Role of quorum sensing in the pathogenicity of Free

Abstract

is the causative agent of human and animal melioidosis. The role of quorum sensing (QS) in the pathogenicity of via inhalational exposure of BALB/c mice and intraperitoneal challenge of Syrian hamsters has not been reported. This investigation demonstrates that encodes a minimum of three and five homologues that are involved in animal pathogenicity. Mass spectrometry analysis of culture supernatants revealed that wild-type and the mutants synthesized numerous signalling molecules, including -octanoyl-homoserine lactone, -decanoyl-homoserine lactone, -(3-hydroxyoctanoyl)--homoserine lactone, -(3-hydroxydecanoyl)--homoserine lactone and -(3-oxotetradecanoyl)--homoserine lactone, which was further confirmed by heterologous expression of the alleles in . Mutagenesis of the QS system increased the time to death and reduced organ colonization of aerosolized BALB/c mice. Further, intraperitoneal challenge of Syrian hamsters with the QS mutants resulted in a significant increase in the LD Using semi-quantitative plate assays, preliminary analysis suggests that QS does not affect lipase, protease and phospholipase C biosynthesis/secretion in . The findings of the investigation demonstrate that encodes multiple genes, and disruption of the QS alleles reduces animal pathogenicity, but does not affect exoproduct secretion.

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2004-11-01
2024-03-29
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