1887

Abstract

A PCR assay for the detection of and was compared with the conventional culture method under routine laboratory conditions. Detection of was based on the amplification of a section of the IS insertion sequence and confirmation of positive results was based on a sequence of the pertussis toxin promoter region. Detection of was based on the amplification of a section of the IS insertion sequence. An internal control was included. Data were available for the period 28 November 2000 to 9 July 2003. In this period, 3096 patients were examined for infection with and by culture and PCR on the same day. was found in 496 (16 %) patients; 208 (42 %) were diagnosed by PCR alone whereas 17 (3 %) were diagnosed by culture alone. was found in 64 (2 %) patients. The sensitivity of the PCR was 97 % and of culture 58 %. The specificity of PCR was 93 % when regarding culture as 100 % sensitive. There was a significant relationship between laboratory method and age, as the superiority of PCR was most marked in the age group 0.5–3 years. The PCR assay proved highly sensitive for the diagnosis of pertussis. The specificity estimate of the PCR assay suffers from the influence of a gold-standard method with a low sensitivity. The PCR assay is considered highly specific due to the amplification of two different sequences in two separate assays.

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2004-08-01
2019-11-18
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References

  1. Aoyama, T., Tamura, C., Takeuchi, Y., Kamimura, T. & Imaizumi, A. ( 1997;). Rapid, sensitive and specific diagnosis of Bordetella pertussis using the polymerase chain reaction. Acta Paediatr Jpn 39, 44–47.[CrossRef]
    [Google Scholar]
  2. Arico, B. & Rappuoli, R. ( 1987;). Bordetella parapertussis and Bordetella bronchiseptica contain transcriptionally silent pertussis toxin genes. J Bacteriol 169, 2847–2853.
    [Google Scholar]
  3. Backman, A., Johansson, B. & Olcen, P. ( 1994;). Nested PCR optimized for detection of Bordetella pertussis in clinical nasopharyngeal samples. J Clin Microbiol 32, 2544–2548.
    [Google Scholar]
  4. Birkebæk, N. H., Heron, I. & Skjodt, K. ( 1994;). Bordetella pertussis diagnosed by polymerase chain reaction. APMIS 102, 291–294.[CrossRef]
    [Google Scholar]
  5. Christie, C. D., Marx, M. L., Marchant, C. D. & Reising, S. F. ( 1994;). The 1993 epidemic of pertussis in Cincinnati.Resurgence of disease in a highly immunized population of children. N Engl J Med 331, 16–21.[CrossRef]
    [Google Scholar]
  6. Cimolai, N., Trombley, C. & O'Neill, D. ( 1996;). Diagnosis of whooping cough: a new era with rapid molecular diagnostics. Pediatr Emerg Care 12, 91–93.[CrossRef]
    [Google Scholar]
  7. Douglas, E., Coote, J. G., Parton, R. & McPheat, W. ( 1993;). Identification of Bordetella pertussis in nasopharyngeal swabs by PCR amplification of a region of the adenylate cyclase gene. J Med Microbiol 38, 140–144.[CrossRef]
    [Google Scholar]
  8. Faber, M., Lind, I., Rancke-Madsen, A. & Christensen, M. ( 1997;). Whooping cough 1996. Epi-News 41.
  9. Farizo, K. M., Cochi, S. L., Zell, E. R., Brink, E. W., Wassilak, S. G. & Patriarca, P. A. ( 1992;). Epidemiological features of pertussis in the United States, 1980–1989. Clin Infect Dis 14, 708–719.[CrossRef]
    [Google Scholar]
  10. Farrell, D. J., McKeon, M., Daggard, G., Loeffelholz, M. J., Thompson, C. J. & Mukkur, T. K. ( 2000;). Rapid-cycle PCR method to detect Bordetella pertussis that fulfils all consensus recommendations for use of PCR in diagnosis of pertussis. J Clin Microbiol 38, 4499–4502.
    [Google Scholar]
  11. Fine, P. E. & Clarkson, J. A. ( 1986;). Seasonal influences on pertussis. Int J Epidemiol 15, 237–247.[CrossRef]
    [Google Scholar]
  12. Glare, E. M., Paton, J. C., Premier, R. R., Lawrence, A. J. & Nisbet, I. T. ( 1990;). Analysis of a repetitive DNA sequence from Bordetella pertussis and its application to the diagnosis of pertussis using the polymerase chain reaction. J Clin Microbiol 28, 1982–1987.
