Bartonella henselae is a recently recognized pathogenic bacterium associated with cat-scratch disease, bacillary angiomatosis and bacillary peliosis. A recombinant clone expressing an immunoreactive antigen of B. henselae was isolated by screening a genomic DNA cosmid library by Western blotting with sera pooled from patients positive for B. henselae IgG antibodies by indirect immunofluorescence (IFA). The deduced amino acid sequence of the 43.7 kDa encoded protein was found to be 76.3 % identical to the dihydrolipoamide succinyltransferase enzyme (SucB) of Brucella melitensis. SucB has been shown to be an immunogenic protein during infections by Brucella melitensis, Coxiella burnetii and Bartonella vinsonii. The agreement between reactivity with a recombinant SucB fusion protein on immunoblot analysis and the results obtained by IFA was 55 % for IFA-positive sera and 88 % for IFA-negative sera. Cross-reactivity was observed with sera from patients with antibodies against Brucella melitensis, Mycoplasma pneumoniae, Francisella tularensis, Coxiella burnetii and Rickettsia typhi.
AndersonB.,
LuE.,
JonesD.,
RegneryR.1995; Characterization of a 17-kilodalton antigen of Bartonella henselae reactive with sera from patients with cat scratch disease. J Clin Microbiol 33:2358–2365
AndersonB.,
JonesD.,
BurgessA.1996; Cloning, expression and sequence analysis of the Bartonella henselae gene encoding the HtrA stress-response protein. Gene 178:35–38[CrossRef]
BradfordM. M.1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248–254[CrossRef]
DaltonM. J.,
RobinsonL. E.,
CooperJ.,
RegneryR. L.,
OlsonJ. G.,
ChildsJ. E.1995; Use of Bartonella antigens for serologic diagnosis of cat-scratch disease at a national referral center. Arch Intern Med 155:1670–1676[CrossRef]
GilmoreR. D.Jr,
CarpioA. M.,
KosoyM. Y.,
GageK. L.2003; Molecular characterization of the sucB gene encoding the immunogenic dihydrolipoamide succinyltransferase protein of Bartonella vinsonii subsp. berkhoffii and Bartonella quintana
. Infect Immun 71:4818–4822[CrossRef]
KoehlerJ. E.,
GlaserC. A.,
TapperoJ. W.1994; Rochalimaea henselae infection.A new zoonosis with the domestic cat as reservoir. JAMA 271:531–535[CrossRef]
LiangZ.,
RaoultD.2000; Differentiation of Bartonella species by a microimmunofluorescence assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western immunoblotting. Clin Diagn Lab Immunol 7:617–624
LitwinC. M.,
MartinsT. B.,
HillH. R.1997; Immunologic response to Bartonella henselae as determined by enzyme immunoassay and Western blot analysis. Am J Clin Pathol 108:202–209
MaurinM.,
RouxV.,
SteinA.,
FerrierF.,
VirabenR.,
RaoultD.1994; Isolation and characterization by immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western blot, restriction fragment length polymorphism-PCR, 16S rRNA gene sequencing, and pulsed-field gel electrophoresis of Rochalimaea quintana from a patient with bacillary angiomatosis. J Clin Microbiol 32:1166–1171
MaurinM.,
RolainJ. M.,
RaoultD.2002; Comparison of in-house and commercial slides for detection by immunofluorescence of immunoglobulins G and M against Bartonella henselae and Bartonella quintana
. Clin Diagn Lab Immunol 9:1004–1009
ReedJ. A.,
BrigatiD. J.,
FlynnS. D.,
McNuttN. S.,
MinK. W.,
WelchD. F.,
SlaterL. N.1992; Immunocytochemical identification of Rochalimaea henselae in bacillary (epithelioid) angiomatosis, parenchymal bacillary peliosis, and persistent fever with bacteremia. Am J Surg Pathol 16:650–657[CrossRef]
SanderA.,
PosseltM.,
OberleK.,
BredtW.1998; Seroprevalence of antibodies to Bartonella henselae in patients with cat scratch disease and in healthy controls: evaluation and comparison of two commercial serological tests. Clin Diagn Lab Immunol 5:486–490
TuuminenT.,
VarjoS.,
IngmanH.,
WeberT.,
OksiJ.,
ViljanenM.2000; Prevalence of Chlamydia pneumoniae and Mycoplasma pneumoniae immunoglobulin G and A antibodies in a healthy Finnish population as analyzed by quantitative enzyme immunoassays. Clin Diagn Lab Immunol 7:734–738
ZangwillK. M.,
HamiltonD. H.,
PerkinsB. A.,
RegneryR. L.,
PlikaytisB. D.,
HadlerJ. L.,
CartterM. L.,
WengerJ. D.1993; Cat scratch disease in Connecticut.Epidemiology, risk factors, and evaluation of a new diagnostic test. N Engl J Med 329:8–13[CrossRef]
ZygmuntM. S.,
DiazM. A.,
Teixeira-GomesA. P.,
CloeckaertA.2001; Cloning, nucleotide sequence, and expression of the Brucella melitensis sucB gene coding for an immunogenic dihydrolipoamide succinyltransferase homologous protein. Infect Immun 69:6537–6540[CrossRef]