1887

Abstract

is recognized as the cause of a severe life-threatening illness characterized by bacteraemia with metastatic abscesses following an acute sore throat (Lemierre's disease). However, the importance of as a cause of simple sore throat in the community is unknown. Using quantitative real-time PCR with primers targeting the gene, 100 routine throat swabs collected from patients presenting to general practitioners with pharyngitis were analysed for the presence of -specific DNA. The results were compared with those obtained from throat swabs collected from 100 healthy subjects. Ten clinical samples were positive for DNA, identified as subspecies , using a haemagglutinin-related protein gene-specific PCR assay. All the healthy controls were negative (two-tailed value = 0.0015; Fisher exact test). These findings suggest that may play a more important role as a cause of simple sore throat in the community than has been previously appreciated.

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2004-10-01
2020-10-22
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References

  1. Alvarez A., Schreiber J. R. 1995; Lemierre's syndrome in adolescent children – anaerobic sepsis with internal jugular vein thrombophlebitis following pharyngitis. Pediatrics 96:354–359
    [Google Scholar]
  2. Baddour L. M., Land M. A., Barrett F. F., Rivara F. P., Bruce W. M., Bisno A. L. 1986; Hepatobiliary abnormalities associated with postanginal sepsis.Common manifestations of an uncommon disease. Diagn Microbiol Infect Dis 4:19–28 [CrossRef]
    [Google Scholar]
  3. Bartlett J. G., Finegold S. M. 1972; Anaerobic pleuropulmonary infections. Medicine (Baltimore) 51:413–450
    [Google Scholar]
  4. Beerens H., Fievez L., Wattre P. 1971; Observations concernant 7 souches appartenant aux especes Saphaerophorus necrophorus , Sphaerophorus funduliformis , Sphaerophorus pseudonecrophorus . Ann Inst Pasteur (Paris) 121:37–41 (in French
    [Google Scholar]
  5. Bisno A. L. 2001; Acute pharyngitis. N Engl J Med 344:205–211 [CrossRef]
    [Google Scholar]
  6. Boom R., Sol C. J., Salimans M. M., Jansen C. L., Wertheim-van Dillen P. M., van der Noordaa J. 1990; Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28:495–503
    [Google Scholar]
  7. Bourbeau P. P. 2003; Role of the microbiology laboratory in diagnosis and management of pharyngitis. J Clin Microbiol 41:3467–3472 [CrossRef]
    [Google Scholar]
  8. Brazier J. S. 2002; Fusobacterium necrophorum infections in man. Rev Med Microbiol 13:141–149 [CrossRef]
    [Google Scholar]
  9. Brazier J. S., Hall V., Yusuf E., Duerden B. I. 2002; Fusobacterium necrophorum infections in England and Wales 1990–2000. J Med Microbiol 51:269–272
    [Google Scholar]
  10. Dagan R., Powell K. R. 1987; Postanginal sepsis following infectious mononucleosis. Arch Intern Med 147:1581–1583 [CrossRef]
    [Google Scholar]
  11. Department of Health Standing Medical Advisory Committee 1998 Sub-group on Antimicrobial Resistance Occasional Report. The path of least resistance London, UK: The Stationery Office;
    [Google Scholar]
  12. Hagelskjaer Kristensen L., Prag J. 2000; Human necrobacillosis, with emphasis on Lemierre's syndrome. Clin Infect Dis 31:524–532 [CrossRef]
    [Google Scholar]
  13. Hagelskjaer L. H., Prag J., Malczynski J., Kristensen J. H. 1998; Incidence and clinical epidemiology of necrobacillosis, including Lemierre's syndrome, in Denmark 1990–1995. Eur J Clin Microbiol Infect Dis 17:561–565
    [Google Scholar]
  14. Hoffmann S. 1986; Incidence and management of sore throat in general practice. Scand J Prim Health Care 4:143–150 [CrossRef]
    [Google Scholar]
  15. Jones J. W., Riordan T., Morgan M. S. 2001; Investigation of postanginal sepsis and Lemierre's syndrome in the South West Peninsula. Commun Dis Public Health 4:278–281
    [Google Scholar]
  16. Lemierre A. 1936; On certain septicaemias. Lancet 1:701–703
    [Google Scholar]
  17. Moore-Gillon J., Lee T. H., Eykyn S. J., Phillips I. 1984; Necrobacillosis: a forgotten disease. Br Med J (Clin Res Ed) 288:1526–1527 [CrossRef]
    [Google Scholar]
  18. Narayanan S. K., Nagaraja T. G., Chengappa M. M., Stewart G. C. 2001; Cloning, sequencing, and expression of the leukotoxin gene from Fusobacterium necrophorum . Infect Immun 69:5447–5455 [CrossRef]
    [Google Scholar]
  19. Narongwanichgarn W., Misawa N., Jin J. H., Amoako K. K., Kawaguchi E., Shinjo T., Haga T., Goto Y. 2003; Specific detection and differentiation of two subspecies of Fusobacterium necrophorum by PCR. Vet Microbiol 91:183–195 [CrossRef]
    [Google Scholar]
  20. Sherer Y., Mishal J., Leibovici O. 2000; Early antibiotic treatment may prevent complete development of Lemierre's syndrome: experience from 2 cases. Scand J Infect Dis 32:706–707 [CrossRef]
    [Google Scholar]
  21. Shinjo T., Fujisawa T., Mitsuoka T. 1991; Proposal of two subspecies of Fusobacterium necrophorum (Flügge) Moore and Holdeman: Fusobacterium necrophorum subsp. necrophorum subsp. nov., nom. rev. (ex Flügge 1886), and Fusobacterium necrophorum subsp. funduliforme subsp. nov., nom. rev. (ex Halle 1898). Int J Syst Bacteriol 41:395–397 [CrossRef]
    [Google Scholar]
  22. Sinave C. P., Hardy G. J., Fardy P. W. 1989; The Lemierre syndrome: suppurative thrombophlebitis of the internal jugular vein secondary to oropharyngeal infection. Medicine (Baltimore) 68:85–94
    [Google Scholar]
  23. Smith G. R., Thornton E. A. 1993; Pathogenicity of Fusobacterium necrophorum strains from man and animals. Epidemiol Infect 110:499–506 [CrossRef]
    [Google Scholar]
  24. Tan Z. L., Nagaraja T. G., Chengappa M. M., Smith J. S. 1994; Biological and biochemical characterization of Fusobacterium necrophorum leukotoxin. Am J Vet Res 55:515–521
    [Google Scholar]
  25. Tan Z. L., Nagaraja T. G., Chengappa M. M. 1996; Fusobacterium necrophorum infections: virulence factors, pathogenic mechanism and control measures. Vet Res Commun 20:113–140 [CrossRef]
    [Google Scholar]
  26. Weesner C. L., Cisek J. E. 1993; Lemierre syndrome: the forgotten disease. Ann Emerg Med 22:256–258 [CrossRef]
    [Google Scholar]
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