    [Google Scholar]
  13. Grimprel, E., Begue, P., Anjak, I., Betsou, F. & Guiso, N. ( 1993;). Comparison of polymerase chain reaction, culture, and western immunoblot serology for diagnosis of Bordetella pertussis infection. J Clin Microbiol 31, 2745–2750.
    [Google Scholar]
  14. He, Q., Mertsola, J., Soini, H., Skurnik, M., Ruuskanen, O. & Viljanen, M. K. ( 1993;). Comparison of polymerase chain reaction with culture and enzyme immunoassay for diagnosis of pertussis. J Clin Microbiol 31, 642–645.
    [Google Scholar]
  15. Heininger, U., Schmidt-Schlapfer, G., Cherry, J. D. & Stehr, K. ( 2000;). Clinical validation of a polymerase chain reaction assay for the diagnosis of pertussis by comparison with serology, culture, and symptoms during a large pertussis vaccine efficacy trial. Pediatrics 105, E31. E31.[CrossRef]
    [Google Scholar]
  16. Jensen, J. S., Uldum, S. A., Sondergard-Andersen, J., Vuust, J. & Lind, K. ( 1991;). Polymerase chain reaction for detection of Mycoplasma genitalium in clinical samples. J Clin Microbiol 29, 46–50.
    [Google Scholar]
  17. Jensen, J. S., Borre, M. B. & Dohn, B. ( 2003;). Detection of Mycoplasma genitalium by PCR amplification of the 16S rRNA gene. J Clin Microbiol 41, 261–266.[CrossRef]
    [Google Scholar]
  18. Kristensen, K. H. ( 1961;). Sammenligning af substrater til transport og dyrkning af kighostebakterier. Ugeskr Laeger 123, 569–575 (in Danish).
    [Google Scholar]
  19. Li, Z., Jansen, D. L., Finn, T. M., Halperin, S. A., Kasina, A., O'Connor, S. P., Aoyama, T., Manclark, C. R. & Brennan, M. J. ( 1994;). Identification of Bordetella pertussis infection by shared-primer PCR. J Clin Microbiol 32, 783–789.
    [Google Scholar]
  20. Lichtinghagen, R., Diedrich-Glaubitz, R. & von Horsten, B. ( 1994;). Identification of Bordetella pertussis in nasopharyngeal swabs using the polymerase chain reaction: evaluation of detection methods. Eur J Clin Chem Clin Biochem 32, 161–167.
    [Google Scholar]
  21. Loeffelholz, M. J., Thompson, C. J., Long, K. S. & Gilchrist, M. J. ( 1999;). Comparison of PCR, culture, and direct fluorescent-antibody testing for detection of Bordetella pertussis. J Clin Microbiol 37, 2872–2876.
    [Google Scholar]
  22. Mastrantonio, P., Stefanelli, P. & Giuliano, M. ( 1996;). Polymerase chain reaction for the detection of Bordetella pertussis in clinical nasopharyngeal aspirates. J Med Microbiol 44, 261–266.[CrossRef]
    [Google Scholar]
  23. Mazengia, E., Silva, E. A., Peppe, J. A., Timperi, R. & George, H. ( 2000;). Recovery of Bordetella holmesii from patients with pertussis-like symptoms: use of pulsed-field gel electrophoresis to characterize circulating strains. J Clin Microbiol 38, 2330–2333.
    [Google Scholar]
  24. McLafferty, M. A., Harcus, D. R. & Hewlett, E. L. ( 1988;). Nucleotide sequence and characterization of a repetitive DNA element from the genome of Bordetella pertussis with characteristics of an insertion sequence. J Gen Microbiol 134, 2297–2306.
    [Google Scholar]
  25. Muller, A. S., Leeuwenburg, J. & Pratt, D. S. ( 1986;). Pertussis: epidemiology and control. Bull World Health Organ 64, 321–331.
