1887

Abstract

Traditional methods of identification of food-borne pathogens, which cause disease in humans, are time-consuming and laborious, so there is a need for the development of innovative methods for the rapid identification of food-borne pathogens. Recent advances in molecular cloning and recombinant DNA techniques have revolutionized the detection of pathogens in foods. In this study the development of a PCR-based technique for the rapid identification of the food-borne pathogens and was undertaken. Suitable primers were designed based on specific gene of and gene of pathogenic for amplification. Agarose gel electrophoresis and subsequent staining with ethidium bromide were used for the identification of PCR products. The size of the amplified product was 120 bp as shown by comparison with marker DNA. These studies have established that and primers were specific for detecting and pathogenic , respectively, in the environmental samples. Thus a rapid, sensitive and reliable technique for the detection of and pathogenic was developed.

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2005-01-01
2019-11-18
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