1887

Abstract

Validation of a PCR test to detect gene sequences of spp. was performed in blood and faeces samples from typhoid fever and salmonellosis patients. Sensitivity (S), specificity (SP), positive predictive value (PPV) and negative predictive value (NPV) of the PCR in blood samples were performed by testing: 37 patients with clinical diagnosis of typhoid fever, 34 of them confirmed by isolation of . Typhi from blood cultures; 35 patients infected with other pathogens corroborated by blood culture (, 9; , 5; , 4; , 9; , 4 and , 4) and blood samples from 150 healthy volunteers. To evaluate S, SP, PPV and NPV of the PCR in faeces samples we studied: 34 patients with enteritis due spp. (. Typhimurium, 21; . Enteritidis, 9; . Choleraesuis, 3 and . Agona, 1); faeces samples from 35 patients with enteric infection due to (8), (10), enteropathogenic (12), (5) and faeces samples from 150 healthy volunteers. The S, SP, PPV and NPV of the PCR in blood samples were all 100 %. PCR detected three patients with clinical diagnosis of typhoid fever and negative blood cultures. In faeces samples, S was 97 %, SP 100 %, PPV 100 % and NPV 99 %. The lowest number of c.f.u. ml detected by PCR in blood samples was 1×10 and in faeces samples 4×10.

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2004-09-01
2019-11-18
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