    [Google Scholar]
  26. Qin, X., Turgeon, D. K., Ingersoll, B. P., Monsaas, P. W., Lemoine, C. J., Tsosie. T., Stapp, L. O. & Abe, P. M. ( 2002;). Bordetella pertussis PCR: simultaneous targeting of signature sequences. Diagn Microbiol Infect Dis 43, 269–275.[CrossRef]
    [Google Scholar]
  27. Reischl, U., Lehn, N., Sanden, G. N. & Loeffelholz, M. J. ( 2001;). Real-time PCR assay targeting IS481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii. J Clin Microbiol 39, 1963–1966.[CrossRef]
    [Google Scholar]
  28. Reizenstein, E., Johansson, B., Mardin, L., Abens, J., Mollby, R. & Hallander, H. O. ( 1993;). Diagnostic evaluation of polymerase chain reaction discriminative for Bordetella pertussis, B.parapertussis, and B. bronchiseptica. Diagn Microbiol Infect Dis 17, 185–191.[CrossRef]
    [Google Scholar]
  29. Roeder, B. L. ( 199;). (editor) . Pertussis-charcoal plate. In Handbook of Culture Media, p. 46. Copenhagen: Statens Serum Institut.
  30. Schlapfer, G., Cherry, J. D., Heininger, U., Uberall, M., Schmitt-Grohe, S., Laussucq, S., Just, M. & Stehr, K. ( 1995;). Polymerase chain reaction identification of Bordetella pertussis infections in vaccinees and family members in a pertussis vaccine efficacy trial in Germany. Pediatr Infect Dis J 14, 209–214.[CrossRef]
    [Google Scholar]
  31. Schmidt-Schlapfer, G., Liese, J. G., Porter, F., Stojanov, S., Just, M. & Belohradsky, B. H. ( 1997;). Polymerase chain reaction (PCR) compared with conventional identification in culture for detection of Bordetella pertussis in 7153 children. Clin Microbiol Infect 3, 462–467.[CrossRef]
    [Google Scholar]
  32. Shepard, C. W., Daneshvar, M. I., Kaiser, R. M. & 7 other authors ( 2004;). Bordetella holmesii bacteremia: a newly recognized clinical entity among asplenic patients. Clin Infect Dis 38, 799–804.[CrossRef]
    [Google Scholar]
  33. Templeton, K. E., Scheltinga, S. A., van der Zee, A., Diederen, B. M., Kruijssen, A. M., Goossens, H., Kuijper, E. & Claas, E. C. ( 2003;). Evaluation of real-time PCR for detection of and discrimination between Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii for clinical diagnosis. J Clin Microbiol 41, 4121–4126.[CrossRef]
    [Google Scholar]
  34. Tilley, P. A., Kanchana, M. V., Knight, I., Blondeau, J., Antonishyn, N. & Deneer, H. ( 2000;). Detection of Bordetella pertussis in a clinical laboratory by culture, polymerase chain reaction, and direct fluorescent antibody staining; accuracy, and cost. Diagn Microbiol Infect Dis 37, 17–23.[CrossRef]
    [Google Scholar]
  35. van der Zee, A., Agterberg, C., Peeters, M., Schellekens, J. & Mooi, F. R. ( 1993a;). Polymerase chain reaction assay for pertussis: simultaneous detection and discrimination of Bordetella pertussis and Bordetella parapertussis. J Clin Microbiol 31, 2134–2140.
    [Google Scholar]
  36. van der Zee, A., Agterberg, C., van Agterveld, M., Peeters, M. & Mooi, F. R. ( 1993b;). Characterization of IS1001, an insertion sequence element of Bordetella parapertussis. J Bacteriol 175, 141–147.
    [Google Scholar]
  37. van der Zee, A., Agterberg, C., Peeters, M., Mooi, F. & Schellekens, J. ( 1996;). A clinical validation of Bordetella pertussis and Bordetella parapertussis polymerase chain reaction: comparison with culture and serology using samples from patients with suspected whooping cough from a highly immunized population. J Infect Dis 174, 89–96.[CrossRef]
    [Google Scholar]
  38. van der Zee, A., Mooi, F., Van Embden, J. & Musser, J. ( 1997;). Molecular evolution and host adaptation of Bordetella spp.: phylogenetic analysis using multilocus enzyme electrophoresis and typing with three insertion sequences. J Bacteriol 179, 6609–6617.
    [Google Scholar]
  39. Wirsing von König, C. H., Hoppe, J. E., Tacken, A. & Finger, H. ( 1990;). Detection of Bordetella pertussis in clinical specimens. In Proceedings of the 6th International Symposium on Pertussis, pp. 315–321. Bethesda, MD: US FDA.
  40. Yih, W. K., Silva, E. A., Ida, J., Harrington, N., Lett, S. M. & George, H. ( 1999;). Bordetella holmesii-like organisms isolated from Massachusetts patients with pertussis-like symptoms. Emerg Infect Dis 5, 441–443.[CrossRef]
    [Google Scholar]
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