1887

Abstract

Indigenous intestinal microflora (including lactobacilli) has an important role in protection against infection. To assess interaction between lactobacilli and , antagonistic activity of 50 intestinal spp. strains against 23 pathogenic strains was determined. Phenotypic properties of strains [production of short-chain fatty acids (SCFAs) and toxin A, and antimicrobial susceptibility] and lactobacilli (production of SCFAs and HO) were investigated. Five lactobacilli ( and species) were antagonistic to all strains, 18 were antagonistic to some strains and 27 showed no antagonistic activity. This antagonistic activity was strain-specific and seemed to correlate with HO and lactic acid production. strains that were more sensitive to lactobacilli ( = 9) usually produced higher toxin levels and more SCFAs, and were more resistant to antibiotics, than strains that were resistant to lactobacilli ( = 14). Compatibility of strain properties (resistance to lactobacilli or antibiotics) with intestinal microecological conditions (e.g. presence of antagonistic lactobacilli, concentration of antibiotics) may determine expression of disease.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.45595-0
2004-06-01
2019-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jmm/53/6/JM530614.html?itemId=/content/journal/jmm/10.1099/jmm.0.45595-0&mimeType=html&fmt=ahah

References

  1. Bartlett, J. G. ( 1992;). Antibiotic-associated diarrhea. Clin Infect Dis 15, 573–581.[CrossRef]
    [Google Scholar]
  2. Biller, J. A., Katz, A. J., Flores, A. F., Buie, T. M. & Gorbach, S. L. ( 1995;). Treatment of recurrent Clostridium difficile colitis with Lactobacillus GG. J Pediatr Gastroenterol Nutr 21, 224–226.[CrossRef]
    [Google Scholar]
  3. Bogovic-Matijašić, B., Rogelj, I., Nes, I. F. & Holo, H. ( 1998;). Isolation and characterization of two bacteriocins of Lactobacillus acidophilus LF221. Appl Microbiol Biotechnol 49, 606–612.[CrossRef]
    [Google Scholar]
  4. Brashears, M. M. & Durre, W. A. ( 1999;). Antagonistic action of Lactobacillus lactis toward Salmonella spp.and Escherichia coli O157 : H7 during growth and refrigerated storage. J Food Prot 62, 1336–1340.
    [Google Scholar]
  5. Brashears, M. M., Reilly, S. S. & Gilliland, S. E. ( 1998;). Antagonistic action of cells of Lactobacillus lactis toward Escherichia coli O157 : H7 on refrigerated chicken meat. J Food Prot 61, 166–170.
    [Google Scholar]
  6. Coconnier, M.-H., Liévin, V., Bernet-Camard, M.-F., Hudault, S. & Servin, A. L. ( 1997;). Antibacterial effect of the adhering human Lactobacillus acidophilus strain LB. Antimicrob Agents Chemother 41, 1046–1052.
    [Google Scholar]
  7. D'Souza, A. L., Rajkumar, C., Cooke, J. & Bulpitt, C. J. ( 2002;). Probiotics in prevention of antibiotic associated diarrhoea: meta-analysis. BMJ 324, 1361–1366.[CrossRef]
    [Google Scholar]
  8. Eschenbach, D. A., Davick, P. R., Williams, B. L., Klebanoff, S. J., Young-Smith, K., Critchlow, C. M. & Holmes, K. K. ( 1989;). Prevalence of hydrogen peroxide-producing Lactobacillus species in normal women and women with bacterial vaginosis. J Clin Microbiol 27, 251–256.
    [Google Scholar]
  9. Forestier, C., De Champs, C., Vatoux, C. & Joly, B. ( 2001;). Probiotic activities of Lactobacillus casei rhamnosus: in vitro adherence to intestinal cells and antimicrobial properties. Res Microbiol 152, 167–173.[CrossRef]
    [Google Scholar]
