemic U937 cells. Life Sci 60:2349–2359 [CrossRef]
    [Google Scholar]
  3. Chen C. Y., Luo S. C., Kuo C. F., Lin Y. S., Wu J. J., Lin M. T., Liu C. C., Jeng W. Y., Chuang W. J. 2003; Maturation processing and characterization of streptopain. J Biol Chem 278:17336–17343 [CrossRef]
    [Google Scholar]
  4. Cunningham M. W. 2000; Pathogenesis of group A streptococcal infections. Clin Microbiol Rev 13:470–511 [CrossRef]
    [Google Scholar]
  5. Efstratiou A. 2000; Group A streptococci in the 1990s. J Antimicrob Chemother 45:Topic T13–12
    [Google Scholar]
  6. Kagawa T. F., Cooney J. C., Baker H. M., McSweeney S., Liu M., Gubba S., Musser J. M., Baker E. N. 2000; Crystal structure of the zymogen form of the group A Streptococcus virulence factor SpeB: an integrin-binding cysteine protease. Proc Natl Acad Sci U S A 97:2235–2240 [CrossRef]
    [Google Scholar]
  7. Kuo Y. C., Lin C. Y., Tsai W. J., Wu C. L., Chen C. F., Shiao M. S. 1994; Growth inhibitors against tumor cells in Cordyceps sinensis other than cordycepin and polysaccharides. Cancer Invest 12:611–615 [CrossRef]
    [Google Scholar]
  8. Kuo C. F., Wu J. J., Lin K. Y., Tsai P. J., Lee S. C., Jin Y. T., Lei H. Y., Lin Y. S. 1998; Role of streptococcal pyrogenic exotoxin B in the mouse model of group A streptococcal infection. Infect Immun 66:3931–3935
    [Google Scholar]
  9. Kuo C. F., Wu J. J., Tsai P. J., Kao F. J., Lei H. Y., Lin M. T., Lin Y. S. 1999; Streptococcal pyrogenic exotoxin B induces apoptosis and reduces phagocytic activity in U937 cells. Infect Immun 67:126–130
    [Google Scholar]
  10. Kuo Y. C., Tsai W. J., Wang J. Y., Chang S. C., Lin C. Y., Shiao M. S. 2001; Regulation of bronchoalveolar lavage fluids cell function by the immunomodulatory agents from Cordyceps sinensis . Life Sci 68:1067–1082 [CrossRef]
    [Google Scholar]
  11. Kuo C. F., Luo Y. H., Lin H. Y. & 8 other authors; 2004; Histopathologic changes in kidney and liver correlate with streptococcal pyrogenic exotoxin B production in the mouse model of group A streptococcal infection. Microb Pathog 36:273–285 [CrossRef]
    [Google Scholar]
  12. Lin C. F., Lei H. Y., Shiau A. L., Liu H. S., Yeh T. M., Chen S. H., Liu C. C., Chiu S. C., Lin Y. S. 2002; Endothelial cell apoptosis induced by antibodies against dengue virus nonstructural protein 1 via production of nitric oxide. J Immunol 169:657–664 [CrossRef]
    [Google Scholar]
  13. Lukomski S., Burns E. H. Jr, Wyde P. R., Podbielski A., Rurangirwa J., Moore-Poveda D. K., Musser J. M. 1998; Genetic inactivation of an extracellular cysteine protease (SpeB) expressed by Streptococcus pyogenes decreases resistance to phagocytosis and dissemination to organs. Infect Immun 66:771–776
    [Google Scholar]
  14. Medina E., Goldmann O., Rohde M., Lengeling A., Chhatwals G. S. 2001; Genetic control of susceptibility to group A streptococcal infection in mice. J Infect Dis 184:846–852 [CrossRef]
    [Google Scholar]
  15. Metzger D. W., Raeder R., Van Cleave V. H., Boyle M. D. P. 1995; Protection of mice from group A streptococcal skin infection by interleukin-12. J Infect Dis 171:1643–1645 [CrossRef]
    [Google Scholar]
  16. Raeder R. H., Barker-Merrill L., Lester T., Boyle M. D. P., Metzger D. W. 2000; A pivotal role for interferon-γ in protection against group A streptococcal skin infection. J Infect Dis 181:639–645 [CrossRef]
    [Google Scholar]
  17. Rasmussen M., Bjorck L. 2002; Proteolysis and its regulation at the surface of Streptococcus pyogenes . Mol Microbiol 43:537–544 [CrossRef]
    [Google Scholar]
  18. Shimitsu D. 1978 Green Book 51, Cordyceps Japan: New Science Company;
    [Google Scholar]
  19. Yamaguchi N., Yoshida J., Ren L. J. & 8 other authors; 1990; Augmentation of various immune reactivities of tumor-bearing hosts with an extract of Cordyceps sinensis . Biotherapy 2:199–205 [CrossRef]
    [Google Scholar]
  20. Yoshida J., Takamura S., Yamaguchi N., Ren L. J., Chen H., Koshimura S., Suzuki S. 1989; Antitumor activity of an extract of Cordyceps sinensis (Berk.) Sacc. against murine tumor cell lines. Jpn J Exp Med 59:157–161
    [Google Scholar]
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