1887

Abstract

Although infection of pigs with Typhimurium represents a serious problem, most studies on infection have been carried out in other species. The purpose of the current study was to examine the route(s) of entry of Typhimurium in pigs, using a jejunal loop model. The infection process was followed over 240 min using single to triple immunocytochemical detection of and intestinal cell markers. invasion was observed in both cytokeratin-18-positive and -negative cylindrical absorptive cells within 5–10 min. Subepithelial invasion of ordinary villi was consistently less marked than invasion of the subepithelial layer of Peyer's patches. Our results show that several epithelial cell types were invaded by , and that Peyer's patches represent the main portal of entry in early infection. Additionally, infection was associated with alterations in the keratin and F-actin cytoskeleton of intestinal epithelial cells, probably reflecting toxin-mediated actions. Such changes were confined to the proximal region of the jejunum, demonstrating a regional heterogeneity of intestinal epithelial cell responses to infection.

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2004-07-01
2019-11-19
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