  10. Holdeman, L. V. & Moore, W. E. C. (editors) ( 1972;). Anaerobe Laboratory Manual. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  11. Johnson, S. & Gerding, D. N. ( 1998;). Clostridium difficile-associated diarrhea. Clin Infect Dis 26, 1027–1036.[CrossRef]
    [Google Scholar]
  12. Kotz, C. M., Peterson, L. R., Moody, J. A., Savaiano, D. A. & Levitt, M. D. ( 1992;). Effect of yogurt on clindamycin-induced Clostridium difficile colitis in hamsters. Dig Dis Sci 37, 129–132.[CrossRef]
    [Google Scholar]
  13. Malakar, P. K., Martens, D. E., Zwietering, M. H., Béal, C. & van't Riet, K. ( 1999;). Modelling the interactions between Lactobacillus curvatus and Enterobacter cloacae.II. Mixed cultures and shelf life predictions. Int J Food Microbiol 51, 67–79.[CrossRef]
    [Google Scholar]
  14. Martínez, J. L. & Baquero, F. ( 2002;). Interactions among strategies associated with bacterial infection: pathogenicity, epidemicity, and antibiotic resistance. Clin Microbiol Rev 15, 647–679.[CrossRef]
    [Google Scholar]
  15. McFarland, L. V. ( 1998;). Epidemiology, risk factors and treatments for antibiotic-associated diarrhea. Dig Dis 16, 292–307.[CrossRef]
    [Google Scholar]
  16. Mikelsaar, M., Annuk, H., Shchepetova, J., Mändar, R., Sepp, E. & Björkstén, B. ( 2002;). Intestinal lactobacilli of Estonian and Swedish children. Microb Ecol Health Dis 14, 75–80.[CrossRef]
    [Google Scholar]
  17. Naaber, P. ( 1997;). Clostridium difficile infection and intestinal microbial ecology. PhD thesis, University of Tartu, Tartu, Estonia.
  18. Naaber, P., Klaus, K., Sepp, E., Björksten, B. & Mikelsaar, M. ( 1997;). Colonization of infants and hospitalized patients with Clostridium difficile and lactobacilli. Clin Infect Dis 25, S189–S190.[CrossRef]
    [Google Scholar]
  19. Ouwehand, A. C. ( 1998;). Antimicrobial components from lactic acid bacteria. In Lactic Acid Bacteria: Microbiology and Functional Aspects, 2nd edn, pp. 139–160. Edited by S. Salminen & A. von Wright. New York: Marcel Dekker.
  20. Pochapin, M. ( 2000;). The effect of probiotics on Clostridium difficile diarrhea. Am J Gastroenterol 95, S11–S13.[CrossRef]
    [Google Scholar]
  21. Roffe, C. ( 1996;). Biotherapy for antibiotic-associated and other diarrhoeas. J Infect 32, 1–10.[CrossRef]
    [Google Scholar]
  22. Strus, M., Pakosz, K., Gosciniak, H., Przondo-Mordarska, A., Rozynek, E., Pituch, H., Meisel-Mikolajczyk, F. & Heczko, P. B. ( 2001;). Antagonistic activity of Lactobacillus bacteria strains against anaerobic gastrointestinal tract pathogens (Helicobacter pylori, Campylobacter coli, Campylobacter jejuni, Clostridium difficile). Med Dosw Mikrobiol 53, 133–142 (in Polish).
    [Google Scholar]
  23. Su, W. J., Waechter, M. J., Bourlioux, P., Dolegeal, M., Fourniat, J. & Mahuzier, G. ( 1987;). Role of volatile fatty acids in colonization resistance to Clostridium difficile in gnotobiotic mice. Infect Immun 55, 1686–1691.
    [Google Scholar]
  24. Tang, Y. J., Houston, S. T., Gumerlock, P. H., Mulligan, M. E., Gerding, D. N., Johnson, S., Fekety, F. R. & Silva, J., Jr ( 1995;). Comparison of arbitrarily primed PCR with restriction endonuclease and immunoblot analyses for typing Clostridium difficile isolates. J Clin Microbiol 33, 3169–3173.
    [Google Scholar]
  25. Vandenplas, Y. ( 1999;). Bacteria and yeasts in the treatment of acute and chronic infectious diarrhea.Part I. Bacteria. Clin Microbiol Infect 5, 299–307.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.45595-0
Loading
/content/journal/jmm/10.1099/jmm.0.45595-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error